Effect of pindolol on endocrine and temperature responses to buspirone in healthy volunteers

Ten healthy subjects received buspirone (30 mg orally) with and without pre-treatment with the 5-HT1A receptor antagonist, pindolol (80 mg over 3 days). Following pindolol treatment the growth hormone and hypothermic responses to buspirone were significantly decreased. There was also a delay in the onset of the prolactin response to buspirone but the total amount of prolactin secretion, calculated as area under the curve, was not significantly reduced. The data suggest that the growth hormone and hypothermic responses to buspirone in humans are mediated by 5-HT1A receptors, but an explanation founded on pharmacokinetic factors cannot presently be excluded. Both this latter possibility and the lack of selectivity of pindolol for 5-HT receptors indicate the need for the further neuroendocrine studies of the mode of action of buspirone, preferably with more selective 5-HT1A receptor antagonists.     

Carbohydrate metabolism in children receiving growth hormone for 5 years

Carbohydrate metabolism was evaluated by fasting and postprandial glucose, insulin, and hemoglobin (Hb)A_1c levels in children with chronic renal insufficiency and various other growth disorders treated with growth hormone. Mean fasting and postprandial glucose remained unchanged throughout the 5-year study period in all four study groups. Median fasting insulin levels rose from lownormal levels into the normal range after 5 years of growth hormone. Average fasting insulin level after 5 years was 10 mU/l. Median postprandial insulin values also rose, yet remained within the normal range at the 5-year mark. Mean Hb A_1c levels remained within low to middle end of the normal range in the patients with growth hormone deficiency, Turner syndrome, and idiopathic short stature. Mean Hb A_1c levels at the 5 years were slightly elevated to 6.3% for the patients with chronic renal insufficiency.     

生长激素在体外对人胃癌细胞的作用

目的　研究重组人生长激素 (rhGH)在体外对人胃癌细胞生长的影响。方法　实验分组 :对照组、rhGH组、奥沙利铂 (L OHP)组和rhGH L OHP组 ,利用体外细胞培养、MTT比色技术及流式细胞仪等方法 ,测定不同浓度的rhGH对人胃癌细胞株BGC82 3生长曲线、细胞抑制率、细胞周期和增殖指数 (PI)的影响。结果　rhGH在体外无明显促进BGC82 3细胞分裂增殖 ,rhGH组与对照组、rhGH L OHP组与L OHP组比较差异均无显著性 (P >0 .0 5 ) ,且生长曲线没有升高 ;rhGH L OHP组与对照组比较或rhGH L OHP组与对应的rhGH组配对比较 ,细胞抑制率增加 ,阻滞于G0 ～G1期的细胞数增加 ,S期细胞明显减少 ,PI明显降低 (P <0 .0 1)。rhGH L OHP组与L OHP组比较 ,细胞抑制率呈现升高趋势 ,而PI呈下降趋势 ,提示rhGH与抗肿瘤药合用可以增强抗肿瘤药对肿瘤细胞的杀伤作用。结论　rhGH在体外无明显促进胃癌细胞的分裂增殖 ,与抗肿瘤药合用可提高抗肿瘤药的疗效。     

牛黄栀子配伍对大鼠局灶性脑缺血再灌注不同时段神经生长因子（NGF）含量的影响

目的:通过检测牛黄、栀子配伍对大鼠局灶性脑缺血再灌注不同时段神经生长因子(NGF)含量的影响,探讨治疗缺血性中风在该作用环节不同时段的作用特点,研究其配伍阻抑脑缺血级联反应的时序特征。方法:用线栓法建立大鼠大脑中动脉缺血再灌注模型,观察牛黄、栀子及二者配伍对缺血再灌注24小时和72小时两个时段大鼠脑组织NGF含量的影,响。结果:缺血再灌注损伤可明显抑制脑组织NGF的表达,从而削弱了机体的自身保护机制;再灌注24小时,牛黄、栀子均可促进NGF的表达,而二者配伍后作用明显降低:再灌注72小时,牛黄的作用不显著,栀子可明显增强NGF的表达,二者配伍与栀子的作用相当,未显示出明显的配伍效应。结论:中药配伍的作用不是简单的效应累加,而是依据作用环节及作用时间的不同表现为不同的时空特征。     

二十二碳六烯酸对神经细胞生长发育的影响

目的：观察二十二碳六烯酸（DHA）对神经细胞生长发育的影响。方法：将无血清培养的新生大鼠大脑神经细胞分为实验组和对照组，实验组加入0.33mol/L乳化DHA 40μl。对两组细胞进行形态学观察及蛋白质含量测定。结果：实验组大脑神经细胞的突起生长速度、胞体面积和直径、神经细胞存活率及蛋白质含量均显著高于对照组。结论：DHA对神经细胞的生长有促进作用，这一作用可能与促进神经细胞蛋白质合成有关。     

Naturally occuring antibodies against nerve growth factor in human and rabbit sera: comparison between control and herpes simplex virus-infected patients

Antibodies against nerve growth factor (NGF) in sera were detected by enzyme-linked immunosorbent assays (ELISA), by their isolation after passage of sera through NGF immunoadsorbent columns and by their specificity to bind and immunoprecipitate mouse NGF as well as to stain by immunohistochemical methods cellular sites of NGF synthesis. Increased levels of anti-NGF antibodies were found in sera of herpes simplex virus (HSV)-infected patients but not in HSV-inoculated rabbits. As HSV latency is known to be promoted by NGF in vitro, these results may suggest that anti-NGF antibodies modulate the cytokine function of NGF and thus might play a role in HSV infection. The biological function of circulating antibodies against NGF, in general, is now open to future investigation.     

Capillary hemangioblastoma: histogenesis of stromal cells

Summary The histogenesis of stromal cells in capillary hemangioblastoma has been the subject of debate. The light and electron microscopic studies of hemangioblastomas presented here showed pericytic and leiomyoblastic features in stromal cells. Cells cultured by the monolayer method showed similar features to those of the original tumors. Immunohistochemical studies for glial fibrillary acidic protein and factor VIII/von Willebrand factor indicated that stromal cells were antigenically distinct from astrocytes and endothelial cells. These findings suggest that stromal cells are closely related to pericytes and smooth muscle cells, and support Rhodin's speculation that pericytes serve as a precursor to smooth muscle cells.     

广州市大学生尝试自杀现况及其影响因素分析

目的了解广州市大学生尝试自杀现状及其影响因素，为高校健康教育提供理论依据。方法采用整群随机抽样方法，用自编调查表对大学生进行问卷调查，在此基础上结合专题小组讨论的定性研究方法进行分析。结果大学生近1a内尝试自杀报告率为6．0％，不同性别、学校、专业的学生尝试自杀报告率差异无统计学意义（P〉0．05）；城镇学生尝试自杀报告率为6．6％，高于农村学生的4．2％（x^2=4．71，P=0．032）。对自杀行为的态度、朋友和家人有过自杀行为、消极应对、负性生活事件等是尝试自杀的危险因素，获得社会支持是尝试自杀的保护因素。定性访谈发现，学校几乎没有开展过自杀行为的健康教育活动，绝大部分学生对自杀行为持中立态度。结论加强健康教育、消除危险因素、提高保护因素可以最大限度地预防自杀.     

颅盖畸形形成机制的研究进展

目的为深入了解颅盖畸形目前在国内外研究中的进展情况,探讨颅缝早闭过程中相关生长因子的作用,为今后的研究工作奠定基础。方法应用计算机检索PubMed数据库,检索英文相关文章,同时检索中国期刊全文数据库的相关中文文章,并查阅相关书籍。对资料进行初审,并查看文献后的引文。纳入与颅盖畸形形成机制有关的内容,排除不相关及重复文章。结果经对收集文章的整理,共纳入23篇文献。对纳入的颅盖畸形形成机制的文献,结合目前国内外研究状况进行综述。结论进一步研究颅缝过早闭合及相关生长因子的作用,将有助于阐明颅盖畸形形成机制,并更好地指导临床治疗。