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101.
Mohammed AL-JUMAILY Alexei KOZLENKOV Ilana MECHALY Agnes FICHARD Valerie MATHA Frederique SCAMPS Jean VALMIER Patrick CARROLL 《中国神经科学杂志》2007,(5)
目的在背根神经节(dorsal root ganglion,DRG)中等大小感觉神经元中可以观察到钙激活氯离子流(I_(Cl(Ca)))。在坐骨神经损伤模型中,在大多数大中神经元上诱导出类似的氯离子流。本文旨在探讨引起这个离子流的分子基础。方法使用常规的定量RT-PCR方法检测在DRG中三个基因家族的表达,这三个基因家族都具有诱导I_(Cl(Ca))的特点。结果在成年小鼠的DRG中,分别显示了在正常状态和坐骨神经损伤3天后CLCA,Bestrophin和Tweety基因家族成员的转录产物。结论mBestl和Tweety2可能在损伤诱导的DRG神经元I_(Cl(Ca))中发挥作用。 相似文献
102.
目的:观察加味半夏白术天麻汤治疗风痰阻络型脑梗塞的临床疗效及对血液流变学、血清超敏C反应蛋白(he—CRP)的影响。方法:对照组给予阿斯匹林片0.1g,日1次;胞二磷胆碱0.5g加入生理盐水100ml中静脉点滴,日1次;脑水肿明显者应用甘露醇脱水治疗。治疗组在对照组基础上加用半夏白术天麻汤加味。连续治疗一个月后进行临床疗效及神经功能的评定,治疗前后检查血液流变学及he—CRP。结果:治疗组总有效率95%,对照组81.67%,治疗组优于对照组,有显著差异(P〈0.05);在改善神经功能评分、血液流变学及he—CRP方面,治疗组均优于对照组,有显著性差异(P〈0.05,或P〈0.01)。结论:加味半夏白术天麻汤治疗风痰阻络型脑梗塞临床疗效较好,并能减少神经功能缺损程度评分,改善血液流变学及降低he—CRP。 相似文献
103.
利用于氨三乙酸存在下,锰(Ⅱ)对高碘酸钾氧化二甲苯蓝FF的催化效应,建立了一个新的测定微量锰的动力学分光光度法。该法检出限为6×10 ̄(-2)ng·ml ̄(-1),线性范围为0.2~8.0ng·2.5ml ̄(-1),常见离子基本不干扰测定。用于直接测定健齿茶和茉莉花茶中的锰含量,结果发现,锰含量分别为2214μg·g ̄(-1)及648μg·g ̄(-1)。此法简便易行,灵敏度高。 相似文献
104.
Summary Based on the gate-related receptor hypothesis, an analysis of kinetics of AN-132, a new antiarrhythmic agent, blockade of
cardiac sodium channels and the gate-related receptor which is bound by the drug was performed by computer simulation. Model-predicted
apparent rates of onset of AN-132 (30 μmol/L) blocking were 0.051, 0.038, and 0.034 AF−1 at stimulation frequencies of 1.0, 2.0 and 3.0 Hz, respectively. The time constant of recovery from block by AN-132 at resting
potential -90 mV was 39.5 s. These findings are in agreement with those experimental data documented. The analysis of gate-related
receptor shows that AN-132 binds the inactivation gate-related receptor, and the binding and unbinding are modulated by the
inactivation process. 相似文献
105.
106.
J.Martinez Calatayud C.Gomez Benito D.Gaspar Gimenez 《Journal of pharmaceutical and biomedical analysis》1990,8(8-12):667-670
A flow injection-fluorimetric determination of thiamine is reported. The procedure is based on the oxidation of the analyte with potassium hexacyanoferrate(III) immobilized on an anionic exchange resin; the fluorescence is monitored in aqueous basic solution. Concentrations of the vitamin of 0.1–4 ppm have been determined; the relative standard deviation was 1.8%. The injection rate was 28 samples/h. The influence of other substances and the determination of the drug in a pharmaceutical formulation are also reported. 相似文献
107.
肖永俭 《山东中医药大学学报》1990,(1)
通过大量循经疼痛病例观察,认为循经疼痛与经循线上的损伤、大脑皮层机能、植物神经机能状态、植物神经末梢结构的特殊联系有关。提出探求循经疼痛的病因对研究牵涉痛与经络的关系、防止手术损伤经络、治疗某些顽固性疼痛有重要意义。 相似文献
108.
目的研究内毒素、失血性休克大鼠肝线粒体质子跨膜转运和H+-ATP酶的变化。方法大肠杆菌内毒素休克模型和失血性休克模型。采用荧光探针ACMA测定质子跨膜转运。结果(1)内毒素休克5小时亚线粒体在以ATP、NADH和succinate为底物时引起的ACMA最大荧光淬灭值显著减少(P<0.05);最大荧光淬灭时间和半数荧光淬灭时间非常显著延长(P<0.01)。(2)内毒素休克早期,线粒体H+-ATP酶活性显著升高(P<0.05),晚期非常显著下降。(3)内毒素休克大鼠肝线粒体膜结合PLA2、血浆和线粒体MDA升高(P<0.05)。(4)失血性休克时H+-ATP酶和质子转运无显著改变。结论内毒素休克时线粒体跨膜质子转运和H+-ATP酶活性显著下降,失血性休克时变化不大 相似文献
109.
The differential effects of the pyrethroid tetramethrin on tetrodotoxin-sensitive (TTX-S) and tetrodotoxin-resistant (TTX-R) single sodium channel currents in rat dorsal root ganglion (DRG) neurons were investigated using the outside-out configuration of patch-clamp technique. Channel conductances were 10.7 and 6.3 pS for TTX-S and TTX-R sodium channels, respectively, at a room temperature of 24–26°C. The single-channel current of TTX-S sodium channels at the test potential of −30 mV was −1.27 ± 0.25 pA, and was not changed after exposure to 10 μM tetramethrin (−1.28 ± 0.23 pA). The open time histogram of TTX-S single-channel currents could be fitted by a single exponential function with a time constant of 1.27 ms. After exposure to 10 μM tetramethrin, the open time histogram could be fitted by the sum of two exponential functions with time constants of 1.36 ms (τfast) and 5.73 ms (τlow). The percentage of contribution of each component to the population was 62% for the fast component representing the normal channels and 38% for the slow component representing the tetramethrin modified channels. The amplitudc of TTX-R single-channel currents was slightly changed from −0.72 ± 0.14 to −0.83 ± 0.07 pA by 10 μM tetramethrin. The open time histogram of TTX-R single-channel currents could be fitted by a single exponential function with a time constant of 1.92 ms. In the presence of 10 μM tetramethrin, the open time histogram could be fitted by the sum of two exponential functions with time constants of 2.07 ms (τfast) and 9.75 ms (τslow). The percentage of contribution of each component was 15% for the fast, unmodified component and 85% for the slow, modified component. Differential effects of tetramethrin on the open time distribution of single sodium channel currents explains the differential sensitivity of TTX-S and TTX-R sodium channels. 相似文献
110.
Michael Madeja Ulrich Mußhoff Christoph Lorra Olaf Pongs Erwin-Josef Speckmann 《Brain research》1996,722(1-2)
The action of the epileptogenic agent pentylenetetrazol (PTZ) on a cloned potassium channel of the rat brain was studied. The Kv1.1 channel was expressed in oocytes ofXenopus laevis and potassium currents were investigated in outside-out and inside-out membrane patches. The results show that PTZ increased the multi-channel potassium currents at strongly negative potentials and decreased them at potentials positive to −35 mV both in outside-out and inside-out membrane patches. The extent and manner of PTZ action, the concentration dependence as well as the onset and time course of the PTZ effect were the same both in outside-out and inside-out membrane patches. The single-channel potassium currents showed an increase in open probability and frequency of opening and a decrease in close time at −50 mV and vice versa at 0 mV with application of PTZ. The amplitude of single-channel current, the open time and the latency to the first channel opening remained almost unchanged under PTZ. The results indicate that PTZ acts via the cell membrane and influences the membrane-associated part of the potassium channel. Thereby, PTZ accelerates the transition from the inactivated to the open state of the channel at strongly negative potentials and reduces it at slightly negative and positive potentials. This mechanism may be the basis for a gate function which is in favour of the development of epileptic discharges. 相似文献