Removal of reactive yellow 145 by adsorption onto treated watermelon seeds: Kinetic and isotherm studies

The aim of this study is to valorize the watermelon seeds treated with hexane for the removal of reactive yellow 145 from aqueous solutions. The effects of physico-chemical parameters such as pH, adsorbent dose, temperature and dye concentration on the removal of dyes were evaluated. The kinetic study of the adsorption of reactive yellow 145 on watermelon seeds adsorbent perfectly followed the pseudo-second-order kinetic model. The Langmuir, Freundlich, Temkin, Elovich and Dubinin-Radushkevich models were studied. It was proved that the experimental data fitted well to the Freundlich model, which signifies that the phenomenon of adsorption is done in multilayers on heterogeneous surfaces. The finding of this paper asserts that this material is a good and low-cost adsorbent that can be used for the removal of dyes from aqueous solutions.     

贫铀武器对人体健康和环境危害的研究进展

介绍了贫铀的化学特性、衰变规律和迁移方式。依据国内外有关贫铀的实验数据、流行病学研究、动物毒理学试验和人体临床观察等几个方面的研究结果 ,综述了贫铀武器对人体和环境的影响 ,结合当前研究中的不确定因素 ,提出了今后进行有关研究的建议。     

细胞角质素20和存活素在膀胱癌诊断中的价值

【目的】评价细胞角质素20(CK20)和存活素(Survivin)在膀胱癌诊断中的价值。【方法】采用前瞻性临床研究的方法,应用RT-PCR技术,对膀胱癌病人、泌尿系良性疾病病人和健康志愿者进行尿脱落细胞CK20mRNA、Survivin mRNA检测和尿细胞学(UC)检查,比较其在膀胱癌诊断中的价值。【结果】尿脱落细胞CK20mRNA、Survivin mRNA检测和UC检查的敏感性分别为86.1%、83.3%、31.9%,特异性分别为63.4%、90.2%、100%。【结论】尿脱落细胞CK20mRNA、Survivin mRNA检测的敏感性均明显高于尿细胞学检查,可以替代尿细胞学检查,作为膀胱镜检查的辅助手段。Survivin mRNA检测具有较高的特异性,适用于膀胱癌的临床诊断。     

杜惠兰运用经方治疗妇科疾病经验

经方被誉为"众法之宗,群方之祖",其组方严谨,选药精简,效如桴鼓,备受后世医家推崇。杜惠兰教授从医三十余载,学验俱丰,临证善用经方治疗妇科诸疾,疗效显著。文章介绍杜惠兰教授秉承辨证论治基本原则及辨病与辨证相结合,灵活化裁运用经方治疗痛经、卵巢囊肿、盆腔炎性疾病以及妇人脏躁验案。     

Knocking-out of H1F1α gene by CRISPR/cas9 inhibits proliferation and invasiveness of prostate cancer DU145 cellsCSCD

Objective To explore the role of H1F1α gene in prostate cancer cell line DU145 by knocking it out with a novel gene-editing tool CRISPR/cas9 system. Methods A CRISPR/cas9 system with two sgRNAs targeting exon 1 of the H1F1α gene was constructed for the knock out experiment. CCK8 assay and transwell experiment were carried out to assess the effect of the knock out on the proliferation, migration and invasiveness of DU145 cells. Results The efficiency of gene-targeting was measured through a T7E1 assaying and sequence analysis, which confirmed that the partial knock out was successful and has led to a significant decrease in the expression of H1F1α and inhibition of cell proliferation, migration and invasiveness. Conclusion A CRISPR/cas9 system for the knock out of H1F1α has been successfully constructed, which could inhibit the proliferation and migration of DU145 cells. The system can facilitate further studies of the H1F1α gene and its roles in tumorigenesis. © 2018 West China University of Medical Sciences. All rights reserved.     

电针至阳穴对急性心肌缺血家兔血浆cAMP、cGMP含量的影响

目的 观察电针至阳穴对急性心肌缺血家兔血浆环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）含量的影响。方法 40只家兔随机分为4组：正常对照组（A组）、单纯造模组（B组）、造模＋电针内关穴组（C组）、造模＋电针至阳穴组（D组），采用垂体后叶素（2U／kg）造成家兔心肌急性缺血，在全过程中多次测定Ⅱ导联心电图，并用放射免疫法测定电针后家兔血浆cAMP、cGMP含量。结果 C组和D组家兔血浆中cAMP含量明显低于B组（P〈0．01），与A组比较无显著性差异（P〉0．05），C组与D组间亦无显著性差异（P〉0．05）；各组间cGMP含量无显著性差异（P〉0．05）。结论 电针至阳穴可提高家兔心肌对缺血缺氧的耐受力．改善心肌的缺血缺氧状态，与电针内关穴有同等效果。     

西药联合中药消溃平汤剂治疗消化性溃疡的疗效观察与护理

目的 探讨中药消溃平汤剂治疗胃及十二指肠溃疡疗效及护理措施.方法 随机将85例消化性溃疡患者分为治疗组53例和对照组32例.两组均服用奥美拉唑、阿莫西林、克拉霉素,治疗组另加服中药消溃平汤剂.疗程4周.疗程结束后比较两组治疗有效率、复发率及幽门螺杆菌(Hp)根除率.结果 两组治愈有效率、复发率、Hp根除效果均有统计学意义(P＜0.05).结论 中药消溃平汤剂联合西药治疗胃及十二指肠溃疡比单用西药在加速溃疡面愈合,增强对Hp的根除作用,减少复发有显著的协同作用.护理上指导患者正确服食汤药有助于药效发挥.     

BDNF-AS/miR-145-5p轴对高糖诱导的肾小管上皮细胞损伤的影响CSCD

目的探讨脑源性神经营养因子反义RNA(brain-derived neurotrophic factor-antisense, BDNF-AS)对高糖诱导的肾小管上皮细胞损伤的影响和可能机制。方法体外培养肾小管上皮细胞HK-2, 分别转染BDNF-AS小干扰RNA、miR-145-5p模拟物或共转染BDNF-AS小干扰RNA和miR-145-5p抑制剂, 之后采用30 mmol/L葡萄糖干预转染后的细胞24 h, 用RT-qPCR法检测细胞中BDNF-AS和miR-145-5p的表达, 用CCK-8法检测细胞增殖, 流式细胞术检测细胞凋亡, Western印迹法检测细胞中Bcl-2和Bax蛋白的表达, 酶联免疫吸附法检测细胞培养上清中IL-1β和IL-6的水平。用双荧光素酶报告基因实验验证BDNF-AS和miR-145-5p的调控关系。结果高糖处理促进了HK-2细胞中BDNF-AS的表达(P<0.05), 而抑制了miR-145-5p的表达(P<0.05)。干扰BDNF-AS或过表达miR-145-5p降低了高糖诱导的HK-2细胞抑制率、凋亡率及Bax蛋白、IL-1β和IL-6的表达(P<0.05), 促进了Bcl-2蛋白的表达(P<0.05)。干扰miR-145-5p逆转了干扰BDNF-AS对高糖诱导的HK-2细胞增殖、凋亡及IL-1β和IL-6表达的影响。BDNF-AS可靶向结合并负调控miR-145-5p。结论干扰BDNF-AS可能通过靶向负调控miR-145-5p促进高糖诱导的肾小管上皮细胞增殖, 并抑制细胞凋亡及炎症因子表达。     

In vitro and in vivo evaluation of DU6859a,a new fluoroquinolone,among injectable antibacterials tested against clinical isolates

DU6859a was evaluated for its in vitro and in vivo antibacterial activities in comparison with those of imipenem, meropenem, cefpirome, vancomycin, gentamicin, ciprofloxacin and levofloxacin. DU6859a had activity comparable to that of imipenem against methicillin-susceptible staphylococci and penicillin-susceptible and-resistantStreptococcus pneumoniae, with MICs at which 90% of strains tested are inhibited (MIC₉₀)≤0.063μg/mL. Against methicillin-resistant staphylococci and enterococci, DU6859a was as active as vancomycin and more active than other drugs tested, with MIC₉₀s ranging from 1 to 4 μg/mL. DU6859a was as active as ciprofloxacin and more active than other drugs tested against imipenem-resistantPseudomonas aeruginosa. Differences in activity between DU6859a and reference drugs against ciprofloxacin-resistant and gentamicin-resistantP. aeruginosa were particularly striking. The in vivo efficacy of subcutaneous injections of DU6859a against experimental septicemia, respiratory and pyelonephritic infections caused by gram-positive and-negative bacteria, including methicillin-resistantStaphylococcus aureus and imipenem-resistantP. aeruginosa, reflected its potent in vitro activity.     

miR-145对血管平滑肌细胞生物活性及SM22α mRNA表达的作用*

目的：探究miR-145 对血管平滑肌细胞（VSMC）的生物活性及对SM22α mRNA 表达的作用。方法： VSMC分为3 组,增殖VSMC组（ 增殖组）、增殖VSMC + 转染miR-145-NC 组（ 空载体组）、增殖VSMC+转染 miR-145 组（ 增殖转染组）。采用RT-PCR 检测VSMC中SM22α mRNA、miR-145 的表达;免疫印迹检测cyclin E、p27 蛋白水平;Transwell 小室检测VSMC侵袭能力;MTT检测VSMC活力;流式细胞术检测VSMC凋亡率。 结果：增殖组VSMC中SM22α mRNA、miR-145 水平与空载体组相比数值较为接近;与增殖组及空载体组相比, 增殖转染组VSMC中miR-145、SM22α mRNA 表达升高。增殖组VSMC中cyclin E、p27 蛋白水平与空载体组相 比无差异;增殖转染组cyclin E 蛋白水平低于空载体组、增殖组,p27 蛋白水平高于空载体组、增殖组。增殖组 VSMC迁移数量与空载体组相比无差异;增殖转染组VSMC迁移数量明显低于空载体组、增殖组。增殖组与空载 体组VSMC在不同时间点的OD 值均较为接近;增殖转染组OD 值在不同时间点均低于空载体组、增殖组。增殖 组VSMC凋亡率与空载体组数值接近,组间比较差距较小;增殖转染组VSMC凋亡率较空载体组和增殖组升高。 结论：过表达miR-145 通过促进SM22α 水平增加,抑制血管平滑肌细胞的增殖,并促进其凋亡。