Effect of dipyridamole with or without aspirin on urine protein excretion in patients with membranous glomerulonephritis

Summary The antiproteinuric effect of the antiplatelet agent dipyridamole has been assessed after inhibiton of thromboxane B₂ (TxB₂) synthesis in 8 patients with confirmed membranous glomerulonephritis. There were three study periods, each of 30 days, and 45 days apart, namely a washout period, treatment with dipyridamole 300 mg/d, and dipyridamole 225 mg/d plus aspirin 150 mg/d. On Days 1 and 30 of each study period serum and urine creatinine, 24-h excretion of protein, creatinine clearance, platelet aggregometry on whole blood and serum TxB₂ were measured. Treatment with dipyridamole alone or with aspirin produced significant inhibition of platelet aggregation and a fall in 24-h protein excretion; the latter amounted to 54% with dipyridamole alone and 56 % with dipyridamole plus aspirin (NS). Dipyridamole plus aspirin caused an 82 % reduction in serum TxB₂.     

补中益气汤治疗慢性乙型肝炎的临床与实验研究

通过对补中益气汤及西药治疗慢性乙型肝炎临床疗效的对比研究,发现补中益气汤能较快改善慢性乙型肝炎的临床症状及体征,改善肝功能,促使乙型肝炎病毒血清学标志好转(HBsAg、HBeAg转阴或滴度下降,抗-HBe或抗-HBs转为阳性),与西药治疗对照组比较有显著性差异(P<0.05)。动物实验观察了补中益气汤对小鼠肝组织DNA、RNA、蛋白质合成的影响,结果表明,补中益气汤对三者的合成均有明显的促进作用。其抗肝炎的作用机制可能与该方增强肝脏蛋白质的合成,促进肝组织的修复,改善机体整体的抗病机能有关。     

Cerivastatin inhibits fetal calf serunvinduced DNA synthesis in cultured rat mesangial cells

SUMMARY: Inhibition of mevalonate synthesis by several statins has been shown to suppress DNA synthesis in glomerular mesangial cells. In the present study, we investigated the effect of a new statin, cerivastatin, on fetal calf serum (FCS)-induced DNA synthesis of cultured rat mesangial cells. Cultured rat mesangial cells were stimulated by 10% FCS in the presence or absence of cerivastatin and mevalonate. 5-bromo-2-deoxyuridine (BrdU) incorporation was used to assess DNA synthesis. the present study showed that 10% FCS caused marked stimulation of DNA synthesis in the mesangial cells. Cerivastatin inhibited FCS-stimulated BrdU incorporation in a dose-dependent manner. IC₅₀ was approximately 1 umol/L. Exogenous mevalonate, farnesyl pyrophosphate and geranylgeranyl pyrophosphate significantly prevented the inhibitory effect of cerivastatin on DNA replication. It appears that cerivastatin, by inhibiting the synthesis of mevalonate, may suppress DNA synthesis in the mesangial cells.     

The Chemical Protecting Group Concept Applied in Crosslinking of Natural Tissues with Glutaraldehyde Acetals

This work describes the results of the controlled crosslinking of collagen matrices by glutaraldehyde based on a double protection strategy, glutaraldehyde acetals and lysine protonation due to the acidic conditions of acetal formation. Materials crosslinked by this approach were characterized by thermal stability comparable to those obtained by conventional procedures with mechanical properties expected for bioprosthesis manufacture and with a higher stability toward collagenase hydrolysis. The integrity of the microfibrillar structure was confirmed by optical and scanning electronic microscopy. The results indicate that the glutaraldehyde acetals procedure may be of potential use for the crosslinking of bovine pericardium used in the manufacture of bioprosthetic devices. Advantages may be related to the production of materials with homogeneous crosslinking distributions, associated with better definition in the nature of the chemical link that they introduce, due to a better distribution of glutaraldehyde within the tissue matrix before the crosslinking reaction is allowed to occur. As a result, materials with improved biological and mechanical properties are expected.     

抗NI-35重组单链抗体基因cDNA的合成与克隆

目的抗NI-35抗体(anti-NI-35antibody)作为神经再生抑制因子(NI-35)的拮抗剂对神经再生促进作用的研究是近年的热点,研究表明,抗NI-35抗体能促进体内外受损伤神经元的存活和突起生长。我们重新设计并人工合成抗NI-35重组单链抗体(anti-NI-35-scfv)的cDNA克隆构建其表达载体,在原核系统中实现初步表达。方法参照genebank中发表的抗NI-35抗体的轻链重链序列,重新设计适于在大肠杆菌中表达的目的基因片段,将该基因双链分成35个小片段合成,经退火、复性连接成目的片段后,克隆到经过BamHI和HindIII双酶切的克隆载体pUC18中,并转化大肠杆菌DH5a,抽提重组子pUC18/744进行克隆PCR、酶切鉴定及测序分析。结果测序结果证明获得的基因序列与实验设计仅差一个碱基。结论正确合成了抗NI-35重组单链抗体(anti-NI-35-single-chainantibody)为抗NI-35抗体应用于治疗弥漫性轴索损伤提供了实验基础。     

超声引导胶原酶治疗外侧型腰椎间盘突出症

目的探讨超声引导胶原酶治疗外侧型腰椎间盘突出症的疗效及安全性.方法在相应椎间盘突出的棘突间,旁开1.5～2.5cm处经皮穿刺,采用超声引导定位,穿刺成功后,先进行加压试验,再进行麻醉试验,无腰麻征象后,将胶原酶注入到突出的椎间盘内或周围.结果 231例病人出院时的优良率为92.21%,有效率为99.57%;随访1～5年的优良率为92.65%,有效率为99.58%.近期与远期疗效比较无显著性差异(χ2=0.129,P＞0.05).结论利用超声引导监测、定位,注射胶原酶治疗外侧型腰椎间盘突出症,安全性强、有效率高.     

Preparation of tritium‐labeled Silybin—a protectant for common liver diseases

Silymarin, the seed extract of milk thistle plant, Silybum marianum, has been used traditionally for the treatment of liver diseases and bile duct infection. Silybin 1 is the main bioactive components of silymarin, consisting a pair of diastereomers: Silybin A and Silybin B. In this article, we report the preparation of tritium‐labeled Silybin, which was accomplished by protection of Silybin as tritylated compound 2 and followed by oxidation of the primary alcohol to its corresponding aldehyde 3 . Subsequent reduction with NaB[³H]₄ and deprotection of the trityl group provided the tritium‐labeled Silybin 4 . Copyright © 2006 John Wiley & Sons, Ltd.     

Twisted plywood architecture of collagen fibrils in human compact bone osteons

Summary Ultrathin sections of decalcified human compact bone, observed by transmission electron microscopy, reveal that collagen fibrils can be distributed in the form of a superimposed series of nested arcs. This characteristic pattern has never been interpreted in previous works on compact bone structure. We demonstrate, by goniometric observations at the ultrastructural level, that such series of nested arcs are a consequence of the “twisted plywood” architecture of collagen fibrils in the compact bone matrix. In the same specimens, an “orthogonal plywood” disposition of collagen fibrils is also observed; a transition exists between these two types of orders. We show that the “twisted plywood structure” accounts well for certain optical properties of osteons, observed in polarizing microscopy, described as “intermediate osteons.” The particular geometry of collagen fibrils, leading to nested arcs in oblique sections, is analogous to the distribution of molecules in certain liquid crystals (called cholesteric liquid crystals). The principle of a liquid crystalline self-assembly of the collagen matrix in bone is therefore discussed.     

煤焦沥青烟雾提取物的细胞毒性和遗传毒性研究

采用人外周血淋巴细胞非程序DNA合成(UDS)试验和人胚肺成纤维细胞转化试验,测试了煤焦沥青烟雾提取物(ECTPF)对人体细胞的细胞毒性和遗传毒性。UDS试验结果表明,ECTPF可使淋巴细胞UDS值明显增加,并有剂量一反应关系。引起半数淋巴细胞死亡的浓度(LC50)为33.8μg/ml。细胞转化试验表明,ECTPF能诱发人胚肺成纤维细胞明显的形态学转化,且转化细胞具有部分恶性转化细胞的特性。引起半数人胚肺成纤维细胞生长抑制的浓度为41.3μg/ml。实验结果提示,ECTPF是一种具有细胞毒性和遗传毒性的物质。     

伊普黄酮的合成

以间苯二酚和苯乙腈所制得的２，４－二羟基苯基苄基酮，与２－溴丙烷反应生成２－羟基－４－异丙氧基苯基苄基酮，后者在吗啉－醋酸存在下与原甲酸三乙酯反应，生成伊普黄酮，总收率５２％。