五仁醇固体分散体的制备及体外溶出度研究

目的将五仁醇制成固体分散体,提高五仁醇中有效成分的体外溶出度。方法采用直接将载体材料溶于五仁醇提取液,然后除去溶剂的方法制备了多种五仁醇固体分散体,以五味子甲素为指标成分,研究并比较了它们的表观溶解度和体外溶出度。结果配比为1∶3的五仁醇-聚乙烯吡咯烷酮K 30(PVP K 30)固体分散体中有效成分的表观溶解度比五仁醇胶囊内容物显著提高,达到5.06μg/mL(水);溶出介质(水)中分散(包括溶解)的小于0.22μm的药物高达43.2%。结论五仁醇-PVP K 30固体分散体能明显提高五仁醇中有效成分的表观溶解度和体外溶出度。     

CD30在变应性鼻炎患者鼻黏膜组织中的表达

目的 :探讨CD30在变应性鼻炎患者鼻黏膜组织中的表达 ,分析其在变应性鼻炎发病中的作用。方法 :应用免疫组织化学SP法和苏木精 伊红染色对 31例变应性鼻炎患者的下鼻甲黏膜 (变应性鼻炎组 )和 2 6例肥厚性鼻炎患者的下鼻甲黏膜 (对照组 )标本中CD30的表达进行检测 ,并进行统计学分析。结果 :变应性鼻炎组CD30细胞数显著高于对照组 ,其差异有统计学意义 (P <0 .0 5 )。结论 :在变应性鼻炎的鼻黏膜组织中存在着Th2细胞主导的黏膜反应 ,通过研究CD30的表达水平可了解变应性鼻炎中Th2反应的状态。     

Responsiveness of the generic EQ-5D summary measure compared to the disease-specific EORTC QLQ C-30

Introduction: We investigated whether the sensitivity of the generic health-related quality of life (HRQoL) EQ-5D summary measure (or index) to detect changes over time in a clinical setting is comparable with that of a disease-specific HRQoL questionnaire. Methods: Patients with liver metastases (n = 75) filled out the five domains of the EQ-5D self-classifier, the EQ VAS, and the EORTC QLQ C-30 (a disease-specific (cancer) HRQoL questionnaire). The HRQoL instruments were completed before intervention, and 1/2 month and 3 and 6 months after intervention. Three analyses were performed. First, the EQ-5D index (based on self-classification) was compared to the EQ VAS. Second, the EQ-5D domains were compared to corresponding EORTC QLQ C-30 scales. Third, EQ-5D index and EQ VAS were compared with the EORTC QLQ C-30 global health-status scale. Effect size was chosen as the metric of responsiveness. Results: The EQ-5D index was slightly less responsive than the EQ VAS. Overall, the responsiveness of the EQ-5D index and EQ VAS was equal to the EORTC QLQ C-30 global health-status scale. Conclusion: Despite its generic principle and the apparent crudeness of its framework, the responsiveness of the EQ-5D proved to be comparable to that of a disease-specific HRQoL questionnaire in this specific clinical setting.     

重组抗原金标免疫渗滤法检测弓形虫感染的研究

目的:建立一种快速、简便的弓形虫病诊断方法。方法:将弓形虫P30重组抗原包被于硝酸纤维膜上,以金标免疫渗滤法(DIGFA)检测兔血清中相应抗体,呈现红色斑点者为阳性。结果:检测弓形虫感染兔血清41份,阳性率为97.56%(40/41),检测正常兔血清20份,感染囊虫兔血清26份,感染血吸虫兔血清40份,感染丝虫兔血清21份,感染肺吸虫兔血清33份,仅2份血吸虫感染兔血清出现交叉反应。结论:弓形虫P30重组抗原金标免疫渗滤法检测弓形虫感染兔有较高的敏感性和特异性。     

SD大鼠体外受精与早期胚胎体外培养体系的初步建立

目的改造IVF-30和HTFplus培养液,观察在改造后培养液里卵的受精和早期胚胎的发育状况,寻找适合SD大鼠体外受精和早期胚胎培养的实验体系。方法采用两种方法(超数排卵及体内成熟)采集成熟卵母细胞用于体外受精。选择两种商品化的培养液IVF-30(Vitrolife),HTFplus(Lifeglobal)。分别在以上的培养基中增加10、20、30mmol/L的NaCl,将培养液的渗透压提升至325~355mOsM之间,观察在改造前与改造后的培养液内SD大鼠卵母细胞受精率和囊胚发育率等指标。结果经改造的培养液HTFplus和IVF-30,体外受精率分别从13%、15%提升至70%、67%。改造后的IVF-30和HTFplus的2细胞,大于4细胞及囊胚的发育率分别为85%,74%,22%;96%,75%,32%。HTFplus,IVF-30经过改造后更适合于SD大鼠的体外受精及早期胚胎培养。与超排相比较更多的在自然周期的体内成熟卵母细胞(16%,28%)发育到了囊胚。结论成功建立了SD大鼠的体外受精和体外培养系统。     

聚乙烯吡咯烷酮K30在甲硝唑片制备中的应用

目的研究辅料聚乙烯吡咯烷酮K30(PVPK30)对甲硝唑片质量的影响.方法依照中国药典2000年版二部测定甲硝唑片的溶出度.结果加入辅料PVPK30的甲硝唑片较原处方的溶出度明显加快,两者T50有显著差异(片1T50=11.5 min,片2T50=6.8 min).结论辅料PVPK30能提高甲硝唑片的质量.     

间变性大细胞淋巴瘤1例报告

介绍诊治的1例间变性大细胞淋巴瘤的临床特征、形态学、免疫表型及遗传学特征,并复习相关文献.     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

浙江省局部地区2002～2003年流行的无菌性脑膜脑炎的病原学检测与分析

浙江省局部地区 2 0 0 2～ 2 0 0 3年多次出现无菌性脑膜脑炎的流行。为对疫情进行病原学检测与分析 ,采集了临床标本 ,采用HEp 2、RD、Vero等细胞分离病毒 ,并用肠道病毒通用引物进行特异性逆转录 聚合酶链反应 (RT PCR)扩增。结果显示 :5 1份脑脊液标本中分离出埃可病毒 30型 (ECHO3 0 ) 5株 ,柯萨奇病毒B组 5型 (Coxsack ievirusB5,Cox B5) 1株 ;6 8份粪便标本中分离出ECHO3 0 34株 ,Cox B51株。RT PCR的结果与上述病毒分离结果相一致。此外 ,对采集的 1 5例患者的急性期、恢复期双份血清测定了特异性中和抗体 ,其中 1 3例患者抗体呈≥ 4倍增长。证实引起浙江省局部地区 2 0 0 2～ 2 0 0 3年无菌性脑膜脑炎的主要病原为ECHO3 0 。     

MiR-30a-5p通过泛素水解酶22抑制人宫颈癌细胞上皮-间质转化功能

【目的】研究microRNA-30a-5p（miR-30a-5p）对人宫颈癌Hela细胞上皮-间质转化功能的影响及其相关机制。【方法】宫颈癌Hela细胞株分别转染目的mir的模拟物和阴性对照模拟物,分别以30a-5p组、NC组命名并标记细胞。同时,以未经过处理的Hela细胞作为对照（Control组）。分别用逆转录-聚合酶链反应法检测各组宫颈癌细胞的miR-30a-5p含量。Transwell实验检测3组细胞迁移能力和侵袭能力。Western-blot法检测3组细胞神经-钙粘素（N-cadherin）、α-连环蛋白（α-Catenin）和泛素水解酶22（USP22）表达水平。运用生物信息学方法预测miR-30a-5p的靶基因。采用Western blot法检测USP22过表达对miR-30a-5p抑制EMT的拮抗作用。双荧光素酶实验检测miR-30a-5p与USP22的关系。建立皮下移植瘤模型观察miR-30a-5p的体内作用。【结果】30a-5p组宫颈癌细胞miR-30a-5p的表达水平明显上调,表达水平为Control组的853.82（862.26~843.11）倍（P＜0.01）。30a-5p组侵袭细胞数量8.17（8.32~8.03）明显低于Control组（P＜0.01）。30a-5p组细胞N-cadherin蛋白的细胞内含量明显下降,α-Catenin蛋白的细胞内含量明显上升,USP22蛋白表达量明显降低。合并USP22过表达处理的30a-5p组宫颈癌细胞中N-cadherin蛋白表达量明显升高,α-Catenin蛋白表达量明显降低。双荧光素酶检验结果显示USP22为miR-30a-5p的下游靶基因（P＜0.01）。30a-5p组皮下移植瘤明显小于Control组（P＜0.01）。与Control组肿瘤组织相比,30a-5p组肿瘤组织miR-30a-5p的相对含量升高,USP22蛋白含量降低,N-cadherin蛋白的含量降低,α-Catenin蛋白含量升高。【结论】miR-30a-5p在宫颈癌Hela细胞中,可能通过靶向识别下游靶基因USP22,进而抑制其翻译。最终实现对宫颈癌细胞EMT过程的抑制。