卵巢癌血管生成的多样性

新血管生成在个体发育、创伤愈合等过程中起着至关重要的作用,也是肿瘤生存、转移、复发的组织基础[1].研究表明,少数极恶性肿瘤存在血管生成(angiogenesis)、血管形成(vasculogenesis)和血管生成拟态(vasculogenic mimicry)等方式,多种血管新生方式与肿瘤的转移、复发等恶性生物行为密切相关.卵巢癌的死亡率在女性生殖道癌瘤中居首位,患者5年生存率长期徘徊在30%左右,最新研究证实,卵巢恶性肿瘤血管生成具有多样性,本文将就卵巢癌不同血管生成方式的研究进展及其与卵巢恶性生物学行为的关系进行综述.     

卵巢癌细胞株冻融抗原负载的树突状细胞诱导CTL抗卵巢癌免疫应答的体外研究

目的研究卵巢癌冻融抗原负载的树突状细胞(dendriticcells,DC)诱导细胞毒性T淋巴细胞(CTL)体外杀伤卵巢癌细胞的细胞毒性效应。方法利用免疫磁珠分离法(MACS)分离纯化脐血CD34 细胞并在体外诱导分化为DC,用反复冻融法从卵巢癌细胞系SKOV3中提取的可溶性相关抗原负载DC。流式细胞学检测负载抗原后DC表面各种分化相关抗原的表达,ELISA法检测DC上清中IL12的表达,混合淋巴细胞反应(MLR)测定DC体外刺激T细胞增殖的能力,MTT法检测抗原负载DC激活的抗原特异性CTL对卵巢癌细胞的杀伤作用。结果与未经抗原负载的DC相比,经卵巢癌抗原负载的DC不仅能更高地表达各种DC分化相关抗原CD1α(73.35%±2.94%vs34.1%±2.35%)、CD83(73.9%±8.46%vs54.68%±3.26%)、CD80(91.95%±2.48%vs52.53%±3.18%)、HLADR(70.05%±2.35%vs48.7%±2.07%)以及CD54(88.9%±5.52%vs71.45%±2.29%),同时具有更强的刺激同种异体T淋巴细胞增殖和IL12分泌的能力(P均<0.05)。此外,卵巢癌细胞SKOV3冻融抗原负载DC激活的CTL在体外对SKOV3的杀伤率为77.35%,显著高于未经抗原负载的DC(P=0.0001)。结论经卵巢癌细胞冻融抗原负载DC激活的CTL在体外具有更强的增殖能力和杀伤卵巢癌细胞的作用。     

用集中注意法对抑郁症患者进行押韵汉字的分听研究

目的:探讨抑郁症患者大脑半球机能不对称的特点.方法:用集中注意法及两种押韵词,对58名健康被试、115名抑郁症患者进行耳优势测试.结果:1.健康组真词测听未见耳优势,而假词测听呈右耳(左半球)优势.2.抑郁症患者真词测听未见耳优势,而假词测听呈增强的右耳(左半球)优势.3.真词测听,共病焦虑障碍组无耳优势,非共病组有增强的右耳(左半球)优势;假词测听,两组均表现为右耳(左半球)优势,非共病组强于共病组.4.HAMD、HAMA、SCL-90评分与真、假词偏侧指数无相关性.结论:1.健康中国人对真词的加工与认知无耳优势,表明健康中国人在加工真词时需要左右两侧半球的协同参与;对假词的加工与认知则表现出右耳(左半球)优势,表明更为纯粹的言语材料的加工以左半球为主.2.抑郁症患者对汉字词的加工与认知,表现出较健康人强的右耳(左半球)优势.3.共病和非共病焦虑障碍者对汉字词的加工与认知,表现出较健康人强的右耳(左半球)优势,而非共病者较共病者右耳(左半球)优势强.4.患者的言语偏侧化指数与症状严重程度无相关性.5.假词为较为理想的分听材料.     

Development of patient derived organoids for cancer drug screening applications

Cancer is a disease characterised by abnormal cell growth that can invade or spread to other regions of the body. Organoids are three-dimensional ex vivo tissue cultures made from embryonic stem cells, induced pluripotent stem cells, progenitor cells or tissue that serve as a physiological model for cancer research. These are designed to recapitulate the in vivo properties of tumours. Importantly, effective recapitulation of the structure of tissues and function is believed to predict patient response, allowing for the creation of personalised therapy in a timely manner that may be used in the clinic. This Review discusses the pre-clinical model and different types of human organoids as models for the development of high throughput drug screening and also aims to highlight how organoids are shaping the future of cancer research.     

Ultrastructure of a virilizing ovarian Leydig-cell-tumor

Summary A case of virilizing ovarian hilus cell tumor (Leydig-cell tumor) in a 37 year old female was studied by light and electron microscopy. The ultrastructural features of this rare and almost allways benign tumor are compared with those reported in the literature and with findings in normal and neoplastic interstitial cells of the testis. Tubulovesicular hyperplasia and formation of whorl structures of the endoplasmatic reticulum together with the presence of exocytosis vesicles on the cell surface may be the morphological manifestation of endocrine activity of the tumor. The identity of ultrastructural and optical diffraction characteristics of the crystal inclusions in both cells (hilar and testicular interstitial) favours the assumption of an homology of both cells and their neoplasms.     

抗人卵巢癌×抗人CD3×抗CD28VH单链三特异抗体的胞内可溶表达、纯化及体外活性测定

目的　研究抗人卵巢癌 (ovariancarcinoma ,oc)×抗人CD3×抗CD2 8VH 单链三特异抗体(singlechaintrispecificantibody,scTsAb)在大肠杆菌中的可溶表达与纯化及纯化后产物的活性测定 ,从而为其应用于卵巢癌治疗的临床研究打下基础。方法　将已构建的scTsAb表达载体转化大肠杆菌BL2 1(DE3)Star菌株 ,采用低温 (30℃ )、低剂量IPTG(0 .2mmol L)诱导 ,进行胞内可溶表达。根据抗卵巢癌三特异抗体 (ocTsAb)等电点较高 (pI9.0 ) ,而菌体蛋白大多为酸性蛋白的特点 ,利用DEAE弱阴离子交换层析(pH8.0 )进行一步纯化 ,并利用ELISA及FACS的方法检测纯化后抗卵巢癌三特异抗体的活性。结果　(1)SDS PAGE鉴定低温诱导时可溶比例达到 5 6 %。 (2 )绝大多数菌体蛋白被DEAE层析柱吸附 ,而抗卵巢癌三特异抗体在穿透液中流出 ,SDS PAGE检测纯度达到 90 %。 (3)ELISA结果显示纯化后的抗卵巢癌三特异抗体与重组CD2 8纯抗原 ,Jurkat(CD3 )细胞膜提取抗原 ,SKOV3细胞膜提取抗原均有特异性结合。 (4 )FACS结果证明纯化后的抗卵巢癌三特异抗体与Jurkat(CD3 )活细胞、SKOV3活细胞有特异性结合。结论　低温诱导胞内可溶表达的抗人卵巢癌×抗人CD3×抗CD2 8VH 单链三特异抗体经弱阴离子交换层析一步纯化后仍保持原有免疫学活性 ,这     

HEREDITARY HEMORRHAGIC TELANGIECTASIA WITH MALIGNANT LYMPHOMA An Autopsy Case

A 60-year-old Japanese woman was diagnosed at autopsy as having had hereditary hemorrhagic telangiectasia (HHT) associated with systemic hemangiomas. In her repoduction period, premenstrual epistaxis frequently occurred. At the age of 60, the patient died of malignant lymphoma. At autopsy, multiple telangiectatic spots were noted on the face, limbs and trunk. The paraaortic lymph nodes, which were enlarged and irregularly conglomerated, were histologically diagnosed as malignant lymphoma of the diffuse large cell type. Submucosal telangiectatic lesions were found in the gastrointestinal system from the oral cavity to the rectum. Cavernous hemangiomas were present in various visceral organs including the liver, spleen, small and large intestines, rectum, appendix, uterus, and jejunal and colonic mesenteries. There was an arteriovenous fistula in the left lung. Examination of her family pedigree showed that the patient had an autosomal dominant trait of inheritance. The pathogenesis of the systemic visceral hemangiomas observed in this patient was considered to be similar to that of hamartoma.     

血清CA125测定对卵巢恶性肿瘤的诊断及随访复发的价值

本文采用放射免疫法对９８例６１５份血清进行糖类抗原ＣＡ１２５值的测定，其中正常对照３０例，卵巢良性肿瘤２５例，交界性肿瘤１例，卵巢恶性肿瘤４２例。对恶性肿瘤患者中２４例同时进行了阴道超声检查的随访监测，发现卵巢恶性是血清ＣＡ１２５值明显高于良性肿瘤和正常对照组（Ｐ〈０．０１）；良性肿瘤ＣＡ１２５值与正常对照组无显著性差异（Ｐ〉０．０５）。术前卵巢恶性肿瘤阳性检出率８５．１９％，其中卵巢上皮性癌阳性