Plasma membrane-mediated nuclear uptake and chromatin binding of insulin in tumor cell lines

Analysis of different cellular fractions after incubation of SW 948 and SW 707 colorectal carcinoma cells or WM 266-4 melanoma cells with 125I-insulin revealed the nondegraded hormone in the chromatin of these cells. Nuclear 125I-insulin was bound to specific fragments of EcoRI-, HaeIII-, and HincII-digested chromatin. A 45-kDa chromatin protein species that binds 125I-insulin was identified. Actinomycin D and cycloheximide inhibited the insulin-stimulated expression of chromatin receptors. Uptake of 125I-insulin by isolated nuclei occurred only in the presence of plasma membranes. Thus, at least some effects of insulin on target cells can be explained by direct gene regulation instead of "second messenger" action.     

冷冻浓缩腹水回输在治疗顽固性肝硬化腹水中的应用

目的 探讨冷冻浓缩腹水回输治疗顽固性肝硬化腹水的疗效。方法 采用透析机对32例顽固性肝硬化腹水患者的腹水进行超滤浓缩,置-18℃环境中冷冻2～3天,37℃温育溶解后静脉回输。结果 冷冻浓缩腹水回输前后,血清总蛋白为(59.1±5.6)g/L和(66.3±4.7)g/L,白/球比值为0.8±0.3和1.1±0.5,腹水总蛋白为(15.2±6.8)g/L和(55.6±8.4)g/L;腹围为(91.3±9.5)cm和(80.8±9.2)cm,腹压为(3.5±0.43)kPa和(2.4±0.32)kPa,24 h尿量为(615.0±185.5)ml和(1 240.0±255.0)ml;治疗前后相比,差异有高度显著性(P<0.01)。血清钾为(3.5±1.1)mmol/L和(4.0±1.3)mmol/L,腹水钾(3.6±0.9)mmol/L和(4.1±1.0)mmol/L;血清钠为(122.2±4.0)mmol/L和(126.5±4.3)mmol/L,腹水钠为(120.8±4.5)mmol/L和(123.3±4.7)mmol/L;血清氯为(90.3±6.2)mmol/L和(95.1±5.7)mmol/L,腹水氯为(92.0±5.9)mmol/L和(96.4±50.5)mmol/L;治疗前后相比,血清钠、腹水钠、腹水钾、血清氯、腹水氯差异均有显著性(P<0.05,P<0.01),血清钾差异无显著性(P>0.05)。冷冻浓缩腹水回输后,除2例出现一过性发热外,未发生低血压、肝昏迷、消化道出血现象,未出现蛋白凝集、堵塞管腔;未出现电解质紊乱现象。结论 冷冻浓缩腹水回输可大量回收腹水     

5-氨基水杨酸片剂的抗炎镇痛作用

目的　研究 5 -氨基水杨酸 (5 ASA)的主要药效学。方法　镇痛试验采用小鼠热板法及扭体法 ;抗炎试验采用小鼠耳片法、蛋清诱导大鼠足肿胀模型。结果　 5 ASA片剂 3种剂量 (75、15 0及 30 0mg·kg-1,ig× 3d)对二甲苯诱导的小鼠耳肿胀度及蛋清诱导的大鼠踝关节肿胀度均有明显抑制作用 ;以上剂量还可明显抑制小鼠扭体反应 ,大剂量还可延长小鼠热板的舔足反应潜伏期。结论　 5 ASA片剂有明显的抗炎镇痛作用。     

英汉颜色词差异浅探

英、汉语分属两种不同的语系 ,受语言与文化特殊性的影响 ,人们对色彩的感受和赋予颜色词的意义也各不相同。翻译时 ,不可望文生义 ,“对号入座” ,必须在充分了解中西方文化差异的基础上 ,准确把握颜色词所处的语境 ,力求做到信、达、雅     

子宫腺肌病误诊原因分析及术前诊断临床探讨

目的;探讨子宫腺肌病误诊原因及近年术前诊断研究进展,方法;对我院近１０年收治的１９７例腺肌病患者的临床资料进行回顾性分析。结果：１９９０年元月至１９９７年１２月１２８例腺肌病患者术前,术后诊断符合率仅为２９．７％,术后误诊,漏诊率达７０．３％,１９９８年元月至１９９９年１２月６９例术前经测定血清ＣＡ１２５及 腔双氧水造影后误诊,漏诊率降为２７．５％术前主要误诊为子宫肌瘤或（和）子宫内膜异位症,结论     

The effect of TGFβ1 on murine tumor growth following direct intratumoral injection of plasmid DNA

In order to investigate TGFβ1 gene expression and its effect on murine tumor growth following direct intratumoral injection of naked plasmid DNA encoding human TGFβ1, LM₃ murine lung adenocarcinoma cells were inoculated subcutaneously to T₇₃₉ mice and grew to tumor nodules in 2 weeks. Multiple direct intratumoral injection of plasmid DNA, PMAMneo- TGFβ1, were given and compared with saline or vector plasmid administration groups. The growth of tumor was observed till the 8th week when the mice were killed for Northern blot analysis and histopathological study of tumoral tissue. The results showed that the growth of tumor was boosted in the TGFβ1 gene treated mice as compared with the control groups, whereas there was no significant difference in the metastatic behavior. Northern blot showed efficient expression of TGFβ1 mRNA in the treated group. The present study indicated that TGFβ1 may stimulate tumor growthin vivo through certain mechanisms. And direct intra-tumoral injection of nude plasmid DNA may be a promising gene transfer strategyin vivo. This work was supported by the National Natural Science Foundation of China (No. 39370761).     

Bone scan of hemodialysis patients with secondary hyperparathyroidism before and after parathyroidectomy

Renal osteodystrophy (ROD) accompanied by long-term hemodialysis patients with chronic renal failure includes several forms of disorders of mineral and skeletal metabolism such as osteitis fibrosa attributed to secondary hyperparathyroidism, osteomalasia and adynamic bone disease. Bone scan is performed to detect of the mainly pathophysiology of ROD. We investigated bone scan of 25 hemodialysis patients with secondary hyperparathyroidism diagnosed clinically before and after parathyroidectomy (PTX). Before PTX an diffusely high accumulation of bone seeking agent in the whole skeleton especially skull in all patients (100%), vertebra in 24 out of 25 (96%), patella in 24/25 (96%), limbs in 23/25 (92%), sternum in 19/25 (76%), sacrum in 18/25 (72%) and costochondral junctions in 14/25 (56%) was noted in these patients. The radionuclide activity of the calvaria, maxilla and mandible in the skull was prominently high. Fourteen patients had an equally high activity in the calvaria, maxilla and mandible, 6 patients had higher activity in the maxilla and mandible than that of calvaria and 5 patients had higher in the calvaria than that of maxilla and mandible. After PTX the changes in the skull were obvious in 19 patients who showed a more markedly decreased in activity of the maxilla and mandible than that of the calvaria. In 3 patients showed a more markedly decreased in activity of the calvaria than that of the maxilla and mandible. Another 3 demonstrated equally decreased in activity in the calvaria, maxilla and mandible. It became clear that the highest activity of the skull was shown in all patients and the therapeutic changes of the skull are the most pronounced in maxilla and mandible in this study.     

试论当前无偿献血工作的几个关键环节

在宣传贯彻落实《献血法》,开展公民无偿献血工作启动阶段,有许多工作要做,其中有五项是关键性的,即制定具体实施细则,建立管理机构网络,广泛宣传发动,分配献血指标和提供便利的献血条件。抓好这五个关键环节,无偿献血工作才能较好地开展下去。     

若干疾病中医证的流行病学研究进展

以流行病学在病证结合的中医“证”研究中的应用为线索，光盘检索和手工查阅了1997—2002年的期刊文献，对若干疾病运用流行病学方法进行的大样本(100例以上)的中医证型临床研究结果作一个回顾和小结，从流行病学和统计学的角度分析科研和论文中存在的问题，提出改进方法和展望。