Role of intestinal bifidobacteria in the pathogenesis of gut-derived bacteria/endotoxin translocation and the effect of bifidobacterial supplement on gut barrier function following burns

Early multiple organ dysfunction syndrome appears to be facilitated with bacterial translocation in severely burn injury, yet the mechanisms of bacterial translocation remains in dispute. The aim of this study was to investigate the potential role of intestinal bifidobacteria in the pathogenesis of gut-derived bacteria/endotoxin translocation following burns and the effects of bifidobacterial supplement on gut barrier. Methods: Wistar rats were randomly divided into burn group (Burn, n=60),sham burn group (SB, n=10) in experiment Ⅰ , and burn + saline group (BS, n=30), burn + bifidobacteria group (BB, n=30), and sham-burn + saline group (SS, n= 10) in experiment Ⅱ. Animals in BB group were fed bifidobacterial preparation (5 × 109 CFU/ml) after burns, 1.5ml,twice daily. Animals in BS and SS were fed saline. Samples were taken on days 1, 3, and 5 in burn groups, and on day 3 in sham-burn groups. The incidence of bacteria/endotoxin translocation and counts of Bifidobacterium, Fungi and Escherichia coli in gut mucosa were determined with standard methods. The levels of sIgA in mucus of small intestine were measured by RIA. The positive sIgA expression in lamina propria and ileum mucosal injury was evaluated light microscopically by blinded examiners. Results: Our results showed that the incidence of bacterial translocation was increased after burns, which was accompanied by significant decrease in number of bifidobacteria but significant increase in E. coli and fungi in gut mucosa, and elevation of levels of plasma endotoxin and IL-6 (P<0. 001).The incidence of bacterial translocation was markedly reduced after 3- and 5-day supplementation of bifidobacteria compared with control group (P<0.05). The counts of mucosal bifidobacteria were increased by 4- to 40-fold,while E. coli and fungi were decreased by 2- to 30-fold and 10- to 150-fold, respectively, after bifidobacterial supplementation in contrast to control group. The damage of mucosa tended to be less pronounced after 3-day bifidobacteria-supplemented formula compared with control group [grade 2(0-6) vs. grade 4(3-6), P<0.05]. Moreover, the expression and release of sIgA was markedly augmented after 3-day bifidobacteria-supplementation formula and it returned to normal range on day 5. Conclusion: The decrease in counts and proportion of bifidobacteria in mucous membrane flora may play an important role in the development of bacteria/endotoxin translocation following thermal injury. The supplement of exogenous bifidobacteria could per se improve gut barriers, and attenuate bacteria/endotoxin translocation secondary to major burns.     

栀子提取液对大鼠急性胰腺炎时TNF-α和IL-6改变的影响

目的：探讨急性胰腺炎时血清TNF-α和IL-6的变化以及栀子提取液对其的影响。方法：SD大鼠造模后口服给予栀子提取液进行药物治疗，24h后呆血测淀粉酶、TNF-α和IL-6，并分组对比，取胰腺组织做病理学检查。结果：急性胰腺炎时血淀粉酶、TNF-α和IL-6水平显著升高，镜下见大量炎细胞浸润，栀子提取液可改善上述变化。结论：栀子提取液对大鼠急性胰腺炎有治疗作用。     

IL-2和IL-4联合诱导B细胞死亡受体CCR3的表达及其功能研究

目的　研究在IL 2和IL 4作用下 ,趋化性细胞因子受体CCR3在人生发中心 (germinalcenter,GC)B细胞上的表达及其功能特性。方法　采用流式细胞术检测人GCB细胞上CCR3表达和在CCR3配体eotaxin作用下B细胞的凋亡 ,实时定量RT PCR和Northernblot法检测GCB细胞内CCR3mRNA的表达 ,淋巴细胞趋化和黏附试验检测B细胞的趋化和黏附能力。结果　人GCB细胞极低表达趋化性细胞因子受体CCR3,经IL 2和IL 4作用后 ,GCB细胞高表达CCR3,但此时CCR3不能在其配体作用下诱导GCB细胞的趋化和黏附功能 ,而是诱导GCB细胞凋亡。结论　IL 2和IL 4联合诱导人GCB细胞CCR3表达 ,CCR3可能具有死亡受体的功能。     

原位杂交法检测胃癌组织中IL-6及其受体mRNA的表达

胃癌是最常见的癌肿之一,应用原位杂交技术检测胃癌组织中IL—6及其受体mRNA的表达情况,具有灵敏度高、特异性强的特点,而且较直观地在组织原位和转录水平对标本中IL—6及其受体进行研究,这使得形态学观察与功能学检测相结合,更能反映胃粘膜不同病变细胞分泌IL—6及其受体的情况。     

P27 Cyclin D_1在胆囊癌中的表达及意义

目的研究p2 7、CyelinDl 在胆囊癌中的表达情况 ,并探讨它们与肿瘤分化程度及生物学行为的关系。方法应用免疫组织化学S -P法检测 10例胆囊炎及 32例胆囊癌标本p2 7及CyelinDl的表达状况。结果p2 7在胆囊炎上皮中为低表达 ,在胆囊癌中少部分表现出高表达。而CyelinDl 在胆囊炎上皮中低表达 ,而在胆囊癌中大部分呈现高表达 ,两者间差异显著 (P <0 .0 5 )。结论CyclinDl在胆囊癌的发生发展中有重要作用 ,且与患者预后有关。     

保肾口服液影响IgA肾病小鼠T细胞增殖及分泌IL-2的实验研究

观察保肾口服液对IgA肾病小鼠T细胞^3H-TdR掺入及其分泌IL-2水平的影响，结果表明低、中、高3种浓度的保肾口服液均能显著促进IgA肾病小鼠的自发性和ConA刺激的T细胞^3H-TdR掺入，促进T细胞合成，分泌IL-2，提前给药作用相同。提示保肾口服液有显著增强IgA肾病小鼠细胞免疫功能的作用。     

大疱及疱疹性皮肤病

20060331天疱疮患者外周血单一核细胞IL-6受体TNFa受体mRA的表达/钟连生(徐州医学院附院皮肤科),马道铭,崔盘根…∥中国麻风皮肤病杂志.-2005,21(9).-673~675用逆转录-聚合酶链反应法对37例寻常型天疱疮患者及30例健康者外周血单一核细胞(PBMCS)上IL-6受体爱肿瘤坏死因子α(TNF     

提高撰写医学论文英文摘要的水平

英文摘要(abstract)英文(abstract)一词作为名词其含义是shortaccount of the chief points of a piece of writing(一篇文章中要点的简写),作为形容词其反义词是实质性(concrete),作为名词其反义词是正文(text)。Ab-stract有几个近义词:summary(总结、小结)和conclu-sion(结论