罗格列酮对多囊肾囊肿衬里上皮细胞p38促分裂原活化蛋白激酶信号通路的影响

Objective To investigate the effect of rosiglitazone on p38 mitogen-activated protein kinase (p38MAPK) pathway in polycystic kidney cyst-lining epithelial cells. Methods The cyst-lining epithelial cells (PKD cells) from human polycystic kidney were treated with rosiglitazone (10 μmol/L), peroxisome proliferator-activated receptor-γ (PPARγ) inhibitor GW9662 (10 μmol/L), rosiglitazone (10 μmol/L) +GW9662 (10 μmol/L), p38MAPK specific inhibitor SB203580 (10 μmol/L), SB203580 (10 μmol/L)+ rosiglitazone(10 μmol/L) for 2 hours followed by epidermal growth factor (EGF) stimulation. Protein expressions of p38, phuspho-p38 (p-p38) and proliferating cell nuclear antigen (PCNA) were detected by Western blot. p38 mRNA was examined by RT-PCR. Expression of c-fos and c-jun was observed by immunocytochemistry. Results (1) EGF markedly up-regulated the expressions of p38, p-p38, PCNA, c-fos anti c-jun compared with control group (P<0.01). (2) Compared with EGF treated group, rosiglitazone significantly reduced p38 activation and mRNA expression (P<0.01, respectively). Rosiglitazone, rosiglitazone+SB203580 could significantly down-regulated p-p38, PCNA, c-fos and c-jun expression (P<0.01, respectively) with no significant difference between these two groups. (3) GW9662 partially reversed the reduction effect of rosiglitazone. Conclusions Rosiglitazone can inhibit proliferation of autosomal dominant polycystic kidney disease cyst-lining epithelial cells partially through down-regulating p38 activation and reducing c-fos, c-jun and PCNA expression. The above effect of rosiglitazone is in part PPARγ-independcnt.     

激光加放射治疗菜花型宫颈癌6例报告

激光加放射治疗菜花型宫颈癌６例报告山东省肿瘤医院（济南市２５０１１７）梁焕玲，佟锦职，候晓彤我院１９９３年１１月～１９９４年４月收治巨大莱花型宫颈癌６例，采用激光加放射治疗，疗效显著，报告如下。１资料与方法１．１一般资料：６例经组织学诊断为宫颈鳞癌（...     

前激肽释放酶（PK）含量测定临床应用的初步探讨

前激肽释放酶（ＰＫ）含量测定临床应用的初步探讨福建省立医院林惠，黄秀琼福建医学院附属协和医院陈卫闽，罗心绮血浆前激肽释放酶ＰＫ是血浆激肽释放酶Ｋ的前体，由肝脏合成和分泌的，是凝血系统与凝血因子密切相关的蛋白质，在凝血的接触系统中占重要地位，故检测血浆...     

肩关节周围炎综合治疗的护理

<正> 肩关节周围炎是由于肩关节及其周围软组织因退行性改变、劳损等,引起的肩部及关节活动受限骨关节的无菌性炎症,包括粘连性关节炎、滑囊炎、肱二头肌长头腱鞘炎及肩袖炎等疾病,对其治疗大多采用痛点注射或小针刀治疗。我院则采用痛点注射和手法松解综合治疗,临床疗效满意,现将其护理报告如下。 1 资料与方法 1.1 一般资料 150例,男83例,女67例。年龄52～60岁,初诊肩周炎确立,经X线照片排除颈椎病和其它疾病后,列     

CT导向血肿穿刺吸除加尿激酶冲洗治疗高血压脑出血

目的 探讨CT导向下行血肿穿刺吸除并用尿激酶冲洗治疗高血压脑出血的临床效果。方法 经CT扫描，确定穿刺点、穿刺方向及深度，穿刺吸除大部分血肿，术后向血肿腔注入尿激酶冲洗、引流。结果 168例患者中恢复良好51例，轻残61例，重残29例，死亡27例，死亡率16．1％，效果良好。结论 CT导向下血肿穿刺抽吸加尿激酶冲洗，有利于充分清除血肿，创伤小、操作简单易行，特别适合设备相对简单的基层医院。     

心肌可激媒质中一对螺旋波的碰撞涅灭的数值实验研究

利用FHN模型，我们研究了二维心肌可激媒质系统中一对螺旋波，在均匀的周期小电流驱动下，碰撞湮灭条件。发现：(1)驱动电流的初位相决定螺旋波漂移初始方向；两个螺旋波靠近时，它们之间引起排斥作用；(2)在较大的强度驱动时，适当的位相条件下，一对反向的螺旋波将被驱除边界或碰撞湮灭；(3)在共振驱动时，一对反向的螺旋波较易碰撞湮灭。