Convergence from the preoptic area and arcuate nucleus to the median eminence in acupuncture and nonacupuncture point stimulation analgesia

Lesion of the preoptic area (POA) or medial arcuate nucleus (M-HARN) abolished acupuncture analgesia (AA). Potentials in the median eminence (ME) evoked by stimulation of the acupuncture point (AP) were not affected by lesion of either the POA or M-HARN alone, but were abolished by concurrent lesion of both. No analgesia was produced by stimulation of the POA. Analgesia produced by stimulation of the M-HARN was abolished by lesion of the POA, and the abolished analgesia was restored by concurrent stimulation of the POA and M-HARN, hence POA and M-HARN outputs might converge in the ME to produce AA. Similar convergence from the anterior arcuate nucleus (A-HARN) and POA to the ME was observed in analgesia (NAA) produced by stimulation of a nonacupuncture point (NAP). Two pathways diverged from the lateral hypothalamus in the AA afferent pathway and two from the lateral periaqueductal central gray (L-PAG) in the NAA afferent pathway. POA potentials evoked by stimulation of the AP were reversed by naloxone, and those evoked by stimulation of the AP were reversed by dexamethasone. ACTH sensitive sites were found in both the L-PAG and the anterior hypothalamus.     

高血压病患者血清抗核抗体的检测与分析

本文报道了用酶联A蛋白的ELISA法检测高血压病患者血清抗核抗体的结果。检测40例高血压病患者和60份正常人血清。结果显示,高血压病患者特别是较重病例其血清抗核抗体有较高的阳性率,与正常人比较差异显著。提示高血压病与免疫特别是与自身免疫有一定关系。有助于高血压病病因及发病机理的探讨。     

电刺激大鼠下丘脑室旁核对脊髓背角神经元伤害感受性反应的影响

麻醉或制动大鼠,用玻璃微电极记录脊髓背角伤害性单位活动及其对电刺激下丘脑室旁核(PVN)的反应,并观察了电解中脑导水管周围灰质(PAG)和中缝大核(NRM)后伤害反应的变化。实验结果:①电刺激PVN能明显抑制脊髓背角对刺激坐骨神经所诱发的伤害性反应,其作用在刺激PVN 3min时最为显著。②分别电解PAG和NRM后,PVN抑制伤害反应的作用仍存在。结果提示PVN参与了脊髓水平伤害信息的调制,而这种作用未经PAG和NRM的中继。     

Laparo-endoscopic single site cholecystectomy versus standard laparoscopic cholecystectomy: results of a pilot randomized trial

Background

In recent years, new devices providing multiple channels have made the performance of laparoscopic cholecystectomy through a single access site not only feasible but much easier. The potential benefits of laparoendoscopic single-site (LESS) cholecystectomy may include scarless surgery, reduced postoperative pain, reduced postoperative length of stay, and improved postoperative quality of life. There are no comparative data between LESS cholecystectomy and standard laparoscopic cholecystectomy (LC) available at present with which to quantify these benefits.

Methods

This study was a prospective, randomized, dual-institutional pilot trial comparing LESS cholecystectomy with standard LC. The primary end point was postoperative quality of life, measured as length of hospital stay, postoperative pain, cosmetic results, and SF-36 questionnaire scores. Secondary end points included operative time, conversion to standard LC, difficulty of exposure, difficulty of dissection, and complication rate.

Results

No significant differences in postoperative lengths of stay were found in the two groups. Postoperative pain evaluation using a visual analogue scale showed significantly better outcomes in the standard LC arm on the same day of surgery (P = .041). No differences in postoperative pain were found at the next visual analogue scale evaluation or in the postoperative administration of pain-relieving medications. Cosmetic satisfaction was significantly higher in the LESS group at 1-month follow-up (mean, 94.5 ± 9.4% vs 86 ± 22.3%; median, 100% vs 90%; P = .025). Among the 8 scales of the SF-36 assessing patients' physical and mental health, scores on the Role Emotional scale were significantly better in the LESS group (mean, 80.05 ± 29.42 vs 68.33 ± 25.31; median, 100 vs 66.67; P < .0001).

Conclusions

In this pilot trial, LESS cholecystectomy resulted in similar lengths of stay and improved cosmetic results and SF-36 Role Emotional scores but performed less well on pain immediately after surgery. A larger multicenter trial is needed to confirm and further investigate these results.     

Production and Characterization of Monoclonal Antibodies against Bovine Luteinizing Hormone

Five mouse hybridoma cell lines producing monoclonal antibody against bovine luteinizing hormone (LH) have been established and the respective antibodies characterized by radioimmunoassay, immunofluorescence and immunoelectrophoresis. All antibodies belong to the IgG class and bind to staphylococcus protein A. Intraspecies cross-reactivity studies revealed no reaction with bovine follicle stimulating hormone (FSH). However, all antibodies showed partial cross-reaction with bovine thyroid stimulating hormone (TSH) suggesting a close conformational similarity between bovine LH and TSH. Studies on interspecies cross-reactivity (rat and human) showed that three of these five antibodies strongly react with rat LH but not at all with either rat FSH or rat TSH thus representing monospecific reagents for investigations concerning LH in this species. One of these three antibodies also strongly binds to human LH and to the same extent to human chorionic gonadotropin (CG) but not to human FSH or TSH. It was concluded that at least three different epitopes on the bovine LH molecule are recognized and that they are located on the β-chain of the hormone.     

基于SPA技术的网络随访系统的设计与实现

目的：设计和实现具有随访数据录入/检索/导出、历史随访记录对比、疾病风险量表评估等功能的网络随访系统,使用户可以随时随地管理和分析患者随访数据。方法：基于目前较为流行的MEVN全栈开发架构,设计与实现了界面简洁、功能完善的随访系统SPA,并将其部署到云服务上。结果：成功地完成了基于SPA技术的网络随访系统,在临床科室取得了较好的应用,得到了医护人员的一致肯定。结论：采用MEVN架构的SPA技术具有开发效率高、适应复杂数据模型、并发性能好、易部署等特点。基于SPA技术研发的网络随访系统能有效提高随访效率,改善科研环境。     

TTF-1和SPA表达对早期肺腺癌术后复发预测及预后的判断价值

目的 研究术后肺腺癌患者组织中TTF-1和SPA的蛋白表达对其复发的预测价值及预后的影响.方法 收集行手术切除的Ⅰ～Ⅲ期肺腺癌患者112例,采用免疫组化方法检测组织中TTF-1以及SPA的表达.结果 68例患者出现复发,TTF-1阳性和阴性表达患者中位DFS分别为41个月和9个月(P =0.013),中位OS分别为65.0和23.0个月(P=0.037),SPA阳性与阴性表达患者中位DFS分别为59.0个月和15.0个月(P=0.018),中位OS分别为68.0个月和35.0个月(P =0.058).结论 早期肺腺癌术后组织中TTF-1和SPA的表达是其复发的有效预测指标,TTF-1阳性表达预后更好.     

悬吊式单切口腔镜手术治疗男性乳腺发育症

目的探讨悬吊法单切口腔镜手术治疗男性乳腺发育症的临床价值。方法 2007年12月～2009年6月对11例男性乳腺发育症采用悬吊法行皮下单纯乳腺切除术,在隐蔽部位做2cm小切口,利用SPA器械完成手术。结果 11例手术均获成功,手术时间105～145min,平均122min。术后住院3～10d,平均5.3d。11例随访2～20个月,平均12个月,外形均较满意,乳头形态正常。结论单切口悬吊法腔镜下乳腺切除术有较好的美容效果且手术安全。