分泌抗SPA单克隆抗体杂交瘤细胞系的建立与鉴定

以 SPA 作基础免疫 BALB/C 小鼠后,融合前三天直接脾内免疫,取脾细胞与 SP_2/O 瘤细胞常规融合,融合率100%;以快速 ELISA 法检测抗体,阳性率60%。5次有限稀释法克隆化后获得7株杂交瘤细胞,作中和抑制试验,证实为 SPA 特异性抗体。经鉴定属 IgG2a 及 IgG2b 亚类。电镜观察细胞融合,发现其细胞内含逆转录病毒。杂交瘤细胞液氮保存与体外连续传代一年,仍保持原有各种特性。     

中国人骨质疏松症诊断标准回顾性研究

目的　通过对既往国内文献复习 ,提出一种更方便、更科学的中国人骨质疏松症诊断标准。方法　检索国内期刊数据库中已发表的文献 ,摘录骨密度丢失率数据进行统计、计算、分析 ,得到不同年龄段、不同作者、使用不同型号仪器、不同部位的骨密度丢失百分率及相关曲线。结果　中国男性骨质疏松诊断标准推荐使用骨密度丢失百分率 2 5 %或 2SD ;中国女性骨质疏松诊断标准推荐使用骨密度丢失百分率 2 5 %或 2SD ;测量部位推荐使用如下顺序 :股骨颈 >前臂远端 1/ 3、1/ 10、1/ 6 >RA法手指骨 2 ,3,4中节 >股骨Troch区 ;不推荐使用股骨Ward区和腰椎侧位。结论　使用骨密度丢失百分率这个指标作为中国人骨质疏松症诊断标准非常有意义 ,值得进一步推广和深入研究。     

Periaqueductal gray stimulation: Effect on characterized nucleus gigantocellularis neurons in the rat

Although stimulation of the periaqueductal gray (PAG) and microinjection of opiates or opioids into the PAG was reported to affect the activity of nucleus gigantocellularis (NGC) neurons, it is not known whether a direct pathway exists between these brain regions. The purpose of this investigation was to electrophysiologically test this possibility by attempting to antidromically activate PAG neurons from the NGC. Seventy neurons in the PAG of urethane-anesthetized rats were activated by electrical stimulation in the NGC and classified on the basis of the antidromicity criteria of constant latency, high following frequency, and collision. Of these neurons, 50 (71%) were classified as being transsynaptically activated, and 20 (29%) were classified as being antidromically activated. Ten neurons demonstrated collision between spontaneous action potentials and stimulation-evoked action potentials. Although most of the responses could not be tested with all criteria, the data support the existence of a monosynaptic pathway from the PAG to the NGC.     

Single Port Access (SPA) Splenectomy

Introduction:

Over the last decade, laparoscopic splenectomy has become the standard of care for spleen removal. Elimination of a large incision and difficult exposure has decreased postoperative morbidity and length of stay. Single port access (SPA) surgery was developed as an alternative to traditional multiport laparoscopy, potentially exploiting the already proven benefits of minimally invasive surgery. We apply the SPA technique to splenectomy via a single umbilical incision.

Methods:

SPA splenectomy was performed in a 36-year-old male for staging. The single-port access technique was used to gain abdominal entry. Exposure, dissection, and removal were performed via a single incision within the umbilicus. The final incision was extended for removal of a complete specimen for pathologic evaluation.

Results:

Splenic mobilization and control of the short gastrics was successfully performed via a single umbilical incision. The final incision was extended inferiorly for en bloc organ removal. Follow-up at 18 months revealed a well-healed incision with no signs of hernia formation.

Conclusions:

The single-port access technique has been successfully applied to splenectomy as an available alternative to multiport laparoscopic splenectomy. Use of standard instrumentation and trocars maintains costs and familiarity of the procedure. Exposure, visualization, and dissection are the same as in standard laparoscopy. SPA surgery may be more ergonomically pleasing to the surgeon and offer patient benefits, such as faster recovery and decreased adhesion formation in the long term.     

Oncological long-term outcome of 4772 patients with prostate cancer undergoing radical prostatectomy: Does the anaesthetic technique matter?

IntroductionRecent data suggest that using additional neuroaxial anaesthesia during oncological surgery is associated with favourable recurrence-free survival, when compared with general anaesthesia alone. We assessed the impact of adjunctive perioperative spinal anaesthesia and dose of opioids on the oncological long-term outcome of patients following radical prostatectomy.MethodsWe selected patients from our institutional review board-approved database who consecutively underwent radical prostatectomy between 2002 and 2007. Patients were stratified by type of anaesthesia, administered as general anaesthesia alone, or spinal anaesthesia in addition to general anaesthesia. Biochemical recurrence-free survival, metastasis-free survival and overall survival were analysed by a multivariate Cox regression model and by Kaplan–Meier analysis in propensity-score based matched cohorts, adjusted for standard clinico-pathological variables and year of surgery.ResultsOverall, 4772 patients were analysed. Regarding the type of anaesthesia no significant difference for biochemical recurrence-free survival, metastasis-free survival and overall survival was analysed by a multivariate Cox regression model (p = 0.5, 0.8 and 0.7). The Kaplan–Meier analyses after propensity-score matched based comparisons revealed no significant difference depending on type of anaesthesia for biochemical recurrence-free survival, metastasis-free survival and overall survival (p = 0.6, 0.1 and 0.4). The same accounted for a propensity-score matched model adjusted for the year of surgery on biochemical recurrence-free survival (p = 0.7).ConclusionsThe oncological outcome after radical prostatectomy was not affected by the adjunctive use of spinal anaesthesia.     

Blue-light-dependent interaction of cryptochrome 1 with SPA1 defines a dynamic signaling mechanism

Cryptochromes (CRYs) are blue-light photoreceptors that mediate various light responses in plants and animals. The signaling mechanism by which CRYs regulate light responses involves their physical interactions with COP1. Here, we report that CRY1 interacts physically with SPA1 in a blue-light-dependent manner. SPA acts genetically downstream from CRYs to regulate light-controlled development. Blue-light activation of CRY1 attenuates the association of COP1 with SPA1 in both yeast and plant cells. These results indicate that the blue-light-triggered CRY1-SPA1 interaction may negatively regulate COP1, at least in part, by promoting the dissociation of COP1 from SPA1. This interaction and consequent dissociation define a dynamic photosensory signaling mechanism.     

A Study of Complete Superficial Palmar Arches Formed Entirely by Ulnar Artery

Superficial Palmar Arch is an arterial arcade and a dominant vascular structure in the palm. It is defined as the anastomosis between the superficial branch of the ulnar artery and supericial palmar branch of the radial artery. In ulnar dominant complete superficial palmar arch, the ulnar artery does not anastomose with radial artery and it terminates by supplying thumb and index finger. In the present study, this type of variation was found in 50% of the hands. This is in contrast to the classical superficial palmar arch normally described where the arch is completed on the radial side by superficial palmar branch of radial artery. In accordance with the present study, a feature that is present in 50% of the specimens cannot be called as a variation. In these cases, without an efficient collateral circulation, ulnar artery occlusion may cause claudication and gangrene in the digits and has clinical significance.     

胶体金免疫层析检测犬、猫抗狂犬病病毒IgG抗体的研究

目的建立检测犬、猫抗狂犬病病毒(RV)IgG抗体的胶体金免疫层析方法。方法采用柠檬酸三钠还原法制备胶体金用以标记SPA,同时将重组RVN蛋白、抗SPA抗体分别包被至硝酸纤维素膜的检测线与质控线上,制备一种检测犬、猫抗RVIgG抗体的胶体金检测卡,进行了特异性和灵敏度试验,并与ELISA同时检测临床样品、统计结果。结果 GICA试纸条检测灵敏度为0.5IU/mL,与犬瘟热病毒、犬细小病毒等阳性血清无交叉反应,并与ELISA相比,两者的符合率为94.6%。结论成功建立了检测犬、猫抗RVIgG抗体的通用型胶体金免疫层析方法 ,该方法灵敏度高,特异性强,检测速度快,操作简便,可广泛应用于基层。