外剥内扎硬注加辅助切口术治疗环状混合痔的临床观察

目的探讨开放性痔外剥内扎硬注加辅助切口的方式治疗环状混合痔的临床效果。方法将我院2000年1月至2008年12月收治的120例环状混合痔,选取具有可比性的观察组（外剥内扎硬注加辅助切口术治疗）和对照组（外切内扎硬注术治疗）各60例,在疗效、疗程、并发症、复发、后遗症等方面作对比分析。结果观察组总有效率100%;平均疗程（12.96±3.61）d;术后并发症1例（出血、水肿）,随访2年复发2例。对照组总有效88.33%,平均疗程（16.23±4.49）d;术后并发症（出血、水肿）22例;随访2年复发12例。结论观察组明显优于对照组（P〈0.01）。外剥内扎硬注加辅助切口术治疗环状混合痔疗效确切、复发率低、术后并发症少,是治疗环状混合痔较好的方法。     

菱形切口外剥内扎注射术治疗混合痔100例临床研究

目的研究菱形切口外剥内扎注射术治疗混合痔的临床价值。方法将200例混合痔患者随机的分为两组,每组100例,菱形切口外剥内扎注射术为治疗组和对照组外剥内扎术进行对比观察。结果治疗组在术后继发出血,术后尿潴留及疗效方面与对照组比较无统计学意义（P〉0.05）,但在术后疼痛、术后肛门功能影响、伤口愈合时间、复发等方面比较有统计学意义（P〈0.05）。结论菱形切口外剥内扎注射术治疗混合痔在术后疼痛情况、术后肛门功能影响、伤口愈合时间以及复发等方面优于外剥内扎术。     

超声动脉健康评估技术测量正常颈动脉内-中膜厚度探讨

目的探讨测量颈动脉内-中膜厚度（CIMT）的方法,评估其临床应用价值。方法应用超声动脉健康评估软件自动测量133名健康者和62名动脉粥样硬化患者的颈总动脉、窦部和颈内动脉的内-中膜厚度,获得CIMT、血管年龄（VA）和冠心病（CHD）危险度,并进行统计学分析。结果健康组颈动脉不同部位的IMT差异有统计学意义（P〈0.05）。健康组CIMT和VA随着年龄增大而增大（P〈0.05）。动脉粥样硬化组的年龄、CIMT、VA和CHD危险度高于健康组（P〈0.05）。结论该技术能方便准确地测量CIMT,具有应用于临床评估动脉粥样硬化的潜力。     

丙氨酰谷氨酰胺二肽对大鼠急性胃溃疡的抑制作用

目的 在急性胃溃疡大鼠模型上评价丙氨酰谷氨酰胺二肽预防给药对不同因素所致大鼠急性胃溃疡的作用,探讨其可能作用机制。方法 建立水浸应激、无水乙醇和幽门结扎所致急性胃溃疡大鼠模型。每种胃溃疡模型均选用60只SD大鼠,随机分为模型组、西咪替丁（0.1 g/kg）组、麦滋林-S（1.0 g/kg）组以及丙氨酰谷氨酰胺二肽低（0.5 g/kg）、中（1.0 g/kg）及高（1.5 g/kg）剂量组。实验前分别给予不同药物灌胃,每天1次,连续3 d。以溃疡指数、胃液量、胃酸、游离酸、总酸及胃蛋白酶活性等为评价指标,观察不同剂量丙氨酰谷氨酰胺二肽灌胃给药对实验性胃溃疡的治疗效果。结果 在所选剂量范围内,丙氨酰谷氨酰胺二肽均可显著降低大鼠的溃疡指数（P<0.01）;高、中剂量丙氨酰谷氨酰胺二肽对溃疡的抑制作用与麦滋林-S基本相当。丙氨酰谷氨酰胺二肽还可显著抑制幽门结扎型大鼠胃液游离酸分泌（P<0.01）,降低胃蛋白酶活性（P<0.05）。结论 丙氨酰谷氨酰胺二肽对不同因素所致急性胃溃疡均具明确治疗效果;其抗胃溃疡作用机制除已知黏膜保护作用外,还可能与其对胃酸及胃蛋白酶抑制作用有关。     

内镜套扎术联合思他宁治疗食管静脉曲张破裂出血的疗效

目的：观察内镜套扎术（EVL）联合思他宁治疗食管静脉曲张破裂出血的疗效。方法：40例患者分为两组,实验组给予EVL＋思他宁治疗,对照组给予EVL＋氨甲苯酸治疗。观察两组患者的72h止血率、1个月再出血率、病死率。结果：实验组患者72h内止血率为95%,1个月内再出血率为10%,死亡率为5%;对照组患者72h止血率为80%,1个月内再出血率为15%,死亡率为10%。两组的72h止血率、1个月再出血率比较,差异均有统计学意义（P〈0.05）;两组的死亡率比较,差异无统计学意义（P〉0.05）。结论：EVL术联合思他宁治疗食管静脉曲张出血可有效提高止血率,降低再出血率。     

消肿祛痔汤治疗炎性痔临床观察

目的观察消肿祛痔汤治疗炎性痔的临床疗效。方法将120例炎性痔患者随机分成两组,每组各60例,治疗组采用消肿祛痔汤熏洗坐浴,对照组用1∶5000PP液熏洗坐浴,观察两组患者在用药前后症状、体征变化。结果治疗组治愈25例,有效33例,无效2例,总有效率为96.67%;对照组治愈16例,有效34例,无效10例,总有效率为83.33%,治疗组疗效明显优于对照组(P0.05)。治疗4d后,治疗组疼痛、水肿程度减轻,与对照组比较差异有统计学意义(P0.05);治疗7d后,治疗组疼痛、水肿程度显著减轻,与对照组比较差异有统计学意义(P0.01)。结论消肿祛痔汤治疗炎性痔有显著疗效。     

MLPA联合FISH在DMD基因突变产前诊断中的价值探讨

目的:分别采用多重连接探针扩增技术(MLPA)与荧光原位杂交技术(FISH)针对外周血及羊水标本分析杜氏/贝氏肌营养不良(DMD/BMD)患者DMD基因缺失/重复突变的类型。分析在DMD/BMD产前诊断中两者联合应用的诊断价值。方法:对2009年1月-2010年1月在我院行基因诊断的3个DMD/BMD家系共5位先证者及其15位女性亲属采用MLPA进行DMD基因的分析,获得缺失/重复片段范围,再通过BAC克隆制备相应区段的直标型FISH探针,并用X染色体着丝粒探针作为对照。分别采取MLPA和FISH技术对羊水标本进行检测,与分娩后取样的MLPA检测结果进行对照。结果:MLPA与FISH技术检测效果在外周血中完全一致,但3例羊水标本中采用MLPA进行检测有1例无法满足分析要求,1例出现假阳性结果,另1例为真阴性结果,MLPA联合用FISH检测结果均正确。结论:MLPA和FISH检测DMD基因缺失/重复在外周血中都能够获得准确的结果,但对于羊水标本MLPA联合FISH进行检测的准确性更高。     

内镜下套扎术联合药物治疗食管静脉曲张出血的近期疗效观察

目的探讨内镜下食管静脉曲张套扎术联合药物治疗肝硬化上消化道出血的近期疗效。方法回顾性分析2007年3月至2009年12月143例肝硬化患者行食管静脉曲张套扎术治疗的资料。结果食管静脉曲张套扎术术后联合普奈洛尔、抑酸剂、生长抑素等药物治疗,明显降低早期再出血、食管溃疡、门脉高压性胃病等发生率;肝功能C级患者再出血率明显高于A级及B级患者;肝硬化有脾切除加断流术史者与无脾切除加断流术患者比较,食管静脉曲张复发率低。结论食管静脉曲张套扎术术后联合药物治疗可降低食管静脉曲张复发、食管静脉曲张出血早期再发率,减少食管溃疡、门脉高压性胃病发生率。食管静脉曲张套扎术是治疗食管静脉曲张出血安全有效的方法,疗效确切,提高了患者生存率。     

Automatic and imperative motor activations in stimulus–response compatibility: magnetoencephalographic analysis of upper and lower limbs

The stimulus–response (S–R) compatibility effect refers to the difference in performance due to the spatial S–R relationship in choice reaction time. We investigated the mechanism of neural activities in S–R compatibility at the level of the primary motor cortices for upper and lower limbs responses using magnetoencephalography (MEG). In the S–R compatible task, subjects were required to respond on the same side of the stimulus light using either an upper or lower limb. In the incompatible task, subjects were required to respond in the reverse manner. Premotor times of upper and lower limbs were faster for the compatible response than for the incompatible response. The neuromagnetic brain activities related to response execution were estimated using a multi-dipole model. Stimulus-locked MEG indicated that the current moments of motor dipoles for both effectors occurred bilaterally and reached the first peak at a constant delay irrespective of whether the task was compatible or incompatible. This indicates that the neural activation of the primary motor cortex is automatically synchronized with the stimulus onset. Response-locked MEG showed that the peak current moment of the motor dipole contralateral to the response was stronger for the compatible task than for the incompatible one regardless of whether the responses were made using the upper or lower limbs. The MEG results suggest that automatic motor activation facilitates imperative motor activation for a compatible response, whereas it is not sufficient to prime imperative motor activation for an incompatible response.     

Forsythoside B protects against experimental sepsis by modulating inflammatory factors

The present study investigated the effects of Forsythoside B on an experimental model of sepsis induced by caecal ligation and puncture (CLP) in rats and elucidated the potential mechanism in cultured RAW 264.7 cells. Results showed that Forsythoside B concentration‐dependently down‐regulated the levels of TNF‐α, IL‐6 and high‐mobility group‐box 1 protein (HMGB1) in lipopolysaccharide (LPS)‐stimulated RAW264.7 cells, inhibited the IκB kinase (IKK) pathway and modulated nuclear factor (NF)‐ κB. Intravenous injection (i.v.) of Forsythoside B alone or plus Imipenem reduced serum levels of TNF‐α, IL‐6, HMGB1, triggering receptor expressed on myeloid cells (TREM‐1) and endotoxin, while the serum level of IL‐10 was up‐regulated and myeloperoxidase (MPO) in lung, liver and small intestine was reduced. Meanwhile, i.v. of Forsythoside B alone or plus Imipenem reduced CLP‐induced lethality in rats. These data indicated that the antisepsis effect of Forsythoside B is mediated by decreasing local and systemic levels of a wide spectrum of inflammatory mediators. Its antisepsis mechanism may be that Forsythoside B binds to LPS and reduces the biological activity of serum LPS, and inhibits NF‐κB activition. Our studies enhance the case for the use of Forsythoside B in sepsis. Forsythoside B itself has promise as a therapy for the treatment of sepsis in humans. Copyright © 2011 John Wiley & Sons, Ltd.