血液分析仪电阻抗法计数血小板临床应用评价

目的 通过与血小板测定的参考方法比较来评价电阻抗法用于血小板计数的临床价值.方法 检测标本按电阻抗法血小板计数＞100×109/L、(50～100)×109/L、(20～50)×109/L、＜20x109/L分为四组,分别用参考方法和血液分析仪测定同一标本血小板数,对测定结果进行统计学分析.结果 ＞100×109/L组和(50～100)×109/L组二种方法检测结果差异无统计学意义(P＞0.05),(20～50)×109/L、＜20×109/L组二种方法检测结果差异有统计学意义(P＜0.05).结论 电阻抗法对血小板数＜50×109/L标本血小板计数误差明显,可应用参考方法进行复检.     

Caco-2细胞模型验证指标的选择与评判

 目的建立Caco-2细胞模型并探讨验证该模型指标的选择与评判。方法在所建立的Caco-2单层细胞模型上,通过测定跨上皮细胞膜电阻(TEER),比较膜两侧碱性磷酸酶(ALP)活性,计算几种公认的标记物在单层模型转运参数等对Caco-2细胞单层可靠性进行评价。结果TEER在细胞单层形成过程中稳定增长,膜两侧培养液中ALP活性差异逐步加大,细胞旁及跨细胞转运标记物均呈现可预见的低或高表观渗透系数(P_app)值,P-gp标准底物非索非那定双侧转运流出率(ER)大于2。结论建立的Caco-2细胞模型在完整性、细胞极性、通透性以及P-gp表达等方面均符合胃肠吸收化合物机制研究的要求。     

规范式分段线性化法在非线性电阻网络故障诊断中的应用

本型文针对非线性电阻网络,提出了一种规范式分段线性化求所有解,并采用BPNN对非线性电阻网络硬故障进行诊断的方法,克服了用一般方法对非线性电阻网络故障诊断时易存在的漏诊问题。     

AC-970EO+血球计数仪的保养与堵孔处理

AC - 970EO +是瑞典生产,采用电阻抗法原理检测血细胞的全自动血球计数仪。在基层医院中,一般没有备用设备,仪器全天2 4h开机。在工作中总结经验,制定好仪器的保养方案,做好保养工作,正确分析和主动预防变换器上镶嵌的宝石小孔核心部件的堵塞,快速地排除仪器堵孔故障,确保仪器的正常运转。在保持工作畅顺、处理好医患关系及减少经济上的损失都有着积极的意义。本文介绍使用该仪器的保养工作与堵孔的处理。     

Ag／AgCl粉末电极和镀层电极在脑部EIT测量中的比较研究

目的：针对一种新型的医用Ag／AgCl粉末电极和传统的医用Ag／AgCl镀层电极，比较两者在脑部电阻抗断层成像（Electrical Impedance Tomography，EIT）测量中的性能差异。方法：基于自行开发的EIT系统，分别利用Ag／AgCl粉末电极和镀层电极，分别对物理模型和人体对象进行脑部电阻抗断层成像测量，并且通过提出的两个指标（高频信噪比和低频信噪比）及接触阻抗来评价电极系统的特性。结果：相对于Ag／AgCl镀层电极，Ag／AgCl粉末电极具有更好的抗噪性和稳定性。结论：Ag／AgCl粉末电极能更有效地满足脑部EIT监护的要求。     

心肌梗死与心包经双侧经络电阻抗失衡的相关研究

目的　探讨心肌梗死与心包经双侧经络电阻抗的相互关系 ,寻求无创伤性临床疾病的早期诊断。方法测量左右侧心包经上的内关穴与三阴交穴位之间的电流波形 ,计算其电阻抗值。结果　观察组显示两侧电阻抗差别有显著性意义 (P <0 0 1) ;对照组两侧差别无显著性意义 (P >0 0 5 )。结论　正常人双侧经络电阻抗的对称性、经络循行线的低阻性及双侧经络电阻抗失衡与其络属脏器病变有着密切的相关性 ,此项测定可能为心脏疾病提供一种新的早期诊断的方法。     

Glucocorticoids upregulate transepithelial electrical resistance and expression of tight junction-related protein in human trabecular meshwork cells

The trabecular meshwork is located at the anterior chamber angle, and is the main route for the outflow of aqueous humor. It is composed of perforated sheets of collagen and elastic tissue covered by trabecular meshwork (TM) cells, forming a filter with decreasing pore size as the canal of Schlemm is approached. TM cells have some endothelial properties, such as the presence of intercellular junctional complexes, particularly tight junctions (TJs). TJs form paracellular seals between adjacent cells and act as fences that segregate protein (and partially lipid) components of the apical and basolateral plasma membrane domains. Under the electron microscope, TJs appear as a series of discrete contacts between the lateral membranes of adjacent cells.     

Imaging Techniques of Electrical Impedance Tomography for Real-time Medical Monitoring

Electrical Impedance Tomography （EIT） is a new functional imaging technique. To monitor the development of the internal hemorrhage which can not be continuously detected by presented clinical imaging methods, the imaging techniques of EIT are proposed for the continuous monitoring. The individual image mapping technique and consecutive images displaying technique were both used. Internal bleeding within human body was simulated by the drinking of 0.3% saline water and was measured continuously by EIT over a long period of time. A sequence of EIT images were generated at a rate of one image per second. It was found that the variations of the amounts of the saline water drunk could be shown by the consecutive images of EIT. Therefore, the imaging techniques are useful to facilitate EIT to become a promising bedside monitoring tool for the patients suffering from the active ongoing bleeding.     

X线洗片机温控原理及温度失控原因探讨

本文叙述了医用X线洗片机的温度控制原理,对引起机器温度失控（高温或低温）的原因进行了系统的分析,并给出了相应的解决办法。