Enzyme-linked immunosorbent assay for Giardia-specific IgA in mouse intestinal secretions

We describe an ELISA for trophozoite-specific IgA in the intestinal secretions of mice infected with Giardia muris. Using this method, trophozoite-specific IgA was demonstrated in intestinal secretions of Giardia-infected immunocompetent BALB/c mice. Such antibody was undetectable in the intestinal secretions of Giardia-infected athymic (nude) mice. Immunocompetent BALB/c mice are able to clear G. muris infection whereas nude mice are not. The study provides evidence that the chronicity of G. muris infection in nude mice results from lack of intestinal trophozoite-specific IgA in these animals. By means of the ELISA, trophozoite-specific IgA was demonstrated in intestinal secretions from immunocompetent mice in the absence of protease inhibitors.     

用ELISA检测肺癌患者血清中Endostatin的变化

目的建立快速敏感的ELISA方法检测肺癌患者血清内皮抑素(Endostatin)。方法对41例肺癌患者及22例良性肺病患者进行血清Endostatin测定并与20例正常人进行比较。结果肺癌患者体内Endostatin水平高于良性肺病患者和正常人。结论快捷的ELISA检测法可以成为人们在临床及基础研究Endostatin的新方法。     

三种检测梅毒螺旋体血清学方法评估

目的 寻求一种敏感度和特异性高的方法用于输血前和临床诊断检查梅毒螺旋体抗原血清学试验。方法 应用TPPA、ELISA、TRUST等三种梅毒螺旋体血清学检测方法，对25例各期梅毒血清标本进行检测，对其敏感性及特异性进行比较。结果 25例梅毒血清标本，ELISA法和TPPA法检测阳性率为100％，TRUST法为93％；经稀释法检测，ELISA法较TPPA法敏感。对815例临床标本检测，ELISA方法与TPPA法的检出符合率为100％。结论 ELISA法操作简单，结果判断可标准化，特异性强，敏感性高；既可用于过筛试验，又可用于筛检阳性样本的确认。     

脑囊虫病患者血清和脑脊液IL-6和SIL-2R的水平及其与癫痫发作的关系

目的测定脑囊虫病患者血清和脑脊液IL-6和SIL-2R水平,并探讨其与癫痫发作的关系。方法应用双抗体夹心ELISA法测定45例脑囊虫病患者和43例对照组患者血清和脑脊液IL-6利SIL-2R的水平,其中脑囊虫病组又分为癫痫组(23例)和非癫痫组(22例),分别进行了比较分析。结果脑囊虫病组血清SIL-2R水平明显高于对照组(P<0.05);而IL-6水平与对照组比较无显著性差异(P>0.05)。脑囊虫病组脑脊液SIL-2R和IL-6水平均较对照组低(P<0.01,P<0.05);脑囊虫病组血清SIL-2R和IL-6水平与脑脊液SIL-2R和IL-6水平均呈正相关(r=0.322,P<0.05;r=0.768,P<0.01)。脑囊虫病癫痫组血清SIL-2R和IL-6水平与非癫痫组比较均无显著性差异(P>0.05);而脑脊液SIL-2R和IL-6水平癫痫组明显高于非癫痫组(P<0.01)。结论脑囊虫病患者外周血和中枢神经系统存在免疫激活,且SIL-2R和IL-6可能参与了癫痫发生过程。     

Enzyme-linked Immunosorbent Assay of Pro-gastrin-releasing Peptide for Small Cell Lung Cancer Patients in Comparison with Neuron-specific Enolase Measurement

Our previous study demonstrated that pro-gastrin-releasing peptide(31–98), or ProGRP, is a specific tumor marker in patients with small cell lung carcinoma (SCLC). Using a newly developed, highly sensitive enzyme-linked immunosorbent assay (ELISA) for ProGRP, we analyzed 1,446 samples including those obtained from 478 lung cancer patients to evaluate the clinical usefulness of this ELISA. Several properties indicated that ProGRP is a useful tumor marker for SCLC. First, ProGRP was specifically elevated in SCLC patients. In non-SCLC patients and patients with non-tumorous lung diseases, its serum level was very rarely elevated. Secondly, ProGRP was a reliable marker, in terms of the marked elevation of serum ProGRP levels in SCLC patients. Thirdly, serum ProGRP levels were elevated in SCLC patients even at a relatively early stage of this disease. Fourthly, changes in the serum ProGRP level showed an excellent correlation with the therapeutic responses in SCLC patients. Neuron-specific enolase (NSE) is accepted as a tumor marker of SCLC patients. With the aim of comparing ProGRP and NSE as tumor markers for SCLC patients, we measured serum NSE levels in all samples collected in the present study. We found that ProGRP was superior to NSE in terms of sensitivity, specificity and reliability. Therefore, we consider that ProGRP can play a major role as a clinical tumor marker for SCLC patients.     

幽门螺旋菌菌体蛋白质初步研究

用聚丙烯酰胺凝胶电泳(SDS—PAGE)分析幽门螺旋菌(HP),其超声粉碎和冻融抗原有7条主要蛋白质条带,分子量为71kd、62kd、58kd、51kd、29kd、22kd和12kd。免疫印迹法发现HP100kd免疫性强、特异性高。HP外膜蛋白质分子量主要为71kd、58kd、29kd和12kd,HP不同林之间SDS—PAGE图谱相似,但与空肠弯曲菌和结肠弯曲菌明显不同。超声粉碎和冻融处理HP后主要蛋白质条带相同,冻融抗原制备简单,适宜作为ELISA使用抗原。     

ELISA法检测中临界值标本的制备及临床应用

在应用酶联免疫吸附试验(ELISA)的检测过程中,往往临界值标本的作用比阳性标本的意义更大,它不仅是室内质控的重要材料,而且还可以用来监测试剂质量的变化情况。一般试剂生产厂家在试剂盒中只提供了强阳性标本,而未能提供临界值标本。为此,我们在实际工作中作了一些探索,已成功地制备出乙肝指标的临界值标本,并在实际工作中得以应用,取得了一定的成效。观予以报道。1材料与方法1.1材料:乙肝“两对半”ELISA法检测试剂盒(上海荣盛试剂厂生产)。阴性稀释血清标本:除相应所要求的乙肝“两对半”指标均为阴性外,其余各项生化指标均要求正常,…     

病毒性脑炎患者S-100b蛋白浓度与临床的相关性

目的 观察病毒性脑炎急性和恢复期患者血液和脑脊液的S-100b蛋白浓度变化，探讨其与异常脑电图和影像学低密度灶体积等方面的相关性。方法 42例病毒性脑炎住院患者为观察组，25例与观察组相匹配的阑尾或胆囊摘除手术病人以及健康体检者为对照组，采用双抗体夹心ELISA法，检测其血液及脑脊液100b蛋白浓度；并应用SPSS10．0统计软件包进行统计学分析。结果病毒性脑炎急性期患者血液和脑脊液S-100b蛋白显著高于恢复期患者和对照组（P〈0．01）；血液和脑脊液S-100b蛋白浓度与低密度灶体积均呈显著正相关（rCSF=0．756，P〈0．01；r血液=0．685，P〈0．01）；轻度、中度及重度异常脑电图患者脑脊液S-100b蛋白浓度均高于血液（P〈0．01）；重度、中度异常脑电图患者血液及脑脊液S-100b蛋白浓度均高于轻度（P〈0．01）。结论脑脊液或血液中的S-100b蛋白浓度反映了神经胶质细胞的损害程度，也是脑组织破坏后较合适的生化标记物，对监测病毒性脑炎患者的病情变化和疗效观察具有重要价值。