糖尿病患者血清肿瘤坏死因子C反应蛋白水平改变及其与牙周病的关系

目的检测2型糖尿病合并牙周病患者血中炎性标志物C反应蛋白(CRP)和肿瘤坏死因子(TNF)-α水平的变化,探讨二者与糖尿病牙周病的关系。方法对584例2型糖尿病患者进行牙周病的筛查,检测血CRP、TNF-α与健康体检配对人群进行相关指标比较。结果2型糖尿病牙周病的患病率高达63.7%,对照组为21.6%。糖尿病合并牙周病患者CRP为(8.4±0.9)mg/L,糖尿病无牙周病患者为(5.9±0.8)mg/L,两者差异有统计学意义(P<0.01)。TNF-α在糖尿病合并牙周病组为(234±28)ng/L,明显较糖尿病无牙周病组的[(178±28)ng/L]增高(P<0.01)。结论糖尿病与牙周病两者互相影响,相互促进,导致牙周病加重及糖尿病大血管并发症进展。     

清肠泡腾栓调控溃疡性结肠炎细胞因子的实验研究

目的探讨中药肠道泡腾栓剂治疗溃疡性结肠炎的免疫学疗效机制。方法实验分设正常对照、模型对照、空白泡腾栓、清肠泡腾栓、清肠栓、柳氮磺胺吡啶栓共6个组,ELISA法检测血清、结肠上清TNF-α仪、IL-13；免疫组化、原位杂交检测IL-8(mRNA)阳性表达。结果模型对照组血清TNF-α较正常对照组升高(P＜0．05),结肠黏膜IL-8(mRNA)阳性表达明显增强(P＜0．01)；清肠泡腾栓明显降低结肠上清TNF-α含量以及IL-8(mRNA)阳性表达,与模型组比较差异有显著性(P＜0．05,P＜0．01)；清肠泡腾栓对IL-13的调控作用不明显,与模型对照组比较差异无显著性(P＞0．05)。结论清肠泡腾栓通过抑制促炎细胞因子而对溃疡性结肠炎产生治疗作用。     

蕲蛇酶对大鼠脑缺血再灌注损伤的保护作用

目的进一步探讨蕲蛇酶对大鼠脑缺血再灌注损伤的保护作用及其机制。方法采用线栓法制备大鼠大脑中动脉闭塞后再灌注(MCAOR)模型,观察大鼠MCAOR时神经功能状态,同时,TTC染色测脑梗死面积,电镜观察脑血管超微结构,放免法测定血浆血栓素B2及6-酮前列腺素F1α含量。用药组分别在缺血即刻或再灌注即刻静脉给药,观察比较模型组和蕲蛇酶所有给药组之间上述指标的变化。结果蕲蛇酶所有给药组都能有效改善MCAOR大鼠神经功能缺失症状,明显减小梗死灶,调节血栓素B2/6-酮前列腺素F1α,保护血管内皮细胞,维持微血管正常结构。结论蕲蛇酶对脑缺血再灌注损伤保护作用可能与维持血栓素B2/6-酮前列腺素F1α平衡、改善脑微循环有关。     

银杏内酯B对慢性炎症血管生成的抑制作用

目的研究银杏内酯B对慢性炎症血管生成的作用及部分作用机制。方法比色法测定小鼠慢性肉芽肿气囊模型血管生成指数，组织形态学方法检测气囊病理变化；放射免疫方法测定白介素-1β(IL-1β)含量；L929生物测定法测定肿瘤坏死因子(TNF-α)含量；RT-PCR法检测IL-1β和TNF-α mRNA的表达。结果银杏内酯B可显著抑制模型小鼠的血管指数，与病理观察结果相符；银杏内酯B可显著抑制模型小鼠血清中IL-1和TNF-α的分泌；能显著抑制PMA诱导的U937细胞IL-1β和TNF-α的分泌及其mRNA的表达。结论银杏内酯B能抑制小鼠慢性炎症性血管生成模型的血管生成，能抑制促血管生成细胞因子IL-1β和TNF-α的转录及表达，这可能是其抑制慢性炎症血管生成的机制之一。     

细脚拟青霉总多糖对人外周血单核细胞TNF-α水平及增殖活性的影响

目的提取分离细脚拟青霉总多糖 (PtPs) ,探讨其对人外周血单核细胞 (PBMC)的调节作用。方法采用水提醇沉淀法提取PtPs成分 ;用不同浓度的PtPs单独或协同脂多糖 (LPS)在体外刺激PBMC ,酶联免疫法测定TNF α的水平 ,溴化四唑蓝法测定增殖活性。结果所得PtPs制品的纯度为 76 .1% ;适当剂量的PtPs (10 0～ 5 0 0 μg/ml)能够单独或协同LPS提高PBMC对TNF α的分泌 ;还可剂量依赖性地促进PBMC的增殖活性。结论PtPs对体外培养的PBMC具有激活作用 ,可能是发挥免疫药理作用的主要活性成分。     

1α,25-二羟维生素D_3对患者PBMC分泌的IFN-α和IL-10水平的影响

目 的 探 讨 １α，２５－二羟 维生 素 Ｄ_３（１α，２５－（ＯＨ ）２Ｄ ）对 系 统性 红斑 狼 疮（ＳＬＥ）患 者 外周 血 单个 核 ３细 胞（ＰＢＭ Ｃ）分 泌的 白细 胞 介素 （ＩＬ）－１０ 和 干扰 素 （ＩＦＮ ）－α水平 的 影响 。方法 ２０ 例 ＳＬＥ 患 者和 １０ 名健 康 志愿者 ＰＢＭ Ｃ 的提 取 采 用 Ｆｉｃｏｌ密 度 梯 度 离 心 法 ，ＳＬＥ 组 和 对 照 组 在 不 加 或 加 入 １α，２５－（Ｏ Ｈ ）２Ｄ３ 的 情 况 下 孵 育 ７２ ｈ收 集培 养 上清 ，上 清液 ＩＬ－１０ 和 ＩＦＮ－α水平 检 测采 用酶 联 免疫 吸附 试 验（ＥＬＩＳＡ ）。结 果 与 正常 对 照组 相比 ，ＳＬＥ组 ＰＢＭ Ｃ 中 ＩＬ－１０ 和 ＩＦＮ－α水 平 明 显 增高 （Ｐ＜０．０１）。０．０１ ｍ ｏｌ／Ｌ １α，２５－（Ｏ Ｈ ）２Ｄ３ 的 加 入 明 显 抑 制 了 ＳＬＥ 组ＰＢＭ Ｃ 中 ＩＦＮ －α的 产 生 （Ｐ＜０．０１），增 加 了 ＳＬＥ 组 ＰＢＭ Ｃ 中 ＩＬ－１０ 的 产 生 （Ｐ＜０．０１），但 对 正 常 对 照 组 ＩＦＮ －α和ＩＬ－１０ 水平 无明 显 影响 。结 论 １α，２５－（ＯＨ ）２Ｄ 可 能 抑制 体外 培 养的 ＳＬＥ 患 者 ＰＢＭ Ｃ ＩＦＮ －α的产 生     

Differential effects of cadmium administration on peripheral blood granulocytes in rats

Infiltration of circulatory inflammatory cells is a common histopathological finding in target organs following cadmium administration, but there is paucity of data concerning their activity. In this study, the effects of sublethal (1 mg/kg) cadmium on peripheral blood polymorphonuclear (PMN) cells were examined 48 h following administration in rats, when tissue (liver and lung) infiltration of these cells was observed. Cadmium administration resulted in systemic inflammatory cytokine and acute phase response with an increase in circulatory neutrophil numbers and cells that express CD11b molecules. Rise in basic aspects of oxidative activity including intracellular myeloperoxidase (MPO), reactive oxygen (nitroblue tetrazolium/NBT cytochemical assay) and nitrogen (Griess assay) species production was observed in PMNs from cadmium-administered rats. A decrease in levels of mRNA for IL-1β, TNF-α and IL-6 was noted, but production of these cytokines was affected differentially. Described effects of cadmium on PMNs add further to the understanding of inflammatory potential of this environmental contaminant.     

The Biological Actions of Dehydroepiandrosterone Involves Multiple Receptors

Dehydroepiandrosterone has been thought to have physiological functions other than as an androgen precursor. The previous studies performed have demonstrated a number of biological effects in rodents, such as amelioration of disease in diabetic, chemical carcinogenesis, and obesity models. To date, activation of the peroxisome proliferators activated receptor alpha, pregnane X receptor, and estrogen receptor by DHEA and its metabolites have been demonstrated. Several membrane-associated receptors have also been elucidated leading to additional mechanisms by which DHEA may exert its biological effects. This review will provide an overview of the receptor multiplicity involved in the biological activity of this sterol.     

HIF1α-siRNA and gemcitabine combination-based GE-11 peptide antibody-targeted nanomedicine for enhanced therapeutic efficacy in pancreatic cancers

Abstract

Pancreatic cancer is one of the deadliest cancers across the world with an average 5-year survival rate of less than <6%. In this study, gemcitabine (GEM) and HIF1α-siRNA loaded GE-11 peptide conjugated liposome was successfully prepared and evaluated for its antitumor efficacy in pancreatic cancer cells. The GE11 increased the targeting specificity of liposome carrier and increased the intracellular concentrations in the cancer cells. Furthermore, synergistic combination of GEM and HIF1a-siRNA exhibited remarkable improvement in the declining of cancer cell proliferations. siRNA could effectively decrease the expression of HIF1a gene in the cancer cells. Importantly, GE-11 peptide-conjugated GEM/siRNA-loaded liposomes (GE-GML/siRNA) increased the total amount of apoptosis cells with higher proportion of cells in late apoptosis phase. GE-GML induced remarkable apoptosis of cancer cells and induced chromatin condensation and nuclear fragmentation which are considered to be typical features of apoptosis and cell death. GE-GML/siRNA showed a significant reduction in the tumour burden suggesting the superior anticancer efficacy of this formulation. GE-GML/siRNA showed four-fold reduction in tumour compared to control and two-fold reduction compared to GE-GML, respectively. Overall, present work lays foundation for the combination of GEM and HIF1a-siRNA loaded in a targeted nanocarrier system as a unique therapeutic option in pancreatic cancer treatment.