IL-20在寻常型银屑病患者皮损和PBMC中表达的研究

为了观察IL 2 0在寻常型银屑病患者皮损和外周血单个核细胞 (PBMC )中的表达情况 ,用免疫组化、免疫荧光和RT PCR方法检测IL 2 0在寻常型银屑病患者皮损处和正常人相应部位皮肤及PBMC中的表达。结果发现 ,用上述方法在正常人和银屑病患者皮肤中均不能检测到IL 2 0的表达。RT PCR检测发现 ,IL 2 0在寻常型银屑病患者PBMC中的阳性率为 2 5 % ,在正常人PBMC中的阳性率为 87 5 %。由此得出结论 ,虽然皮肤是IL 2 0作用的靶器官 ,但其本身并不表达IL 2 0。PBMC表达IL 2 0 ,但寻常型银屑病患者PBMC中IL 2 0的表达显著低于正常人 (P <0 0 1)。     

Projections from Areas 18 and 19 to Cat Striate Cortex: Divergence and Laminar Specificity

The results of electrical stimulation experiments [Bullier et al., (1988) Exp. Brain Res., 70, 90 - 98] demonstrated that afferents from areas 18 and 19 contact different functional types of neurons in area 17. We were therefore interested in examining whether these results could be explained by differences in the morphology of the terminals of these two groups of afferent connections to area 17. We also wanted to confirm, by a direct method, our earlier results [Salin et al. (1989) J. Comp. Neurol., 283, 486 - 512] that cortical afferents to area 17 in the cat present extensive divergences. We therefore placed small injections of anterograde tracers in areas 18 and 19 and examined the laminar distributions of terminals thus revealed and the extent of the surface of area 17 contacted by these terminals. Three tracers were used: wheat germ agglutinin - horseradish peroxidase (WGA - HRP), Phaseolus vulgaris leucoagglutinin (Pha-L) and biocytin. The results show that the divergence of these afferent connections are very extensive: 7 - 8 mm in the rotrocaudal direction and 3.5 - 6 mm in the mediolateral direction. In other words, neurons located in a region a few hundreds micron wide in areas 18 or 19 contact a region of area 17 covering several millimeters. Corticocortical connections are therefore not organized in a point-to-point fashion but are strongly divergent. The laminar distributions of terminals from areas 18 and 19 displayed a specific pattern. Area 19 projects most heavily to layers 5 and 6, also terminates in layers 1 - 3 and very little is present in layer 4. In contrast, the afferent terminals from area 18 are heaviest in layers 1, 2, 3, 4A and 5 and are rare in layer 6. Injections placed at different depths in area 18 revealed that upper layer neurons in that area mostly project to layers 1, 2, 3 and 5 in area 17, whereas lower layer neurons send their heaviest projections to layers 4A, 5 and 6 and hardly project to layers 1, 2 and 3.     

Prefrontal cortical projections to the cholinergic neurons in the basal forebrain

The prefrontal cortex (PFC) projections to the basal forebrain cholinergic cell groups in the medial septum (MS), vertical and horizontal limbs of the diagonal band of Broca (VDB and HDB), and the magnocellular basal nucleus (MBN) in the rat were investigated by anterograde transport of Phaseolus vulgaris leuco-agglutinin (PHA-L) combined with acetylcholinesterase (AChE) histochemistry or choline acetyltransferase (ChAT) immunocytochemistry. The experiments revealed rich PHA-L-labeled projections to discrete parts of the basal forebrain cholinergic system (BFChS) essentially originating from all prefrontal areas investigated. The PFC afferents to the BFChS display a topographic organization, such that medial prefrontal areas project to the MS, VDB, and the medial part of the HDB, whereas the orbital and agranular insular areas predominantly innervate the HDB and MBN, respectively. Since the recurrent BFChS projection to the prefrontal cortex is arranged according to a similar topography, the relationship between the BFChS and the prefrontal cortex is characterized by reciprocal connections. Furthermore, tracer injections in the PFC resulted in anterograde labeling of numerous "en passant" and terminal boutons apposing perikarya and proximal dendrites of neurons in the basal forebrain, which were stained for the cholinergic marker enzymes. These results indicate that prefrontal cortical afferents make direct synaptic contacts upon the cholinergic neurons in the basal forebrain, although further analysis at the electron microscopic level will be needed to provide conclusive evidence.     

中国芦荟治疗寻常痤疮临床研究

目的：观察中国芦荟治疗寻常痊疮的疗效。方法：全叶组41例外用芦荟全叶，叶肉组40例外用芦荟叶肉，对照组30例外用达芙文凝胶治疗。结果：3组总有效率分别为73．2％，70．0％，76．7％，经统计学处理无显著性差异(P＞0．05)。结论：中国芦荟全叶和叶肉外用治疗寻常痊疮疗效较好，但叶肉更安全，患者依从性好。     

夏枯草注射液为主治疗中晚期胃、大肠癌30例临床观察

选符合入选条件的中晚期胃、大肠癌住院患者50例，随机分为治疗组30例、对照组20例。治疗组予夏枯草注射液静滴，对照组口服平消胶囊，疗程均为30天。结果：治疗组中医症侯综合疗效明显优于对照组(u＝2．5200，P＝0．0122)；生存质量明显提高，且优于对照组(t＝2．4776，P＝0．0174)；治疗组每治疗10人，可较对照组减少1例实体瘤进展(PD)患者(NNT＝10);治疗组IFN—γ明显升高(t＝2．3960，P＝0．0204)，IL12、IFN—γ升高优于对照组(t＝2．9720，P＝0．0047；t＝2．1944，P＝0．0361)。结论：夏枯草注射液对中晚期胃与大肠癌证属脾胃或大肠湿热证者有较好的应用价值，作用优于平消胶囊。     

中药愈银方治疗寻常型银屑病的临床研究

目的:观察中药复方愈银方治疗寻常型银屑病的临床疗效及对红细胞、血小板指数的影响。方法:采用随机法将病人分为两组,治疗组44例,口服愈银方,日1剂,分2次水煎服;对照组25例,口服复方青黛丸,每次6g,每日3次。疗程8周。并检测治疗前后红细胞及血小板指数。结果:治疗组总有效率为93.18％,对照组总有效率为72.00％,两者相比有显著性差异(P<0.05)。且治疗前后的红细胞、血小板指数得到改善。结论:愈银方是治疗寻常型银屑病的有效方剂,值得进一步深入研究。其疗效机理可能与改善红细胞及血小板指数有关。     

氦-氖激光耳穴照射加针灸治疗寻常型痤疮36例

观察氦 氖激光耳穴照射加针灸治疗寻常型痤疮的临床疗效 ,6 8例寻常型痤疮患者随机分为观察组 (36例 ,以氦 氖激光耳穴照射加针灸治疗 )和对照组 (32例 ,单纯针灸治疗 )。结果 :观察组治愈率为 77 8% ,对照组为 4 6 9% ,P <0 0 5。提示氦 氖激光耳穴照射加针灸治疗寻常型痤疮疗效确切。     

柳穿鱼的生药研究

通过时柳穿鱼药材性状、显微特征及理化鉴别的分析研究，找到了鉴别特征，为该药材的使用和进一步研究提供了鉴定依据。     

羧甲基壳聚糖银、锌、铈的合成及抑菌实验的研究

报道了羧甲基壳聚糖银、锌、铈的合成及其混合物对金黄色葡萄球菌、铜绿假单孢菌、大肠埃希氏菌、肺炎克雷伯氏菌和变形杆菌的抑菌作用 ,并与磺胺嘧啶银进行比较 ,结果发现 ,羧甲基壳聚糖银、锌、铈水溶液 (含量为 0 .6 95mg/ml,Ag、Zn、Ce含量分别为 0 .2 5 2× 10 6、5 .2 8× 10 6、2 .15 4× 10 6)对上述 5种细菌 (含量为10 4CFU/ml)杀菌率为 96 %以上 ,与 1.0mg/ml磺胺嘧啶银作用相当