Effect of anterior overbite malocclusion on smile esthetics in adult patients

ObjectiveTo investigate whether overbite affects smile esthetics.MethodsThis study involved 106 patients with complete pretreatment records. Lateral cephalometric tracings were used to measure hard tissue variables. Frontal smile and upper occlusal photographs were used to measure nine smile esthetic variables: arc ratio, number of teeth, upper incisor exposure, upper midline, buccal corridor ratio, smile index, archform index, lower teeth exposure, and interlabial gap. The patients were classified into three groups according to their overbites (B1: 0–4 mm, B2: >4 mm, and B3: <0 mm). Analysis of variance was performed to compare 14 cephalometric measurements and the 9 smile esthetic variables. Multiple linear regression analysis was performed to determine the influencing cephalometric factors.ResultsOnly upper incisor exposure was significantly different among the groups. In the multiple linear regression analysis, upper incisor exposure was positively associated with the distance from the upper incisor to the palatal plane in Group B2. No significant correlations were observed between cephalometric measurements and smile variables in Groups B1 and B3.ConclusionsSmile variables were not significantly different among patients with various overbite malocclusions with the exception of upper incisor exposure. Overbite malocclusions do not appear to influence smile esthetics in adult patients.     

项目教学法在高职高专《美容解剖学》教学中的应用研究

目的:探讨项目教学法在高职高专《美容解剖学》中的应用价值。方法:将我校2013级专科医疗美容技术专业学生50人(男5人,女45人),随机分为实验组和对照组进行《美容解剖学》的教学,实验组采取项目教学法,对照组采取传统教学法。结果:两组期末总评成绩比较,实验组平均成绩为(91.2±6.7)分,对照组的平均成绩为(83.5±7.1)分。实验组成绩明显高于对照组,差异有统计学意义(P0.05)。授课满意度调查结果显示,实验组学生的满意度高于对照组。结论:项目教学法在高职高专《美容解剖学》中的应用有明显效果。     

隐美探微--性人文的隐性文化

性人文旨意是和性有关的文化生活及异性之间对异文化产生的实质意义。早在上古时代，在人们群落当中已经对性文化艺术有了启动。经过漫长的发展普及、曾遭受过来自社会不同方面的磨难。为避免重重阻力和非议，采取隐性方式交流于民间，同时也必然会影响到文学艺术创作。于此，本章用“隐美探微”来命题，提示隐性美存在于我们的生活艺术之中。当人们真正理解了性人文存在隐性美的意义时，才会使当代人对性意识形态有所触动。     

Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element

Background: Transposon‐mediated transgenesis is an excellent method for creating stable transgenic lines and insertional mutants. In the chordate Ciona intestinalis, Minos is the only transposon that has been used as the tool for germline transformation. Adding another transposon system in this organism enables us to conduct genetic techniques which can only be realized with the use of two transposons. Results: In the present study, we found that another Tc1/mariner superfamily transposon, sleeping beauty (SB), retains sufficient activity for germline transformation of C. intestinalis. SB shows efficiencies of germline transformation, insertion into gene coding regions, and enhancer detection comparable to those of Minos. We have developed a system for the remobilization of SB copies in the C. intestinalis genome by using transgenic lines expressing SB transposase in the germ cells. With this system, we examined the manner of SB mobilization in the C. intestinalis genome. SB shows intrachromosomal transposition more frequently than Minos. Conclusions: SB‐based germline transformation and the establishment of a new method that uses its frequent intrachromosomal transposition will result in breakthroughs in genetic approaches that use C. intestinalis together with Minos. Developmental Dynamics 242:30–43, 2013. © 2012 Wiley Periodicals, Inc.     

Sleeping Beauty转座子介导敲低PEX2转基因载体的构建

【摘要】 目的 借助Sleeping Beauty（SB）转座系统在小鼠肝脏中稳定过表达原癌基因MYC诱导小鼠肝癌形成，并在SB转座子pT3EF1α c Myc基础上构建可在小鼠体内敲低PEX2的重组转基因载体。方法 借助水动力法尾静脉注射（Hydrodynamic tail vein injection, HTVI）和SB转座子系统向小鼠肝脏导入携带目的基因MYC的转座子质粒，诱导小鼠肝癌形成。 以载体为模板，分别扩增U6 shNC、U6 shPEX2序列，通过T4连接获得目标载体pT3EF1α c Myc shNC、pT3EF1α c Myc shPEX2。结果 ①实验组小鼠成功诱导出肝癌。②经酶切和测序等方法鉴定，构建质粒完全正确。③细胞瞬时转染实验质粒pT3EF1α c Myc shPEX2及其对照质粒pT3EF1α c Myc shNC，确定在小鼠肝癌细胞Hepa1 6中实验组质粒可以降低PEX2的mRNA水平。结论 借助 HTVI技术和SB转座系统过表达c Myc成功诱导小鼠肝癌形成，并在SB转座子质粒pT3EF1a c Myc基础上构建了可在小鼠肝癌细胞Hepa1 6中敲低PEX2基因的重组转基因载体。     

行动学习在医疗美容技术专业建设中的运用

医疗美容技术专业面临课程设置盲目、师资缺乏、实训室设备简陋、校企合作不佳等主要问题,导致学生在实际从业过程中缺乏甚至不具备美容行业各岗位所必需的职业能力。行动学习是国际上较为成熟的管理培训方法之一。其强调"从做中学"、"从反思中学"以及"在学习中学会学习"的有机结合。将行动学习引入到医疗美容技术专业建设中,按照行动学习的理念进行专业建设,可实现该专业教师与学生职业能力的互动提升。     

2 241例中国城市女性中医体质状况调查分析

目的考察中国城市女性的体质状况,为个体化养生养美服务。方法在北京、上海、广州等9个省会城市和直辖市召集女性志愿者2 241名,以网络问卷的方式对受访者进行中医体质类型调查,按照中华中医药学会颁布的《中医体质分类与判定》判定中医体质类型。按年龄段、职业、婚姻状况和地域进行分层,考察9种体质类型的频数分布。结果中国城市女性平和质占21.95%,在不同年龄段、不同职业、不同婚姻状况和不同地域的女性偏颇体质频数分布中,气虚质、阳虚质、阴虚质频数居前3位。结论应针对气虚质、阳虚质、阴虚质对中国城市女性开展养生养美等保健服务。