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51.
We characterized 168 junctional regions of T-cell receptor delta (TCRD) rearrangements from 116 children with acute lymphoblastic leukemia (ALL) (101 with precursor B-cell ALL, 15 with T-cell ALL). Application of 101 allele-specific oligonucleotide (ASO) probes representing 85 Vdelta2Ddelta3, 10 Ddelta2Ddelta3, 3 Vdelta1Jdelta1, 1 Vdelta3Jdelta1, and 2 Ddelta2Jdelta1 junctions for the detection of minimal residual disease (MRD) revealed detection levels of 10(-4) to 10(-6) leukemia cells in the vast majority of cases (93 of 101). Of interest was that neither the N, D, P (nontemplated, diversity, palindromic) content and length of the junctional regions nor the number of nucleotides deleted from the flanking V, D, or J (variable, diversity, joining) elements correlated with the sensitivity of ASO probes. These data indicated that in ALL TCRD rearrangements can serve as suitable tools for the detection of MRD irrespective of the specific composition of the junctional region. 相似文献
52.
Camille Daste François Rannou Luc Mouthon Katherine Sanchez Alexandra Roren Vincent Tiffreau Éric Hachulla Philippe Thoumie Jean Cabane Emmanuel Chatelus Jean Sibilia Serge Poiraudeau Christelle Nguyen 《Seminars in arthritis and rheumatism》2019,48(4):694-700
Background
To estimate patient acceptable symptom state (PASS) and minimal clinically important difference (MCID) for patient-reported outcomes in systemic sclerosis (SSc).Methods
We conducted a secondary analysis of the SCLEREDUC trial, a 12-month randomized controlled trial comparing the efficacy of physical therapy to usual care in 220 SSc patients followed-up from September 2005 to October 2010. Self-rated state and change in patient health at 12 months were assessed by using 2 external anchors extracted from the Medical Outcomes Study 36-Item Short-Form. Patients who self-rated their health as “excellent”, “very good” or “good” were the PASS group and those who self-rated their health change as “somewhat better” were the MCID group. Main outcomes were the estimates of PASS by using the 75th percentile method and of MCID by using the mean change in scores method for pain and activity limitation.Results
PASS (95% confidence interval) and mean (SD) MCID estimates at 12 months were 53.75 (34.00 to 68.00) and ?6.74 (32.02) for the joint-pain visual analog scale (range 0–100), 1.41 (1.13 to 1.63) and ?0.21 (0.48) for the Health Assessment Questionnaire (HAQ, range 0–3), 1.27 (1.07 to 1.62) and ?0.13 (0.45) for the scleroderma HAQ (range 0–3), 26.00 (17.00 to 37.00) and -3.38 (9.87) for the Cochin Hand Function Scale (range 0–90), and 19.40 (17.20 to 21.90) and ?5.69 (6.79) for the McMaster-Toronto Arthritis Patient Preference Disability Questionnaire (range 0–30), respectively.Conclusions
We provide, for the first time, the PASS and MCID estimates for pain and activity limitation in SSc.53.
54.
目的:系统性评价利奈唑胺对2013~2014年耐甲氧西林金黄色葡萄球菌(MRSA)临床分离株细菌生物膜(BBF)的活性及体内外抗菌效果。方法:体外试验测定最低抑菌浓度(MIC);最低杀菌浓度(MBC);最小抑制BBF浓度(MBIC)和最低BBF消除浓度(MBEC);活菌计数法绘制时间-杀菌曲线(KCs);体内试验采用小鼠MRSA全身感染模型,尾静脉给药保护小鼠后测定半数有效剂量(ED50);建立免疫低下小鼠MRSA大腿感染模型,记录尾静脉给药24 h后大腿组织菌量的变化。结果:利奈唑胺对2013~2014年临床分离的60株MRSA均敏感;对金黄色葡萄球菌BBF的MBIC值与万古霉素相当,敏感性显著高于阿莫西林;体内试验中,利奈唑胺对全身感染小鼠有很好的治疗效果,ED50小于万古霉素与阿莫西林;对免疫低下MRSA大腿感染模型小鼠的保护作用也要优于万古霉素和阿莫西林。结论:利奈唑胺对2013~2014年分离的MRSA临床菌株体内外活性均较高,尤其对MRSA的细菌生物膜也显示了极强的抑制作用。 相似文献
55.
Segundo Moran Marlene L pez-S nchez Mar a del Pilar Milke-Garc a Gustavo Rodr guez-Leal 《World journal of gastroenterology : WJG》2021,27(22):3050-3063
Minimal hepatic encephalopathy (MHE) corresponds to the earliest stage of hepatic encephalopathy (HE). MHE does not present clinically detectable neurological-psychiatric abnormalities but is characterized by imperceptible neurocognitive alterations detected during routine clinical examination via neuropsychological or psychometrical tests. MHE may affect daily activities and reduce job performance and quality of life. MHE can increase the risk of accidents and may develop into overt encephalopathy, worsening the prognosis of patients with liver cirrhosis. Despite a lack of consensus on the therapeutic indication, interest in finding novel strategies for prevention or reversion has led to numerous clinical trials; their results are the main objective of this review. Many studies address the treatment of MHE, which is mainly based on the strategies and previous management of overt HE. Current alternatives for the management of MHE include measures to maintain nutritional status while avoiding sarcopenia, and manipulation of intestinal microbiota with non-absorbable disaccharides such as lactulose, antibiotics such as rifaximin, and administration of different probiotics. This review analyzes the results of clinical studies that evaluated the effects of different treatments for MHE. 相似文献
56.
应用聚合酶链反应(PCR)扩增IgH重排产生的CDR-Ⅱ序列,检测23例急慢性B-淋巴细胞白血病患者。20例发生IgH重排,其中4例出现两种基因扩增产物。6例完全缓解的患者,3例检出异常重排的IgH基因,并先后复发。本文应用Southern blot法和PCR进行了对照性研究。 相似文献
57.
《Gait & posture》2021
BackgroundIndividuals with lower limb amputation are routinely assessed with a variety outcome measures, however there is a lack of published data to indicate minimal clinically important differences (MCID) for many of these outcome measures. Three such important gait-specific outcome measures include walking velocity, gait profile score (GPS) and the two minute walk test (2MWT).Research questionDetermine the MCIDs for walking velocity, GPS and 2MWT for individuals with lower limb amputation.MethodsWalking velocity and GPS (n = 60), and 2MWT (n = 119) data for individuals with unilateral transfemoral or knee disarticulation were identified retrospectively from a database held at the study centre. An anchor-based method was used with Medicare functional classification level (MFCL) acting as the impairment-related criterion, and a least-squares linear regression approach was used to calculate the gradient required for a change between MFCL levels.ResultsAn increase of 0.21 m/s (95 % CI: 0.13,0.29) for walking velocity, a reduction of 1.7° (95 % CI: -2.449,-1.097) for GPS and an increase of 37.2 m (95 % CI: 28.8,45.5) for 2MWT were found to correspond to an increase in MFCL of one level. Walking velocity, GPS and 2MWT correlated with MFCL with R2 values of 0.333, 0.322 and 0.398 respectively (p < 0.00001). The authors propose that 0.21 m/s for walking velocity, 1.7° for GPS and 37.2 m for 2MWT be used as MCID values for individuals with lower limb amputation.SignificanceThe results of this study can be used to help both researchers and clinicians to objectively evaluate if interventions for individuals with lower limb amputation are effective. 相似文献
58.
《Journal of cranio-maxillo-facial surgery》2020,48(11):1074-1079
IntroductionDiscopexy using resorbable pins is an arthroscopic technique to treat internal derangement of the TMJ, restoring the normal relationship between disc, condyle, and temporal bone. The objective of our study was to assess the 5-year clinical outcome of a series of patients treated with this technique.MethodsA study was conducted on a series of patients who underwent arthroscopic discopexy using resorbable pins between January 2007 and February 2018. All the patients were refractory to conservative treatment and classified as Wilkes stage III. Clinical data were recorded at 1-year, 3-year, and 5-year visits. Pre- and postoperative evaluation parameters were: joint pain (VAS scale), mandibular movements (mm), and articular locking and clicking.ResultsThe study included 33 patients and the technique was performed in 38 joints. Mouth opening increased significantly with each visit after surgery compared with preoperative scores (mean value of 10.65 mm, p < 0.001), with the mean value increasing significantly at the 5-year visit in relation to the mean value obtained at the 3-year visit. Patients reported significant decreases in pain after surgery, obtaining VAS values of under 10 at the 5-year visit (mean improvement of 56.95 points, p < 0.001).ConclusionsDiscopexy using resorbable pins resulted in a good and stable clinical outcome at the long-term follow-up. 相似文献
59.
《中国骨与关节损伤杂志》2015,30(1)
目的 比较经皮椎体后凸成形术(PKP)和微创内固定术的疗效,为治疗单纯性胸腰段椎体压缩骨折选择手术方案提供临床依据.方法 行PKP及微创内固定手术治疗单纯胸腰段椎体压缩骨折59例.PKP组31例,微创内固定术组28例.记录59例术前术后视觉模拟评分法疼痛评分(VAS)、伤椎前缘高度、Cobb角及伤椎高度恢复率.结果 PKP组及微创内固定组在术后VAS评分、Cobb角、伤椎高度、椎体高度恢复率观测指标中,2组间比较差异均有统计学意义(P<0.05).结论 PKP术后止痛效果优于微创内固定组,微创内固定在矫正脊柱后凸畸形及恢复伤椎前缘高度效果上优于PKP组. 相似文献
60.