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51.
目的:分析猝死发生的特点和疾病构成,早期识别高危人群,提高急救水平,降低猝死率,减少医疗纠纷.方法:回顾分析近5年来我院急救中心猝死患者的临床资料,对全部死亡数据进行统计分析.结果:猝死患者以老年人居多,心脏性猝死占首位,脑血管意外居第二位,男性死亡率明显高于女性.结论:心、脑血管患者猝死率最高,应及早发现和控制基础病... 相似文献
52.
Isolation and biological analysis of tumor stem cells from pancreatic adenocarcinoma 总被引:1,自引:0,他引:1
Huang P Wang CY Gou SM Wu HS Liu T Xiong JX 《World journal of gastroenterology : WJG》2008,14(24):3903-3907
AIM: To explore the method of isolation and biological analysis of tumor stem cells from pancreatic adenocarcinoma cell line PANC-1. METHODS: The PANC-1 cells were cultured in Dulbecco modified eagle medium F12 (1:1 volume) (DMEM-F12) supplemented with 20% fetal bovine serum (FBS). Subpopulation cells with properties of tumor stem cells were isolated from pancreatic adenocarcinoma cell line PANC-1 according to the cell surface markers CD44 and CD24 by flow cytometry. The proliferative capability of these cells in vitro were estimated by 3-[4,5-dimehyl-2-thiazolyl]-2, 5-diphenyl- 2H-tetrazolium bromide (MTT) method. And the tumor growth of different subpopulation cells which were injected into the hypodermisof right and left armpit of nude mice was studied, and expression of CD44 and CD24 of the CD44^+CD24^+ cell-formed nodules and PANC-1 cells were detected by avidin-biotin-peroxidase complex (ABC) immunohistochemical staining. RESULTS: The 5.1%-17.5% of sorted PANC-1 cells expressed the cell surface marker CD44, 57.8% -70.1% expressed CD24, only 2.1%-3.5% of cells were CD44^+ CD24^+. Compared with CD44-CD24- cells, CD44^+CD24^+ cells had a lower growth rate in vitro. Implantation of 104 CD44 CD24 cells in nude mice showed no evidenttumor growth at wk 12. In contrast, large tumors were found in nude mice implanted with 103 CD44^+CD24^+ cells at wk 4 (2/8), a 20-fold increase in tumorigenic potential (P 〈 0.05 or P 〈 0.01). There was no obvious histological difference between the cells of the CD44^+CD24^+ cell-formed nodules and PANC-1 cells. CONCLUSION: CD44 and CD24 may be used as the cell surface markers for isolation of pancreatic cancer stem cells from pancreatic adenocarcinoma cell line PANC-1. Subpopulation cells CD44^+CD24^+ have properties of tumor stem cells. Because cancer stem cells are thought to be responsible for tumor initiation and its recurrence after an initial response to chemotherapy, it may be a very promising target for new dr 相似文献
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目的建立复方白及乳膏的质量控制方法。方法观察性状;以a-萘酚硫酸法鉴别白及多糖;以硫酸铜显色法鉴别尿素;以蒽酮染色法测定白及多糖含量;以苯甲醛染色法测定尿素含量。结果复方白及乳膏为白色的乳膏;α-萘酚硫酸鉴别显白及多糖的阳性反应;硫酸铜显色鉴别显尿素的阳性反应;白及多糖在1.88~33.75μg·mL-1范围内呈良好的线性关系,y=0.039X-0.0069(r=0.9998),平均回收率为98.3%,RSD为1.81%,平均含量应为4.0mg·g-1(±10%);尿素在10.0~120.0μg·mL-1范围内呈良好的线性关系,Y=0.0077X+0.0019(r=0.9995),平均回收率为99.1%,RSD为1.49%,平均含量应为0.1g·g-1(±10%)。结论所建立质量控制方法可操作性强,结果重现性好,适用于复方白及乳膏的质量控制。 相似文献
56.
目的:建立头孢曲松钠的XRD和1 H-NMR鉴定方法。方法将头孢曲松钠样品分别用X多晶粉末衍射仪和500MHz高分辨核磁共振谱仪进行测试并得到相应谱图,然后进一步分析和处理。结果 XRD谱图以晶面间距 d(7.96,7.06,5.74,4.82,4.70,4.44,4.19,3.91,3.73,3.16,2.64)为标准对照;1H-NMR谱图以分别位于δ3.5ppm,3.8ppm,6.7ppm和7.1ppm的四个特征单峰:A,B,C和 D,且峰积分面积比 A∶B∶C∶D=3∶3∶1∶2为标准对照。结论上述方法简便易行,无需对谱图中所有的峰进行归属和解析。 相似文献
57.
目的建立糖肾康丸的质量标准。方法采用TLC对糖肾康丸中的黄芪、山茱萸、五味子、黄柏进行定性鉴别。结果定性鉴别方法准确、专属性强。结论该方法简便可行、准确、专属性强,可有效控制糖肾康丸的质量。 相似文献
58.
Anthony H. Lequerica Christian Lucca Nancy D. Chiaravalloti Irene Ward John D. Corrigan 《Archives of physical medicine and rehabilitation》2018,99(9):1811-1817
Objective
To test the feasibility and validity of an online version of an established interview designed to determine a lifetime history of traumatic brain injury (TBI).Design
Cross-sectional.Setting
General community.Participants
A volunteer sample of individuals (N= 265) from the general population across the United States.Interventions
Not applicable.Main Outcome Measure(s)
Online version of the Ohio State University Traumatic Brain Injury Identification Method, Rivermead Postconcussion Symptoms Questionnaire (RPQ), Patient-Reported Outcomes Measurement Information System Cognitive Concerns Scale.Results
The measure was completed by 89.4% of the sample with most participants completing the measure in <8 minutes. After controlling for age, sex, psychiatric history, drug or alcohol history, and history of developmental disability, worst TBI severity was significantly associated with scores on the RPQ, F(2,230)=4.56, P=.011, and having a TBI within the past 2 years was associated with higher scores on the cognitive factor subscale of the RPQ, F(1,75)=7.7, P=.007.Conclusions
The online administration of the Ohio State University Traumatic Brain Injury Identification Method appears to be feasible in the general population. Preliminary validity was demonstrated for the indices of worst TBI severity and time since most recent TBI. 相似文献59.
Kinuyo Chikamatsu Akio Aono Hiroyuki Hata Yuriko Igarashi Akiko Takaki Hiroyuki Yamada Kentaro Sakashita Satoshi Mitarai 《Diagnostic microbiology and infectious disease》2018,90(1):35-39
We evaluated PyroMark Q24 (QIAGEN) pyrosequencing as a method for the identification of mycobacteria, with potential application in clinical practice. Sequence data from the hypervariable region A of the 16S rRNA gene (43 and 35 bp sequences) were obtained using PyroMark Q24, and a similarity search was performed automatically with PyroMark IdentiFire software. Of the 148 mycobacterial type strains tested, 138 (93.2%) were accurately identified to single or clade species level, including complex level. From the remaining 10 strains, 3 (Mycobacterium gilvum, Mycobacterium goodi, and Mycobacterium thermoresistible) showed poor sequencing quality of homopolymers. For 6 other strains (Mycobacterium cosmeticum, Mycobacterium flavescens, Mycobacterium pallens, Mycobacterium hodleri, Mycobacterium xenopi, and Mycobacterium crocinum), the sequences were unreadable from the middle, and Sanger sequencing indicated biallelic site. Finally, a 40 bp sequence for Mycobacterium gordonae could not be obtained despite repeated attempts. PyroMark Q24 provided accurate identification of multiple mycobacterial strains isolated from common clinical settings, but additional gene sequencing is required to distinguish species identified as a group or complex. 相似文献
60.