CD64对新生儿败血症早期诊断的评估

目的观察新生儿败血症时中性粒细胞表面CD64（Fcγ-RⅠ）表达水平,探讨CD64对新生儿败血症早期诊断的意义.方法将48例住院新生儿患儿分为败血症组,局部感染组和非感染组进行血CD64的检测,并与C反应蛋白（CRP）进行比较.结果发现败血症组CD64的水平较局部感染组和非感染组均有显著升高（P＜0.001）,而当CD64取临界值为4 504分子数/细胞,其诊断新生儿败血症的敏感度为90.1%,特异度为100%.结论新生儿败血症时血中性粒细胞表面CD64表达水平明显增加,可考虑作为新生儿败血症早期诊断指标.     

抗白介素-9抗体对BALB/c哮喘小鼠T细胞功能的影响

目的通过比较不同干预措施对小鼠哮喘模型的影响情况,研究其对免疫系统中CD^4＋T辅助细胞2型（Th2细胞）活性的影响,旨在了解外源性抗白介素（IL）-9抗体（Anti-IL-9 Ab）在哮喘中的潜在治疗价值.方法30只BALB/c小鼠随机均分为阳性对照组、Anti-IL-9 Ab组和地塞米松（Dex）组,建立小鼠哮喘模型,采取不同干预措施.比较各组血清、脾和骨髓细胞培养上清液细胞因子的含量.结果Anti-IL-9 Ab组小鼠血清中TH2细胞因子较阳性对照组明显降低（P＜0.01）,Dex组小鼠血清和脾细胞培养上清液中Th2、Th1细胞因子均较阳性对照组明显降低（P＜0.01）,两组骨髓细胞培养上清液细胞因子的含量较阳性对照组均无明显变化.结论Anti-IL-9 Ab和Dex均能明显减轻哮喘小鼠的肺部炎症,在气道建立起新的Th1/Th2平衡.与Dex相比,应用Anti-IL-9 Ab建立的新平衡不会造成因细胞免疫功能受抑引起的一系列不良反应.     

CD44s和Ki-67在脑胶质瘤中的表达及其临床意义

目的:探讨黏附分子CD44s和核相关抗原Ki-67在脑胶质瘤中的表达及其临床意义。方法:采用免疫组织化学SP法对58例不同级别脑胶质瘤标本进行CD44s和Ki-67表达情况检测。结果:CD44s在高级别脑胶质瘤中的阳性表达率为92.31%,显著高于低级别脑胶质瘤(P<0.05);Ki-67在高级别脑胶质瘤中的高表达率为76.92%,亦显著高于低级别脑胶质瘤(P<0.01);CD44s阳性病人术后1年和3年生存率均低于CD44s阴性的病人(P<0.05);CD44s和Ki-67表达呈显著正相关。结论:CD44s与胶质瘤的病理分级、增殖侵袭以及患者预后密切相关。     

CD71和HbF用于流式细胞仪分选母血中的胎儿细胞

目的:比较CD71单染、HbF单染和CD71、HbF双染对单个核细胞的标记情况,同时探讨孕妇外周血中胎儿有核红细胞的含量与孕周的关系。方法:选用红细胞特异性抗体CD71、HbF对正常孕妇外周血和胎儿脐血单个核细胞进行标记,并结合流式细胞仪对有核红细胞进行分选。结果:用CD71和HbF进行双染色标记,正常孕妇和脐血单个核细胞的阳性标记率明显低于CD71和HbF单染色标记(t检验P<0.01)。孕早中期胎儿NRBC比例随孕周的增加呈上升趋势,至21~24周比例最大,约为1.92±0.84×10-2。结论:采用CD71和HbF双染色标记,可提高NRBC的特异性标记和分选纯度,分选胎儿细胞的最佳时间应在孕21~24周。     

卵巢上皮性肿瘤CD105标记的微血管密度的研究

目的探讨卵巢上皮性肿瘤CD105表达及其标记的微血管密度(MVD)与肿瘤发生、发展的关系.方法应用免疫组织化学法检测25例良性肿瘤,10例交界性肿瘤和24例恶性上皮性肿瘤中CD105的表达及MVD.结果59例卵巢上皮性肿瘤中,CD105标记的MVD在恶性肿瘤中显著高于交界性及良性肿瘤(P＜0.01),CD105标记的恶性肿瘤中MVD与肿瘤临床分期、病理分级、患者年龄无统计学相关性(P＞0.05).结论CD105标记的MVD与卵巢上皮性肿瘤恶性进展相关,但不能作为卵巢上皮性恶性肿瘤的预后指标.     

细胞壁缺陷对白喉棒状杆菌Tox基因影响

目的探讨细胞壁缺陷对白喉棒状杆菌毒力基因及其表达的影响。方法以非高渗分离培养法诱导并获得产毒性白喉棒状杆菌稳定L型纯培养物，提取白喉棒状杆菌稳定L型的染色体DNA，用Tox基因特异性引物进行PCR扩增，并进行序列测定和分析。分别采用对流免疫电泳（CIEP）和十二烷基磺酸钠-不连续聚丙烯酰胺凝胶电泳（SDS-PAGE）检测白喉棒状杆菌稳定L型可溶性代谢产物中的白喉毒素蛋白质。结果白喉棒状杆菌在氨苄青霉素作用下可发生细胞壁缺陷而成为L型，该稳定L型的传代培养物可仍然保留同其亲代细菌型一致的Tox基因及其核苷酸序列；但在其可溶性代谢产物中并未检测到白喉毒素蛋白质。结论白喉棒状杆菌稳定L型虽然保留了Tox基因但并不能表达白喉毒素蛋白质，提示细胞壁缺陷可影响Tox基因在宿主菌细胞内的表达。     

妈咪爱对肠道致病菌和条件致病菌及其L型的抑菌作用

目的：探讨妈咪爱对肠道致病菌、条件致病菌及其L型的抑菌作用。方法：采用试管连续稀释法，测定不同浓度的妈咪爱对肠道致病菌、条件致病菌及其L型的光密度值的影响。结果：妈咪爱对大肠埃希菌、福氏志贺菌、伤寒沙门菌、金黄色葡萄球、白假丝酵母菌及其L型皆有不同程度的抑菌作用，且浓度越大，抑菌作用越强，相同浓度的妈咪爱对原型菌的抑菌作用强于细菌L型。结论：妈咪爱是一种疗效很好的微生态制剂，可用于治疗由大肠埃希菌、福氏志贺菌、伤寒沙门菌、金黄色葡萄球菌、白假丝酵母菌及其L型引起的肠道感染。     

Influence of Danshen Injection on airway inflammation and CD4^＋CD25^＋ regulatory T cells of asthmatic rats

Objective： To investigate the influence of Danshen Injection on airway inflammation and CD4^＋CD25^＋ regulatory T cells（CD4^＋CD25^＋ Tr） of asthmatic rats, and elucidate the possible mechanism of Danshen Injection in treatment of asthma. Methods： 30 Wister rats were randomly divided into control group, asthma group and Danshen Injection treated group. Bronchoalveolar lavage fluids （BALF） were collected, and cytology studies were conducted. Lung tissues were obtained and pathologic analyses were done with hematoxylin and eosin stain （HE）. Flow cytometry was used to detect the CD4^＋CD25^＋ Tr ratio in peripheral blood mononuclear cells （PBMCs）. Results： Total cell, the percentage of lymphocytes, neutrophils and eosinophils （Eos） in BALF of Danshen Injection-treated group were lower than that in asthma group （P〈0.05, P〈0.01）. Compared with asthma group, less infiltration of inflammatory cells in lung tissues was observed in Danshen Injection-treated group. CD4^＋CD25^＋ Tr of asthma group was lower than that of control and Danshen Injection treated group （P〈0.05）. Conclusion： Danshen Injection can suppress airway inflammation of asthmatic rats, probably by increasing the number of CD4^＋CD25^＋ Tr.     

Association between CD31 expression and histopathologic features in stage IB squamous cell carcinoma of the cervix

This study was undertaken to evaluate the association between the expression of CD31 in the tumor and the histopathologic findings in patients with carcinoma of the cervix. This study included prospectively 30 women, aged 46.6 +/- 10.7 years, with stage IB squamous cell carcinoma of the cervix submitted to radical hysterectomy from November 2001 to September 2002. Samples from the tumor were taken and immunohistochemically evaluated by a monoclonal antibody for CD31. Clinicopathologic characteristics such as stage, tumor size, grade of differentiation, lymphatic vascular space invasion (LVSI), parametrial involvement, and status of pelvic lymph nodes were also recorded. The clinical stage (FIGO) was IB1 in 22 patients (73.3%) and IB2 in 8 patients (26.7%). The expression of CD31 was significantly associated with tumor size and the presence of LVSI, but not with grade of differentiation and vaginal or parametrial involvement (P= 0.03, P= 0.032, P= 0.352, P= 0.208, and P= 0.242, respectively). On univariate analysis, the presence of pelvic lymph node metastasis was influenced by LVSI (P= 0.003) and CD31 expression (P= 0.032). However, on multivariate analysis, the presence of LVSI (P= 0.007) was the only independent predictor of pelvic lymph node metastasis. The CD31 expression in tumor is significantly associated with LVSI and tumor size in patients with early-stage squamous cell carcinoma of the cervix.     

Expression of CD28 and cytotoxic T lymphocyte antigen 4 at the maternal-fetal interface in women with unexplained pregnancy loss.

OBJECTIVE: To investigate the expression of CD28 and CTLA-4, two costimulatory molecules involved in T-cell activation at the maternal-fetal interface in women with unexplained pregnancy loss. METHODS: A total of 57 women, 39 with unexplained spontaneous abortion (SA) and 18 with unexplained recurrent spontaneous abortion (RSA), were enrolled in the study. A high-resolution spectratyping analysis of fluorescent bands was performed to detect CD28 and CTLA-4 expression in decidual tissues. RESULTS: The mean expression of CTLA-4 mRNA was significantly higher in women with SA than in controls (P<0.05). Moreover, the expression of CTLA-4 was higher in SA patients with genotype AA and phenotype A+ (AA+AG) than in controls (P<0.01). The expression of CTLA-4 was not significantly different in patients with RSA and in controls. The expression of CD28 was significantly decreased in patients with RSA (P<0.01) and SA (P<0.05) compared with controls. The mean ratios of CTLA-4/CD28 were significantly higher in patients with RSA (P<0.01) and SA (P<0.05) than in controls. CONCLUSIONS: This study suggests that an imbalance in CTLA-4/CD28 expression or suppressed T-cell activity at the maternal-fetal interface may confer susceptibility to unexplained pregnancy loss.