Identification of histiocytic reticulum cells by the immunohistochemical demonstration of factor XIII (F-XIIIa) in human lymph nodes

Morphologically and enzyme histochemically distinguishable tissue macrophages and stromal cells of human reactive lymph nodes were characterized by the cytoplasmic presence of the subunit A of factor XIII and by the expression of surface antigenic determinants reacting with monoclonal antibodies directed against monocyte/macrophage populations (Mo 1, Leu M3) and HLA-DR antigens. The distribution of F-XIIIa positive cells was studied on formaldehyde-fixed paraffin-embedded sections with immunoperoxidase techniques. established on cryostat section with double immunofluorescence. Alpha-Naphthyl acetate esterase (ANAE) reaction was The immunophenotype was established on cryostat sections with double immunofluorescence. Alpha-Naphthyl acetate esterase (ANAE) reaction was carried out on these cryostat sections to identify tissue macrophages. The antibody against F-XIIIa detected histiocytes in both intra- and extra-sinusoidal locations which were ANAE+, Mo 1+, Leu M3+ and HLA-DR-. F-XIIIa was also present in fibroblast-like mesenchymal cells with the following phenotypic characteristics: ANAE-, Mo 1+, Leu M3+ and HLA-DR+. The anti F-XIIIa antibody did not stain lymphoid cells, granulocytes, epithelial cells, endothelial cells and mast cells. The immunohistochemical detection of F-XIIIa works on formaldehyde-fixed paraffin-embedded sections. The most promising application seems to be the identification of histiocytes in lymphoid and histiocytic proliferations.     

中学生焦虑与其相关影响因素的综合研究

目的：综合探讨中学生焦虑状况与其相关影响因素的关系。方法：采用中学生焦虑量表、青少年生活事件量表、艾森克人格问卷、中学生父母教养方式量表和应对量表对558名中学生进行问卷调查。结果：(1)中学生整体的焦虑处于轻度水平，52．6％的学生存在不同程度的焦虑问题，男女生在焦虑水平上不存在较为明显的性别差异，年级差异在焦虑水平上表现也不明显。(2)中学生焦虑与生活事件、父母教养方式、人格和应对方式各因子均有十分显著的相关关系，进一步的回归分析结果表明有八个因素对焦虑的预测作用十分明显，具体为不良情绪应对、学习压力、神经质、父放任型教养方式、人际压力、母过分保护和干涉、解决问题和精神质。结论：中学生中存在较为明显的焦虑问题，多个因素对焦虑有预测作用。     

海口市"三级甲等医院"医务人员人格特质调查研究

目的研究试图通过对海口市三甲医院医务人员人格特质的测量与分析,在与全国常模进行对比的情况下,得出海口市三甲医院医务人员人格特质的基本描述,建立医务人员人格特质的常模,为最终科学地建立海南省医学人才人格特质考评体系奠定必要的工作基础,也为海南省医学人才的培养和利用提供一项可行性的管理模式.方法采取分层整群随机抽样方法对海口市五家三甲医院医务人员人格特质进行调查,使用国际著名的人格问卷-加利福尼亚心理调查表CPI作为工具,对海口市三甲医院医务人员人格特质进行调查.结果被调查的海口市三甲医院588名医务人员人格类型分布以Beta型为主,各型分布情况为：Alpha型171人,占29.1%,Beta型302人,占51.4%,Gamma型59人,占10.0%,Delta型56人,占9.5%;在V3自我实现水平上海口市三甲医院医务人员有较高得分,除1例处于第二水平和4例处于第三水平外,其余583例都分别处于第四至第七水平;海口市三甲医院医务人员在20个分量表的测试中,在CS、IN、EM、SC、GI、WB、CM、TO、AI、AC、IE、PY、FX等13个分量表的平均得分上明显高于全国常模组（P〈0.01）,而在DO、SY、SP、SA、SO 5个分量表平均得分上明显低于全国常模组（P〈0.01）.结论海口市三甲医院医务人员各分量表平均得分大部分高于全国常模水平,尤其是在测量成就潜能方面的3个分量表AC、AI、IE平均得分全部明显高于全国常模水平（P〈0.01）,表明海口市三甲医院医务人员较常模有更强烈的成就动机,对智力活动与知识成就有较强的追求,在工作中能更有效地发挥智能,注重办事效率,见识广博,比较适合在激励自由和个人首创精神的场所独立地开展工作,并且能够专心于别人看来较为枯燥的工作.在测量社会价值内化程度的7个分量表中有5个（SC、GI、CM、WB、TO）高于全国常模水平.     

企业员工应对方式与心理健康之间的关系

目的：验证不同应对方式对心理健康的单独与共同影响。方法：对4745名被试用分层回归的方法分析不同应对方式对心理健康的单独影响和交互作用。结论：单独使用“消极应对”的应对方式,对心理健康水平存在不利影响;而单独使用“积极应对”的应对方式能在一定程度上改善心理健康水平。“消极应对”与“积极应对”两种应对方式结合使用能显著改善心理健康水平,比单独使用这两种应对方式对心理健康更有利。     

精神分裂症子女个性行为特征的对照研究

目的 探索材神分裂症子女的个性和行为特征。方法 分别用艾森克个性问卷(EPQ)和Achenbach儿童行为量表(CBCL)，对研究组53例精神分裂症子女，对照组50例正常儿童评定。结果 两组儿童EPQ各项因子分无显著差异，在CBCL分析中，研究组退缩、社交问题和内向因子分显著高于对照组。研究组男童焦虑抑郁，女童躯体主诉分值也显著高于对照组。结论 精神分裂症子女个性无异常。存在有内向，退缩．社交困难等行为问题．应对其早期干预。     

MMPI, personality dysfunction and the dexamethasone suppression test in major depression

In this study we examined the relationship of psychopathology and personality dysfunction to neuroendocrine functioning. MMPI profiles were examined for 30 psychiatric inpatients with major depression who were suppressors (60%) and nonsuppressors (40%) on the dexamethasone suppression test. There were no differences between suppressors and nonsuppressors on any of the MMPI scales or on DSM-III Axis-II diagnosis. When subdivided according to T-score elevations above 70 on MMPI scales 4 and 6, or 4 and 9, 30% of the sample, however, met criteria for personality dysfunction. Furthermore, a significantly higher proportion of suppressors (50%) evidenced personality dysfunction than did the nonsuppressors (8%). This suggests that certain MMPI scales are able to identify a subgroup of depressed patients with personality disturbances who also have a hypothalamic-pituitary-adrenal dysfunction.     

间充质干细胞的免疫调节作用

间充质干细胞(MSC)已成功从动物体尤其骨髓中分离获得.干细胞,具有向多种组织和细胞如骨、脂肪、成软骨细胞等分化的潜能,这种潜能越来越引起人们关注,它可向受损的组织定向迁移以修复受损组织.体外资料研究表明,不管协同刺激信号存在与否,都具有低免疫原性.在体外MSC还具有免疫抑制活性,可抑制同种异体抗原或丝裂霉素刺激的T细胞扩增,阻止细胞毒性T细胞增殖,同时还具有调节炎性细胞的免疫活性使其向抗炎细胞分化的作用.因此,系统了解.MSC的生物学、免疫学特性将为人们深入阐明其免疫调节机制,并将其用于治疗自身免疫性病、组织修复及移植提供依据.     

抗人B因子单克隆抗体的制备及其特性的初步研究

本文以人B因子免疫BALB/c小鼠的脾细胞与SP2/O骨髓瘤细胞融合,获得六株(A_2、B_2、B_7、C_9、F_9、F_(10))分泌抗人B因子单克隆抗体(单抗)的杂交瘤细胞系。用ELISA检测细胞培养上清和小鼠腹水的特异性抗体效价,分别为10~(-2)—10~(-3)和10~(-4)—10~(-5)。抗原阻断试验结果,说明这些单抗是B因子特异的。经取代试验和ACH_(50)抑制试验,提示B因子具有耐热和不耐热二组抗原决定基。A_2和B_2针对不耐热抗原决定基,能抑制补体旁路途径活化;B_7和C_9针对耐热抗原决定基,不影响补体旁路途径活化;F_9和F_(10)则既能与耐热决定基发生反应,也具有抑制补体旁路活化的功能。用固相抗原竞争抑制试验,分析B因子的抗原表位,发现6株单抗可识别4个抗原决定基。这些单抗对B因子结构与功能的研究,B因子在补体旁路活化过程中与有关补体成分之间的相互关系的研究,都是很有意义的。     

重组神经生长因子受体基因在受体缺乏的神经母细胞瘤细胞系中的表达

神经母细胞瘤是儿童期的常见恶性肿瘤之一。临床上,半数以上神经母细胞瘤有明显的N-myc癌基因扩增。神经生长因子(NGF)可使某些神经母细胞瘤细胞分化成神经元样的细胞。但对有N-myc扩增的神经母细胞瘤则可能因NGF受体缺乏而无明显促分化反应。本研究运用重组DNA技术将人NGF受体基因重组到有N-myc扩增,且NGF受体表达很低的神经母细胞瘤系(IMR-32)中,经克隆化培养、筛选,建立了稳定的细胞系—IMR-32/NGFR和对照细胞系IMR-32/NEO。经用抗NGFR的单克隆抗体检测用Flow cytometry技术证实IMR-32/NGFR系中有明显的NGF受体表达,而其母系IMR-32和空病毒对照系IMR-32/NEO则无表达迹象,说明NGF受体表达在IMR-32/NGFR系中是特异的,并能同抗NGFR单克隆抗体特异结合。用Northern B10ting技术亦测得IMR-32/NGFR中NGFR的mRN A明显表达。而其母系IMR-32和对照系IMR-32/NEO则无明显表达。这说明IMR-32/NGFR系中NGFR在mRNA水平上亦是特异的。N-myc和K-ras癌基因在IMR-32、IMR-32/NEO、IMR-32/NGFR三系中无明显变化。在NGF处理后,形态上三系均无明显的分化迹象。但IMR-32/NGFR在神经原纤维轻链的表达上轻度增高。c-fos癌基因的表达见于所有三个细胞系,IMR-32/NGFR略强些。这些说明,在恢复了NGFR基因和表达之后,对N-myc和K-ra     

Personality, manual preference and neuroendocrine reactivity in hirsute subjects

Behavioral and neuroendocrine differences may be postulated in hirsute subjects since central effects of gonadal steroids are well established. We conducted a controlled clinical study with 25 consecutive young hirsute participants compared with 20 consecutive controls. Neuropsychological evaluation included the Minnesota Multiphasic Personality Inventory (MMPI) and the Edinburgh Inventory of Manual Preference (EIMP). Neuroendocrine reactivity was assessed by the adrenocorticotropic hormone (ACTH) and cortisol responses to corticotropin releasing hormone (CRH). Hirsute participants presented a flattened personality profile with lower neurotic triad scores--146 +/- 20 versus 166 +/- 28. Left-hand preference was more common in hirsute participants--4/21 versus 0/20. Decreased ACTH [area under the curve (AUC)--36 +/-2 8 vs. 72 +/- 63 pg/ml h] and cortisol (AUC--18 +/- 4 vs. 25 +/- 10 microg/dl h) responses to CRH were found in the hirsute group. In the hirsute group, higher manual preference scores were associated with lower ACTH responses to CRH, while the opposite association was found in the control group. In the hirsute group, the hyporeactive hypothalamic-pituitary-adrenal (HPA) axis was associated with lower behavior-deviant scores, while in the control group, the hyporeactive HPA axis was associated with more psychopathology. We conclude that personality and HPA axis reactivity are different in hirsute female participants when compared with controls, with a trend for differences regarding handedness. Personality and handedness are differently associated with HPA reactivity. Distinctive features in hirsute participants are probably established very early during ontogenic development.