胰岛素抵抗性高血压大鼠α1肾上腺素受体亚型的改变

目的和方法：用高糖喂养复制胰岛素抵抗性高血压（ｉｎｓｕｌｉｎ－ｒｅｓｉｓｔａｎｔｈｙｐｅｒｔｅｎｓｉｏｎ，ＩＲＨ）大鼠模型，用放射配体结合实验和离体左心房及主动脉收缩功能实验，观察ＩＲＨ大鼠α１肾上腺素受体（α１－ａｄｒｅｎｏｃｅｐｔｏｒ，α１－ＡＲ）及其３种亚型在心脏和主动脉的改变。结果和结论：ＩＲＨ大鼠与同龄ＳＤ大鼠相比：（１）心脏α１－ＡＲ密度下降４８％（Ｐ＜００５），而介导的正性变力效应不变。（２）心脏α１－ＡＲ３种亚型占α１－ＡＲ总数的比例和在介导心脏正性变力效应中的作用表现为α１Ｂ－ＡＲ增加，α１Ｄ－ＡＲ减少，而α１Ａ－ＡＲ不变。（３）介导主动脉收缩的α１－ＡＲ及各亚型对激动剂的敏感性及最大收缩效应均无明显改变。     

Delivery of Antigens to the MHC Class I Pathway Using Bacterial Toxins

Cytotoxic T lymphocytes (CTL) recognize antigens derived from endogenously expressed proteins presented on the cell surface in the context of major histocompatibility complex (MHC) class I molecules. Because CTL are effective in antiviral and antitumor responses, the delivery of antigens to the class I pathway has been the focus of numerous efforts. Generating CTL by immunization with exogenous proteins is often ineffective because these antigens typically enter the MHC class II pathway. This review focuses on the usefulness of bacterial toxins for delivering antigens to the MHC class I pathway. Several toxins naturally translocate into the cytosol, where they mediate their cytopathic effects, and the mechanisms by which this occurs has been elucidated. Molecular characterization of these toxins identified the functional domains and enabled the generation of modified proteins that were no longer toxic but retained the ability to translocate into the cytosol. Thus, these modified toxins could be examined for their ability to carry peptides or whole proteins into the cytosolic processing pathway. Of the toxins studied—diphtheria, pertussis, Pseudomonas, and anthrax—the anthrax toxin appears the most promising in its ability to deliver large protein antigens and its efficiency of translocation.     

献血员HBV携带状况的筛检方法研究

将应用常规RPHA法检查HBsAg阴性的1074份献血员血清再用ELISA和SPRIA法检测。其中又查出HBsAg、HBeAg和抗-HBc-IgM标志,其阳性率分别达5.96％,2.14％和3.35％,HBV携带率竟高达9.68％。说明用常规RPHA法筛选出的HBsAg阴性献血员仍有传播乙型肝炎的危险。用ELISA法并联检测HBsAg和抗-HBc-IgM,在RPHA法检查HBsAg阴性的献血员中、其阳性率为8.29％,敏感度达85.58％,因而这种并联筛检方法可成为一种切实可行的筛检方法。     

Multiple sites of action of ( +)-3-(3-hydroxyphenyl)-N-(1-propyl)piperidine (( +)-3PPP) in blood vessels

Functional effects of the σ ligand, (+)-3-(3-hydroxyphenyl)-N-(1-propyl)piperidine ((+)-3PPP), were explored in perfused rat tail and rabbit ear arteries in vitro. In the rat tail artery (+)-3PPP inhibited contractile responses to adrenergic nerve stimulation, an effect which was reversed to potentiation by the dopamine D₂ receptor antagonist sulpiride. In the rabbit ear artery, however, (+)-3PPP potentiated contractile responses to nerve stimulation, an effect which was unchanged by sulpiride. In the rat tail artery, blockade of norepinephrine uptake by cocaine and deoxycorticosterone in the presence of sulpiride revealed two additional actions of (+)-3PPP. First, an inhibitory action on the monoamine uptake site was confirmed by direct measurement of [³H]norepmephrine accumulation. Second, at higher concentrations, an action to inhibit contractile responses to adrenergic nerve stimulation was manifested at a still unidentified site. These studies demonstrate that the observed functional effect of (+)-3PPP results from its combined actions on three individual sites with the net effect dependent on the relative densities of these different receptor sites in each type of vessel.     

细胞间粘附分子在翼状胬肉中的表达及意义

目的　研究翼状胬肉组织中细胞间粘附分子1(ICAM-1)的表达水平,探讨ICAM-1在翼状胬肉病理过程中的作用。方法　采用酶联免疫吸附试验法(ELISA)检测28例翼状胬肉患者组织匀浆中的ICAM-1含量,并与正常人结膜组织进行对照。结果　翼状胬肉组织匀浆中ICAM-1含量明显高于正常对照组(P<0.01),进行期组的ICAM-1含量明显高于静止期组(P<0.05)。结论　在翼状胬肉组织中存在ICAM-1异常表达,可能是翼状胬肉发病的重要原因之一。     

Infant rat separation is a sensitive test for novel anxiolytics

1. Rat pups emit ultrasonic calls during brief episodes of social separation. These calls have been variously described as “distress” calls and may be related to the separation cries expressed by the young of many mammalian species.

2. Ultrasonic call of rat pups are modulated by environmental stimuli such as ambient temperature, olfactory and tactile stimuli associated with the nest.

3. Calls are also sensitive to a variety of purported anxiolytic and anxiogenic drugs, including the benzodiazepines, serotonin agonists, and ligands at the NMDA-glycine receptor complex.

4. In addition to providing a simple test for the anxiolytic properties of drugs, this model may also provide new insights about the development and neurobiology of anxiety.     

表达屋尘螨Ⅰ类变应原Der p 1的基因工程菌的发酵条件探讨

目的 探讨发酵条件对大肠杆菌工程菌表达重组屋尘螨Ⅰ类变应原(Der p 1)的影响.方法 应用摇瓶发酵试验观察不同诱导时间和不同诱导时机对Der p 1表达量的影响.结果 (1)Der p 1基因在E.coli BL21(pBVDP8)菌密度A600 nm0.6左右时,42℃最佳诱导时间为4 h.(2)工程菌E.coli BL21(pBVDP8)在30℃培养至A600 nm为0.5～0.7时,42℃诱导4 h Der p 1表达量最高.结论 确定了最适诱导时间和诱导时机,初步形成了一套高表达Der p 1的发酵技术.