5-Fu抑制兔眼滤过道成纤维细胞的AgNORs表达

目的　应用细胞增殖指标AgNORs,研究兔眼滤过道成纤维细胞 (Fb)增殖规律及 5 Fu的抗增殖作用。方法　取兔眼滤过道切片作AgNORs及HE染色 ,比较 5 Fu眼和对照眼FbAgNORs染色颗粒数量。结果　对照眼滤过道的咬切口、巩膜瓣下、结膜瓣下均有Fb增殖 ,且增殖活性第 7天最高 ,第 3 0天最低。 5 Fu眼FbAgNORs颗粒数显著低于对照眼 ,5 Fu抑制率为 44.9%～ 73 .0 %。结论　 5 Fu可以有效抑制咬切口、巩膜瓣下、结膜瓣下Fb的增殖活性     

An Experimental Study on Homoharringtonine Liposome and Glaucoma Filtration Surgery

Purpose: To observe the inhibitory action of homoharringtonine liposome during the healing process of wounds in the filtering sitesMethods: posterior sclerectomies were performed in 14 rabbits. Postoperatively one eye of each rabbit received subconjunctival injections of HH liposome and fellow eye received saline injection in a randomized masked fashion.Results; Fourteen days after operation the IOP of experimental eyes reduced significantly (P < 0.01) as compared with the controlled eyes, and the number of remaining filtering blebs increased noticeably (P< 0.05). Pathohistological examination revealed that the number of fibroblasts per square micron in the filtering sites and the thickness of the scars in the center of the filtering sites of the experimental eyes were less than those of the controlled eyes. No serious ocular toxic and side effects were found.Conclusion : This experiment suggests that homoharringtonine liposome can markedly inhibit the scar formation of filtering sites after glaucoma fi     

内眼手术前,后结膜囊细菌学研究

目的:探索眼科内眼手术前后结膜囊细菌学变化特点及临床眼科用药效果。方法:133眼需要行内眼手术的连续病例包括白内障手术68眼、青光眼手术39眼、视网膜脱离手术20眼、玻璃体切割术6眼。用133眼内眼手术病人手术前后的结膜刮片及68眼白内障手术术毕的前房冲洗液作细菌培养,并作药敏试验。结果:133眼内眼手术患者入院时结膜刮片作细菌培养有61眼(45.85％)培养出细菌,其中金黄色葡萄球菌23眼、表皮葡萄球菌25眼、G~ 杆菌13眼;手术消毒前结膜囊刮片有8眼(6％)仍培养出细菌,其中金黄色葡萄球菌3眼,表皮葡萄球菌5眼;68眼白内障手术患者前房冲洗液中仅1眼(1.5％)培养出细菌,为表皮葡萄球菌;术毕时结膜刮片4眼(3％)培养出细菌,所培养出来的细菌均对妥布霉素、庆大霉素、先锋Ⅵ、万古霉素和利福平敏感,而对四环素、氯霉素、红霉素、氧氟沙星耐药。结论:肉眼手术前应用有效抗菌素至为重要,可预防眼内炎的发生,但不可轻视手术无菌操作。眼科学报1999;15:267-269。     

Strabismus and diplopia as complications after cataract surgery with IOL implantation

Summary In our Department of Orthoptics we have seen an increasing number of patients suffering from diplopia after cataract surgery with IOL implantation. Between 1993 and 1997 the total number of patients with this problem was 24 (2.7 % of all patients, mean age 71 years, age range 38–88). We addressed the question of whether there is a common pattern of motility dysfunction. Methods: After evaluation of the clinical history and the basic ophthalmological findings the following parameters were examined: binocular function (Bagolini test), squint angles (Maddox cross), ocular motility. Results: The 24 patients could be divided up into three groups. Group 1 consisted of 9 patients (mean age 82 years, range 64–88) who complained about diplopia because of strabismus incomitans with vertical deviation and restricted motility on the first day after surgery. In 8 of the 9 patients strabismus surgery was done. Group II consisted of 10 patients (mean age 66 years, range 38–77) who noticed diplopia and strabismus within 7 days after surgery. We found various kinds of heterotropia. Seven of these patients were operated on and two had a prism correction. Group III consisted of 5 patients (mean age 67 years, range 61–78). Their already known strabismus paralyticus or concomitans deteriorated, leading to diplopia in some cases. All patients in this group were operated on. Discussion: For group I we believe that retro-, para- or peribulbar anesthesia caused the motility dysfunction. In groups II and III it is unlikely that local anesthesia had a causative role. The prolonged disruption of binocular vision and the abrupt change in the sensory situation after the cataract operation with lens implantation may be the leading causes for strabismus or deterioration of a preexisting strabism, respectively. Conclusions: These patients need a subtil meticulous diagnostic work-up and follow-up because of the possibility of early surgical therapy, which has a good prognosis. Evaluation of binocular vision and eye movements prior to cataract surgery appears to be helpful for later strabismic surgery.      

后巩膜加固术对视网膜振荡电位的影响

目的　为了更进一步地了解后巩膜加固术治疗高度进行性近视的疗效和机理。方法　采用日本产Ｎｅｕｒｏｐａｃｋ Ｆｏｕｒ ４１０４ Ｋ 电生理仪,对２６ 例５２ 眼高度进行性近视行后巩膜加固术前、术后视网膜振荡电位进行定量分析。结果　术前、术后振荡电位总振幅经统计学处理有非常显著性差异,波的潜伏期无显著性差异。结论　本文认为后巩膜加固术可改善视网膜血液循环,并对机理进行了探讨     

不同手术切口对白内障术后视力和屈光力的影响

目的　评价白内障不同的手术切口对术后视力恢复的影响。方法　对１８６ 只眼老年性、并发性、发育性和无角膜瘢痕的外伤性白内障行ＥＣＣＥ（ＣＣＣ） 加ＩＯＬ。切口为１１ ｍ ｍ ,隧道６ ｍ ｍ 及反眉状５ ．５ ｍ ｍ 巩膜切口,手术后１ ｗｋ ,１ ｍ ｏ ,３ ｍ ｏ 进行视力和屈光检查与比较。结果　随着手术切口的缩小和手术切口的改进,角膜散光明显减少,视力明显提高。结论　提示反眉状隧道式白内障摘出术ＩＯＬ 术具有有效控制术后散光,全面重建视功能的优点     

抗青光眼术在无晶体眼和人工晶体眼的疗效

探讨发生于无晶体眼和人工晶体眼的青光眼的有效的手术治疗方法。方法：回顾分析１９９２年１１月～１９９７年６月我院４５例４５眼发生于无晶体眼和人工晶体眼的青光眼的住院病例的临床资料。手术方法包括小梁切除术、睫状体冷冻术、青光眼减压阀植入引流术以及其他手术。结果：青光眼减压阀植入引流术控制眼内压优于睫状体冷冻术和小梁切除术,术后合并使用降眼压药物少于睫状体冷冻术后。结论：发生于无晶体眼和人工晶体眼的青光眼的治疗需根据临床情况考虑,青光眼减压阀植入引流术有较好的疗效     

LASIK前后非接触眼压计测量结果的研究

目的：研究激光原位角膜磨镶术（LASIK）手术前后非接触眼压计（NCT）测量值的改变,及其与屈光度矫正值和术中角膜切削厚度之间的关系,方法：随机选择112眼接受LASIK治疗的高度近视患者,排除青光眼或高眼压症,于术前、术后3、6、12个月作非接触眼压测量,对术前、术后的非接触眼压测量值作u检验,并对眼压改变值与预期屈光度矫正值和术中角膜切削厚度作相关性分析,结果：①手术前后非接触眼压测量值的改变差异有显著性意义（P＜0.001）,术后一年平均眼压测量值下降0.931±0.293kPa;③术后一年眼压下降值与预期屈光度矫正值存在统计学上的相关性(r=0.1942,P＜0．05);术后一年眼压下降值与术中角膜切削厚度亦存在统计学上的相关性(r=0.2577,P＜0.001）,结论：LASIK术后不能按传统正常值衡量非接触眼压计测量结果,否则可能延误类固醇性青光眼的早期诊断,激光切削石角膜厚度变薄是导致手术后非接触眼压计测量结果下降的直接原因。     

对外科手术中意外低体温影响因素的观察研究

对３０例神经外科择期手术体温正常患者术中影响体温变化的因素进行观察。在国麻醉期持续监测体温、室温及相对湿度,记录液体输注量及尿量。结果显示,当室温≤２４℃、手术时间＞ ５小时、输注室温下人液量≥（６５１±１２１）ｍｌ／ｈ时,可导致意外低体温。所以,麻醉中体温监测是必不可少的。     

Timing of surgery for common pediatric surgical conditions

Depending upon various factors the surgical procedures in childhood are divided into three groups—immediate, intermediate and elective. The timing of surgery is probably the most important factor governing the outcome of surgical correction in pediatric surgery. With continuing research and clinical experience our understanding of the various conditions have improved and with that has come a change in the optimum timing of many surgical procedures. This paper highlights the best timing of surgery for some of the commoner pediatric surgical procedures and the reasons behind these so that the children may be referred to pediatric surgeons in time.