Therapeutic effects of liposomal adriamycin in combination with tumor necrosis factor-alpha.

Liposomes as drug carriers in cancer chemotherapy have attracted considerable interest. To enhance the therapeutic effect of Adriamycin entrapped in liposomes (Lip-ADM) on human solid tumors, we investigated the therapeutic effects of Lip-ADM in combination with recombinant human tumor necrosis factor-alpha (rTNF-alpha), which is known to have specific effects on tumor vasculature. rTNF-alpha or saline solution was injected intravenously into nude mice bearing a human colon cancer strain, HC-1, at 1 hour before intravenous administration of Lip-ADM. The significant therapeutic effect of Lip-ADM in combination with rTNF-alpha was demonstrated by the evaluation with tumor growth curve and the actual tumor weights, in comparison with groups of mice treated with saline solution, rTNF-alpha alone, or with a Lip-ADM after saline. Levels of Adriamycin in tumor tissue in the Lip-ADM in combination with rTNF-alpha-treated group were higher than those in Lip-ADM with saline solution-treated group.     

Optimization of parameter values for complex pulse sequences by simulated annealing: Application to 3D MP-RAGE imaging of the brain

A number of pulse sequence techniques, including magnetization-prepared gradient echo (MP-GRE), segmented GRE, and hybrid RARE, employ a relatively large number of variable pulse sequence parameters and acquire the image data during a transient signal evolution. These sequences have recently been proposed and/or used for clinical applications in the brain, spine, liver, and coronary arteries. Thus, the need for a method of deriving optimal pulse sequence parameter values for this class of sequences now exists. Due to the complexity of these sequences, conventional optimization approaches, such as applying differential calculus to signal difference equations, are inadequate. We have developed a general framework for adapting the simulated annealing algorithm to pulse sequence parameter value optimization, and applied this framework to the specific case of optimizing the white matter-gray matter signal difference for a T₁-weighted variable flip angle 3D MP-RAGE sequence. Using our algorithm, the values of 35 sequence parameters, including the magnetization-preparation RF pulse flip angle and delay time, 32 flip angles in the variable flip angle gradient-echo acquisition sequence, and the magnetization recovery time, were derived. Optimized 3D MP-RAGE achieved up to a 130% increase in white matter-gray matter signal difference compared with optimized 3D RF-spoiled FLASH with the same total acquisition time. The simulated annealing approach was effective at deriving optimal parameter values for a specific 3D MP-RAGE imaging objective, and may be useful for other imaging objectives and sequences in this general class.     

不同时辰刺激大鼠的交叉上核对血浆中皮质酮含量的影响

应用拉丁方设计，在不同体重组的大鼠，于不同时辰，对交叉上核采取不同强度的刺激，用放射免疫法测定外周血中皮质酮的含量。其结果显示：各体重组间无显著性差异，不同时辰血浆皮质酮的平均含量各组呈现基本相同的节律性变化，无论何时辰刺激交叉上核使之兴奋，均可显著提高血浆皮质酮的含量。完全损毁交叉上核后７２小时血浆皮质酮含量和正常对照组无显著性差异，且其节律性也没有出现明显变化。     

不同分娩方式对早产儿影响的临床研究

目的：探讨妊娠小于34周早产分娩方式的选择。方法：妊娠小于34周早产患者126例分为剖宫产组67例和阴道分娩组59例，比较不同分娩方式对分娩结局的影响。结果：孕28—30＋6周时，阴道分娩组的新生儿死亡率明显高于剖宫产组（P〈0．05），其他两个孕周期间的两组新生儿死亡率比较无显著差异。阴道分娩组和剖宫产组的新生儿的死亡数随着出生体重的逐渐增加而不断减少，新生儿体重小于1500g时，两种分娩方式的死亡率之间比较存在显著性差异。结论：早产的主要原因是妊娠并发症，降低新生儿不良结局的根本措施还应延长孕龄，提高新生儿生存能力。     

基于多评价指标的萎胃宁浓缩丸提取工艺研究

目的优选萎胃宁浓缩丸制剂的最佳提取工艺。方法以芍药苷提取量、正丁醇浸出物、乙酸乙酯浸出物为指标，采用均匀设计法筛选最佳提取工艺。结果乙醇浓度60％，提取次数2次，溶剂用量16倍，每次提取时间65min为最佳提取工艺。结论所得提取工艺稳定性好，可用于萎胃宁浓缩丸的制备。     

成都市中老年健身女性体成分指标与骨密度和骨代谢指标的相关性研究

目的探讨体重、体重指数(BMI)等体成分指标对中老年健身运动女性骨密度的影响及体成分指标与骨代谢指标、骨密度指标的关系。方法94例成都市城区健身运动女性根据BMI不同分为三组:低体重组(BMI≤20kg/m2)、正常体重组(20kg/m225kg/m2),采用Osteospace超声骨密度仪测定跟骨的BUA、SOS、STI骨密度指标;全自动生化分析仪测定血清AKP含量;应用放射免疫法测定血清hCT、BGP、IL-6、E2、TNF含量。应用方差分析和偏相关方法进行统计学处理。结果不同BMI组的体重、体重指数、瘦体重和体脂百分比差异显著,低体重组T-score与正常体重组、超重组比较有极显著差异;低体重组SOS、STI骨密度指标显著低于正常体重组;BUA、SOS、STI骨密度指标与体重、体重指数、瘦体重和体脂百分比呈正相关,与hCT、IL-6、TNF、BGP、AKP呈正相关,与E2呈负相关。低体重组骨量减少、骨质疏松发生率最高。结论体重、体重指数等体成分指标是影响中老年健身运动女性BMD的重要因素,保持体重有利于防止骨丢失和预防骨质疏松发生。     

极低出生体重儿两种喂养效果观察

目的:探讨极低出生体重儿(VLBW)的早期微量持续鼻饲喂养(CNG)与间断鼻饲喂养(ING)的喂养耐受性及喂养效果.方法:将60例VLBW进行随机分组,CNG组起始奶量1-2ml/h,以后泵速增加1-2ml/h.ING组奶量以1-2ml/kg开始, 以后每天增加1-2ml/kg/次.所有VLBW均同时进行部分静脉营养,直至达到完全胃肠道喂养时间(FEF).对比两组喂养不耐受性、达到FEF、停止静脉补液时间及黄疸持续时间.结果:CNG组较ING组患儿发生腹胀、呕吐者明显减少(P＜0.05),黄疸持续时间、吸吮动作出现时间及住院时间较对照组明显缩短(P＜0.05),但两组体重增长情况统计学无差异(P＞0.05).结论:CNG更适合VLBW的早期微量喂养.     

低分子肝素钙联合辛伐他汀治疗不稳定型心绞痛疗效观察

目的探讨低分子肝素联合辛伐他汀治疗不稳定型心绞痛的疗效。方法将100例不稳定型心绞痛患者随机分为对照组50例和观察组50例。对照组仅采取常规治疗（硝酸酯类、β-受体阻滞剂、钙拮抗剂、阿司匹林等），观察组在常规治疗的基础上加用低分子肝素和辛伐他汀，观察比较各组的疗效。结果观察组总有效率为90．0％，对照组总有效率为60．0％，两组疗效比较差异有显著性意义（P〈0．05）。结论低分子肝素联合辛伐他汀治疗不稳定型心绞痛取得满意的疗效。     

乳酸菌SK1.002产L-阿拉伯糖异构酶培养基优化及发酵条件

L-阿拉伯糖异构酶能将D-半乳糖异构成D-塔格糖。通过单因素试验和快速登高法对乳酸菌SK1.002产L-阿拉伯糖异构酶的培养基进行优化,确定发酵优化条件为（组分g/L）：麦芽糊精28,酵母膏10,玉米粉浆22,无水乙酸钠10,K3PO40.2,NaCl 0.01,FeSO4.7H2O 0.01,Mg-SO4.7H2O 0.2,MnSO4.2H2O 0.05,L-阿拉伯糖2.5。发酵初始pH 8.4,培养温度37℃,接种体积分数3%,培养时间12 h。在此发酵条件下,酶活达到了7.28 U/mL。     

低分子肝素对急性脑梗塞的影响

为了探讨低分子肝素（ＬＭＷＨ）在脑梗塞应用中的安全性及实用性，采用随机单盲对照方法，对３９例急性脑梗塞病人，予ＬＭＷＨ治疗，并与低分子右旋糖酐治疗进行比较。结果显示：ＬＭＷＨ组对不同程度患者的神经功能恢复程度明显优于低右组（Ｐ＜００１），ＬＭＷＨ组头颅ＣＴ扫描脑梗塞体积缩小的范围比对照组明显（Ｐ＜００５），ＬＭＷＨ不减少血小板数量及纤维蛋白量，不引起出血，可改善血液流变学，降低微循环总积分值。总有效率８７１８％。