脑梗死患者组织因子途径抑制物C-399T多态性

组织因子途径抑制物（TFPI）是外源性凝血途径起始步骤的生理性抗凝物质，在调节血栓形成、抑制溶栓后血管再闭塞等方面具有重要作用。近年研究表明TFPIC-399T基因多态性与静脉血栓形成无关，但与动脉血栓性疾病的关系仍有争论，且不同人种TFPIC-399T的分布频率不同。为了研究TFPIC-399T多态性在中国人群中的分布频率及其与脑梗死（Cerebral Infarction，CI）之间的可能联系，我们分析了部分CI患者及正常人的TFPIC-399T等位基因的变异情况及血浆总TFPI抗原浓度，现报道如下。     

不同频率振动应变对成骨细胞增殖及分化能力的影响

目的 观察不同频率振动应变(vibration strain)对体外培养成骨细胞细胞周期、增殖能力及分化的影响,确定最佳振动频率.方法 应用自制复合振动仪,将振动强度0.5g(g为加速度),不同频段3～10、15～30、25～45、50～80和80～100Hz振动应变分别作用于成骨细胞,振动应变加载后分别检测细胞周期、细胞增殖能力(MTT法)及碱性磷酸酶(alkaline phosphatase, ALP)活性的变化.结果 15～30和25～45Hz频段诱导成骨细胞周期循环(P<0.01),促进细胞增殖(P<0.01),增强ALP活性(P<0.01);80～100和3～10Hz频段抑制细胞周期循环(P<0.01);80～100Hz频段抑制细胞增殖(P<0.01);50～80和80～100Hz频段抑制ALP活性(P<0.01).结论 不同频率振动应变对成骨细胞周期、增殖能力、ALP活性均有影响,振动应变促进成骨细胞增殖及分化的最佳频率为15～45Hz.     

载脂蛋白A1、B基因多态性对非创伤性股骨头坏死发生的影响

目的:探讨载脂蛋白A1、B基因多态性对非刨伤性股骨头坏死(avascular necrosis of the femoral head,ANFH)发生的影响.方法:应用聚合酶链反应对中国北方汉族143例ANFH患者和92例正常人分别扩增含Apo AI基因启动子-75 bp和第一内舍子 83 bp及Apo B基因Eco RI、XbaI和3-VNTR的DNA片段,限制性内切酶酶切扩增产物,琼脂糖凝胶电泳分离基因多态性.结果:Apo A1基因启动子-75 bp处,ANFH患者中A/A基因型频率明显高于正常组(P＜0.01),而G/A基因型频率明显低于正常组(P＜0.01).Apo AI内舍子 83 bp位点,Apo B基因Eco RI、Xba I位点和3-VNTR区域ANFH患者组和正常组基因型及等位基因频率分布无统计学差异.结论:Apo A1基因启动子区域-75bp位点A/A型可能是非创伤性股骨头坏死易感基因之一,但未能发现Apo A1第一内舍子 83 bp位点及Apo B基因Eco RI、XbaI和3-VNTR位点多态性与非创伤性股骨头坏死发生有明显的关系.     

连台手术病人的安全护理

护理安全是指在实施护理的全过程中,患者不发生法律和法定的规章制度允许范围以外的心理、机体结构或功能上的损害、障碍或死亡[1].手术室护理安全是护理管理的重点,是护理质量的重要标志之一.随着现代手术室的使用,连台手术频率和手术强度也有随之增加.紧张繁忙的护理工作中,存在着诸多安全隐患,稍有不慎,就有可能造成严重后果.为加强管理,防止或减少护理纠纷的发生,我院结合实际情况,分析了连台手术所存在的各种风险因素,并制定相应的护理对策,现总结如下:     

Evaluating therapeutic outcome in epileptic patients using the changes of interval and duration

BACKGROUND: Seizure frequency is in abnormal distribution, and it is not enough to express the trend of concentration using means, and its median loses a lot of information, thus it lacks of a standard for evaluating the therapeutic effects based on seizure frequency. OBJECTIVE: To establish a method for evaluating the therapeutic effects on anti-epileptic drugs using changes of interval and duration of seizure. DESIGN: A prospective cohort study. SETTING: Zhumadian Psychiatric Hospital. PARTICIPANTS: Outpatients and inpatients suffering from epilepsy attending firstly visited Zhumadian Psychiatric Hospital from June 2001 to June 2002 were enrolled. They were diagnosed as epileptic according to the International Classification of Epileptic Seizure by International League Against Epilepsy (1981) based on the clinical history, physical examination, and investigations. The interval time was no more than 6 months. Informed consent was obtained from all the subjects, and the study was approved by the hospital ethical committee. METHODS: ① For the first visit and each follow-up, the following data were recorded, including general demographic information, seizure type, the date and time of ictus, the duration of ictus, and inducement or situation related, according to which the following indexes could be calculated, including seizure styles, interval, duration, cluster frequency and cluster duration. The information from the first review was noted as annals A. The second interview was taken at the end of the evaluating period; the information from the second review was noted as annals B. The third interview was taken within two weeks after the second one; the information from the third review was noted as annals C. The annals B or the annals C were respectively compared with the annals A in the light of the same types or the same styles of the same patient. Summation of the scores of interval change and duration change of the same type or the same style and 5 of basic score was the score of a corresponding seizure type or a corresponding style of one patient. In order to test its reliability and validity, the score of change of frequency or duration plus 5 scores respectively was the score of frequency or duration. ② Reliability and validity were tested by calculating corresponding correlation coefficient with SPSS 11.0. ROC curve was used for developing diagnostic criterion of predicting therapeutic effects with SPSS 11.0. MAIN OUTCOME MEASURES: ① Reliability and validity; ② Diagnostic criterion for predicting therapeutic effects one year later. RESULTS: Totally 28 patients were involved in the final analysis of results. ① Reliability and validity were high: rinter-rater=0.98, rtest-retest=0.99, rconstruct validity=0.83. ② A total score > 6 was the optimal diagnostic criteria for predicting therapeutic effects one year later, in other words, a patient who scored more than 6 at the evaluating period may be seizure-free one year later. CONCLUSION: It is a potential tool for evaluating epileptic therapeutic outcome, and it can be diffusely used in interrelated fields after being further validated.     

平和体质女性的五音频率与年龄的相关性研究

目的:采用二十五音分析仪,对平和体质女性的声音频率特征进行探讨。方法:按《黄帝内经&#183;素问》的女性生理年龄特征划分法,以“五七“年龄为界,将观察对象分为年轻组(20～35岁)和年长组(36～50岁)。运用二十五音分析仪对两组女性的声音频率进行检测。结果:平和体质女性的声音频率随着年龄的增长偏向于角音;年轻组的声音频率高峰在羽音区,徵音、羽音亦增多;两组的声音频率比较有统计学差异。结论:女性平和体质者的声音频率具有一定的特征,并随着年龄的增长具有规律性的变化。     

子宫全切术对性生活的影响

为探讨不同术式子宫全切对性生活的影响 ,本文对 2 0 3例患者进行了分析 ,现将结果报告如下。对象随机选取 2 0 0 1年 1月至 2 0 0 2年 12月在我院因良性疾病行子宫全切术加一侧附件切除术的病人 2 0 3例 ,平均年龄 42 .3 5岁 ( 2 5～ 5 0岁 )将其中未婚 1例 ,术前离婚 5例排除后 ,入选病人 197例。其中因子宫肌瘤手术者 14 5例 ,子宫腺肌瘤者 45例 ,附件及其他原因者 7例。方法在医院驻地社区通过问卷式调查 ,在病人术后半年左右进行逐一随访。调查内容包括 :手术前后的性欲望、性生活频率、性质量满意度 ,对子宫全切术的理解 ,对未来夫妻…     

定量脑电图适宜采样时间的选取

定量分析脑电图(EEG)时的采样时间以多么长为宜,众说不一。笔者通过分析不同时间枕区主频段的频率和功率,探讨定量EEG最佳采样时间。 1 对象和方法 受试者38人,均为健康者,男29人,女9人。年龄18～49岁。应用日本光电ME-17脑电仪与国产DYD-500型脑地形图仪。按常规EEG检查方法,采集单极导联,采样总时间160s,采样频率100Hz,分析精度±0.15 Hz。选用6s、30s、60s、90s、120s、150s 6种不同时间段的脑电作EEG枕区功率谱主频段的频率和功率分析。     

用STR对两例DMD高危家系的产前诊断及携带者检出

本文利用DMD基因位点内4个STR部位基因扩增后通过多态性分析对两例非基因缺失型DMD高危家系进行了产前诊断和携带者检出。内含子49内(CA)n杂合频率最高达0.933。这四个部位STR 特异性高,提供的遗传信息量多,利用STR—PCR后多态性分析较以往的RFLPs连锁分析更有价值,该方法将成为一种极有前途的适用于临床产前诊断和携带者检出的新手段。     

安徽人群St14/Taq I RFLPs的研究

作者为寻找FⅧ基因的遗传标记,应用Southern印迹法对安徽人群的Stl4/Taq I RFLPs进行研究。结果表明:在59条无血缘关系的X染色体中,第1、2二个多态系统的等位基因频率分别为:1(5%),4(5%),5~6(15%),7(27%),8(47%),α(47%),β(53%)。总的杂合频率为82%。提示:此RFLPs对甲型血友病的连锁分析非常有用。