改良以聚羟基乙酸为支架同种异体组织工程化塑形软骨的构建

OBJECTIVE: To improve the method for constructing allogeneic molded cartilage by means of tissue engineering techniques. METHODS: The chondrocytes from the rib and articular cartilage of infant rabbits were harvested by type II collagenase digestion, followed by in vitro cell culture for 3 to 4 passages. The chondrocytes were then prepared into cell suspension and seeded onto C -and O -shaped pre-molded polyglycolic acid (PGA) scaffolds form chondrocyte-PGA composites, which were subsequently cultured in vitro for 7 to 10 d before implanted subcutaneously into adult rabbits. Improvement was made upon conventional shaping and implantation procedures. Morphological observation and cartilage regeneration assessment were conducted at different time points following the implantation, in comparison with the observation by conventional shaping and implantation methods. RESULTS: During in vitro cell culture, the rate of viable chondrocytes in the final cell suspension was (92+/-2)% after well-controlled prolongation of digestion trypsin, similar to the viable cell rate (93+/-2) % by traditional procedures (P>0.05). Gross observation found milk-white, newly generated cartilage which had good flexibility 4 weeks after implantation, and after 8 weeks and later, the cartilage took on the color of porcelain-white. Histological examination showed a few inflammatory cells around the newly generated immature cartilage 4 weeks after implantation, and the inflammation abated when the newly generated cartilage acquired similar histological properties to that of the original cartilage 8 weeks postoperatively and later. After 16 weeks, no blood vessel or capillaries were visible within the new cartilage. CONCLUSION: The chondrocyte viability is not affected when the cells are treated with well-controlled prolonged digestion with trypsin during in vitro cell culture. Improved PGA scaffolds shaping and the implantation procedure facilitate the regeneration of the cartilage after the implantation of the composites.     

臀位孕期矫治的临床探讨

1 资料与方法1.1 资料 从1990年6月至1995年12月,我院产科门诊孕期检查为臀位,孕28～37周采用综合治疗1401例设甲组,矫治无效,排除禁忌症,术前B超检查,口服硫酸舒喘灵4.8mg,行改良式臀位外侧转术105例为乙组。1.2 方法1.2.1 综合治疗 艾炙、姜炙双足至阴穴,膝胸卧位,甩臀法等,单用或两法同用,7天为1疗程。1.2.2 改良臀位外倒转术 术前B超了解臀位分类、胎方位、羊水量,胎盘附着、脐带有无缠绕,排除禁忌症,术前30min至1h口服硫酸舒喘灵4.8mg,排尿后仰卧床上,常规产检后,稍抬高头部,双腿屈曲使腹壁松弛,术者一手从耻上上托胎臀使其离开骨盆入口平面,另一手握住胎头向胎儿腹侧俯曲下推,两手交替托不放开。胎位转成横位时可稍停,利用胎儿自身活动轻推胎头可转为头位,其间可监护胎心2     

红斑、丘疹及鳞屑性皮肤病

20063143白介素20 mRNA在寻常性银屑病患者皮损的表达/翟宁(中国医大一附院皮肤科),张庆瑞,李波…∥中华皮肤科杂志.-2006,39(7).-409~410采用原位杂交技术检测35例寻常性银屑病患者皮损和20例正常人皮肤组织中IL-20 mRNA表达。结果显示正常人皮肤组织中的阳性率为10.0%,着色强度为( );寻常性银屑病皮损组织中的阳性率为100%,且绝大部分着色强度为( ~ )。提示IL-20 mRNA在寻常性银屑病皮损形成过程中起重要的作用。参6(惠海英)20063144卡介菌多糖核酸对寻常型银屑病患者外周血单一核细胞产生干扰素-γ和白细胞介素-4的影响/李永喜(…     

一株曲霉果糖转移酶的发酵条件及转果糖基活性

对一株曲霉果糖转移酶菌株的产酶培养条件进行了研究。确定了最佳培养基组成：初始蔗糖质量浓度15～18g／dL，氮源为酵母膏，K2HPO4对果糖转移酶的产生具有明显的促进作用，添加0．2g／dLCMC能够使果糖转移酶活力提高到原来的1．3倍，在pH5．5，30℃条件下，果糖转移酶最高酶活力为30．42U／mL。HPLC分析结果表明，转糖基产物为总质量的55．8％。     

卡介菌多糖核酸联合派特灵液治疗复发性尖锐湿疣38例临床观察

尖锐湿疣是常见的性传播疾病之一,由人乳头瘤病毒感染引起,其发病率逐年上升是传染性很强的性传播疾病,传染途径主要是性接触传染.约70%以上通过不洁性交传染,少数通过间接接触传染.好发于外生殖器、会阴、肛门等部位.尖锐湿疣可以通过多种方法治疗,但易复发.我们用卡介菌多糖核酸(商品名:斯奇康)联合派特灵液治疗尖锐湿疣38例,无1例复发,均获满意效果.     

一期完成男转女变性手术1例

患者 ,男性 ,2 6岁。自信为女性 2 0年 ,有男转女变性要求8年于 2 0 0 1年 7月 2日入院。患者自 5～ 6岁开始自信自身为女性 ,喜爱女性装扮 ,行为女性化 ,不满自身男性生理特征及男性器官。曾有两次自残切除阴茎睾丸倾向 ,并自行服用雌激素 (避孕药 ) 4个月以改变男身 ,强烈要求做手术变性。经我院精神神经科检查除外其他精神病疾患 ,确诊为“易性病”,经心理治疗无效 ,病人对变性手术的后果有正确的认识 ,具有手术适应证。在签署办理好各有关书面材料并做好术前准备后 ,于 7月 1 9日在硬膜外麻下一次性完成男转女变性手术 ,同期完成阴茎、…     

植入式心律转复除颤患者的护理及随访

目的总结植入式心律转复除颤器(ICD)安置术的护理。方法2001年7月~2004年6月给予13例ICD患者进行了护理及随访。结果术后有2例患者出现心律失常“风暴”,经处理后未再出现心室颤动。随访患者1~4年,均存活,未发生晕厥等并发症。结论术前重视患者的心理护理,术后密切观察病情,积极预防心律失常“风暴”,并做好ICD健康教育,是ICD手术成功的重要护理措施。     

国产支架经纤维支气管镜介入肺减容术初步应用

纤维支气管镜肺减容术（bronchoscopic lung volume reduction，BLVR）因其微创、方法简单．近年来发展迅速.目前国外报道处于多中心试验阶段。笔者应用纤维立气管镜介入肺减容术（bronchoseopic interventional lung volume reduction BILVR）．在动物实验成功并获得医学伦理委员会批准后应用国产单向活瓣支架（专利申请号：200520078088.5，200520078089．X．图1）于2006年5月17日进行了1例临床试验．报告如下。