从传统走向现代——当代人才德育培养模式探析

从青年道德的现状出发 对当代人才德育培养模式提出几点思考。     

地塞米松对布比卡因诱导小鼠神经元毒性的影响

Objective To investigate the effect of dexamethasone on the toxicity of bupivacaine in murine neurons.Methods Murine neuroblastoma cell line N2a was obtained from ATCC cell bank (USA). The cells were cultured in 10% fetal cow serum/MEM culture medium and divided into 4 groups voup I control (Con); group II bupivacaine ( Bup); group Ⅲ dexamethasone (Dex) and group IV Dex + Bup. The culture medium contained bupivacaine 900 μmol/L in group Bup and dexamethasone 1 μmol/L in group Dex respectively. In group Dex + Bup ( IV ) Bup was added to the culture medium with a final concentration of 900 μmol/L at 12 h after pretreatment with Dex 1 μmol/L. The cells were inoculated in 24 well plates (0.5 ml in each well, 24 wells in each group) and 10 cm culture dishes (7 ml in each dish, 4 dishes in each group). The release rate of LDH was calculated and the morphology of the cells and nucleus condensation (by Hoechst 3334224 fluorescent staining) was detected at 9 h of incubation in 24 well plates. The mitochondrial transmembrane potential (by JC-1 assay) and phosphorylation of Akt and ERKs (by Western blot) were measured at 5 h of incubation in 24 well plates and in culture dishes respectively. ResultsBupivacaine caused severe damage to the N2a cells as evidenced by increase in LDH release and nucleus condensation (apoptosis), dephosphorylation of Akt and ERKs, decrease in mitochondrial transmembrane potential and severe morphological changes. Dexamethasone pretreatment significantly attenuated bupivacaine-induced neurotoxicity. Conclusion Dexamethasone can protect N2a cells from bupivacaine-induced neurotoxicity through stabilization of mitochondrial transmembrane potential and inhibition of dephosphorylation of Akt and ERKs.     

IN VITRO ANALYSIS OF τ PHOSPHORYLATION SITES AND ITS BIOLOGICAL ACTIVITY

INTRODUCTIONThemicrotubule－associatedprotein蚲ishyperphos－phorylatedandglycosylatedinAlzheimerdisease（AD），andtheseabnormalmodificationsformedthebasisofprogressivelyretrogradeneurofibrillarydegenerationseeninADbrainandtherebythedementia（１，２）．ADab－normallyphosphorylated蚲notonlyismicrotubuleas－semblyincompetent，butalsoinhibitsassemblyanddis－assemblesthepreassembledmicrotubulesinvitro（３）．Inthetangle－bearingneuronsinADbrain，thenormalcytoskeletonisdisruptedandreplacedwi…     

试论护生系统思维方式的培养

现代护理模式的建立 ,人们已不满足对局部的单因素分析 ,更注重对人体整体系统作出综合性多因素考察 ,这将促使护理工作者必须运用系统思维方式 ,来思考和处理护理学中的复杂问题。1　系统思维方式及其特征系统思维方式是将所要研究的对象 ,作为一个系统着重从系统的整体与要求、要素与要素、系统与环境之间的相互作用、相互联系的关系中综合地考察对象 ,以达到全面、精确地了解对象 ,科学准确地分析、判断 ,并对问题作最佳处理的思维方式。系统思维方式的特征 :整体性、综合性是系统思维方式的基本特征 ,具体体现在整体与部分之间、部分与…     

前列安栓联合体外射频热疗治疗前列腺痛

前列腺痛是成年男性的一种常见病 ,目前尚无特效的治疗方法 ,是泌尿外科临床急待解决的难题之一。我们自 2 0 0 1年 8月始应用前列安栓联合ＨＧ 2 0 0 0Ｐ型体外射频热疗仪治疗前列腺痛 6 3例 ,取得了满意疗效 ,现报告如下。1　资料与方法1 .1　临床资料本组 6 3例 ,年龄 2 6～     

创造性思维与医学研究生培养

本文阐述了创造性思维在医学研究生培养与成才过程中的作用,列举了目前研究生从入学到毕业后去向整个过程中,创造性思维的缺乏和院方培养力度的不足,提出了四点改革建议.     

输卵管积水造口术对体外受精与胚胎移植的影响

【目的】探讨在体外受精与胚胎移植 (IVF ET)之前输卵管积水患者行输卵管造口术对IVF ET治疗效果的影响。【方法】回顾分析 1999年 2月至 2 0 0 1年 1月因女性输卵管因素不孕行IVF ET治疗的 90 8个周期的资料。按输卵管积水患者在IVF ET前是否治疗分 3组 ,A组 :输卵管积水未手术治疗行IVF ET 2 3个周期 ,B组 :在IVF ET之前行输卵管积水造口术 (腹腔镜下或开腹 ) 2 2个周期 ,C组 :对照组 (输卵管阻塞 ,未发现输卵管积水 ) 86 3个周期。【结果】A组、B组、C组的IVF ET的种植率分别为 9 7%、17 9%、16 7% ,临床妊娠率分别为 2 1 7%、4 0 9%、39 2 %。A组的种植率及临床妊娠率比其它组低 ,经 χ2 检验 ,有统计学意义。【结论】输卵管积水未治疗行IVF ET的种植率及临床妊娠率较低 ,但在IVF ET之前行输卵管造口术可改善IVF ET的种植率及临床妊娠率     

中药“口康”含漱液体外抑菌试验和毒性试验研究

OBJECTIVE: To study the antibacterial activity of Chinese medicine kou-Kang mouthwash and its acute toxicity, and provide the basis of experiment and therapy for infectious diseases of the oral cavity. METHODS: Minimal inhibitory concentrations (MIC) of kou-Kang mouthwash were determined by the agar dilution method, and the acute toxicity was done. RESULTS: The range of MIC kou-Kang mouthwash against 27 Gram negative bacterial were 0.03-1.0 g.ml-1. MIC50 was 0.125 g.ml-1 and MIC90 was 0.125-0.5 g.ml-1. The range of MIC kou-Kang mouthwash against 21 Gram positive bacterial was 0.008-0.5 g.ml-1. The range of MIC50 and MIC90 were 0.015-0.125 g.ml-1 and 0.015-0.5 g.ml-1, respectively. The value of MIC was 0.015 g.ml-1 against candidia albicans. The acute toxic test indicated that the maximum tolerance dose was 417.6 g.kg-1 and 210 times as much as the clinical dose a day. No death of animals or toxicity and side-effect occurred in the test. CONCLUSION: Kou-Kang mouthwash shows a potent antibacterial activity against various pathogens of the oral cavity. Its antibiotic activity against Gram positive bacteria was 2-8 times stronger than that against Gram negative bacteria. It has low toxicity and can be used in clinical application.     

加强临床思维能力培养在传染病教改中的探索

人类社会已经跨入了 2 1世纪 ,依照世界医学发展的趋势和医学五年制本科专业学生的培养目标 ,根据国际、国内新传染病不断出现 ,老传染病死灰复燃的严峻形势 ,对人才培养也有更高更新的要求 ,进一步探讨提高学生临床分析和思维能力的教学改革模式迫在眉睫。 2 0 0 0～ 2 0 0 1年度教学中我们开展了一次备有尸体解剖结果的、难度较大 ,具有学科交叉性的临床病例讨论读书报告会 ,以培养学生临床思维能力。现报告如下 :1　材料在 97级 5年制统招生学生进入传染病临床桥梁课过程中 ,举办具有特色的读书报告会。此病例即 :因间发腹痛 10余年 ,发…