葡萄糖转运蛋白1和波形蛋白在肺癌胸水中的应用价值

目的:采用免疫细胞化学技术测定葡萄糖转运蛋白1(GLUT1)及波形蛋白(VM)在癌细胞中的定位表达,探讨GLUT1和VM在肺癌胸水中的应用价值。方法:以肺癌患者为研究对象,以肺癌性胸水为检测基础,收集胸水沉淀细胞,制成石蜡切块,采用免疫组化技术-链霉菌抗生物素-过氧化物霉法检测30例肺癌胸水和20例良性胸水中GLUT1和VM蛋白表达,统计单个及联合应用价值。结果:30例肺癌胸水中,GLUT1表达阳性的有25例(86.7%),良性胸水中间皮细胞VM表达阳性的有18例(90.0%),两种不同性质的胸水GLUT1和VM的阳性率表达均有差异(P<0.05),GLUT1和VM在肺癌胸水中表达呈负相关(P<0.05),两者联合诊断灵敏度为90%。结论:应用免疫组化方法检测肺癌胸水中GLUT1和VM蛋白表达,可提高肺癌早期诊断的灵敏度。     

iRoot SP��AH Plus�������ڸ�������Ч����Meta����

??Objective    To systematically evaluate the effect of iRoot SP and AH Plus paste in the treatment of root canal. Methods    Online databases including PubMed??EMBASE??the Cochrane Library??Web of Knowledge??and Chinese databases ??Wanfang database??VIP and CNKI?? were searched systematically up to February 2017. Randomized controlled trials and high-quality comparative studies were considered eligible for inclusion. Two qualified reviewers reviewed the original articles??evaluating qualities of articles??and extracting data independently. After heterogeneity test??Meta-analysis was performed. Results    In total??seven original clinical controlled trials were included??containing 493 teeth. iRoot SP paste could improve the immediate root canal quality ??OR=0.30??95%CI????0.10 ~ 0.85?????reduce the rate of postoperative pain ??OR=0.23??95%CI????0.06 ~ 0.89?????and decrease the apical leakage ??WMD=-0.13??95%CI????-0.22 ~ -0.05????. Conclusion    iRoot SP paste in root canal therapy can significantly alleviate pain??and the long-term curative effect is good??has better biological compatibility??which is an ideal root canal filling material. Because of the small number of the literatures included??which are all in China??it is necessary to include large sample??multicenter??randomized??double-blind??placebo-controlled trails to further confirm the clinical effect.     

Piperlongumine induces apoptotic and autophagic death of the primary myeloid leukemia cells from patients via activation of ROS-p38/JNK pathways

Aim:

To investigate the effects of piperlongumine (PL), an anticancer alkaloid from long pepper plants, on the primary myeloid leukemia cells from patients and the mechanisms of action.

Methods:

Human BM samples were obtained from 9 patients with acute or chronic myeloid leukemias and 2 patients with myelodysplastic syndrome (MDS). Bone marrow mononuclear cells (BMMNCs) were isolated and cultured. Cell viability was determined using MTT assay, and apoptosis was examined with PI staining or flow cytometry. ROS levels in the cells were determined using DCFH-DA staining and flow cytometry. Expression of apoptotic and autophagic signaling proteins was analyzed using Western blotting.

Results:

PL inhibited the viability of BMMNCs from the patients with myeloid leukemias (with IC₅₀ less than 20 μmol/L), but not that of BMMNCs from a patient with MDS. Furthermore, PL (10 and 20 μmol/L) induced apoptosis of BMMNCs from the patients with myeloid leukemias in a dose-dependent manner. PL markedly increased ROS levels in BMMNCs from the patients with myeloid leukemias, whereas pretreatment with the antioxidant N-acetyl-L-cysteine abolished PL-induced ROS accumulation and effectively reduced PL-induced cytotoxicity. Moreover, PL markedly increased the expression of the apoptotic proteins (Bax, Bcl-2 and caspase-3) and autophagic proteins (Beclin-1 and LC3B), and phosphorylation of p38 and JNK in BMMNCs from the patients with myeloid leukemias, whereas pretreatment with the specific p38 inhibitor SB203580 or the specific JNK inhibitor SP600125 partially reversed PL-induced ROS production, apoptotic/autophagic signaling activation and cytotoxicity.

Conclusion:

Piperlongumine induces apoptotic and autophagic death of the primary myeloid leukemia cells from patients via activation of ROS-p38/JNK pathways.     

Local cutaneous nerve terminal and mast cell responses to manual acupuncture in acupoint LI4 area of the rats

Previous studies have shown that the effects of manual acupuncture (MA) are contributed by collagen fibers and mast cells in local acupoints, at which acupuncture stimulation causes various afferent fiber groups to be excited. However what happens in local nerve fibers and mast cells after MA remains unclear. The aim of this study was to examine the response of cutaneous nerve fibers and mast cells to MA stimulation in acupoint Hegu (LI4). The contralateral LI4 of the same rat was used as a non-stimulated control. Immnohistochemistry analysis were carried out to observe the expression of histamine (HA), serotonin (5-HT) and nociceptive neuropeptides, calcitonin gene-related peptide (CGRP) and substance P (SP), in the LI4 area. Mast cells were labeled with anti-mast cell tryptase antibody and simultaneously with HA or 5-HT primary antibodies to observe their co-expression. Our results showed that SP and CGRP were expressed more highly on the cutaneous nerve fibers of LI4 after MA stimulation than that of the control. Mast cells aggregated in close proximity to the blood vessels in intra-epidermis and dermis and some of them with degranulation in the lower dermis and subcutaneous tissue of LI4. Both mast cells and their granules appeared with HA (+) and 5-HT (+) expression at stimulated L14 sites, while a few intact mast cells with a little expression of 5-HT and HA were distributed in areas of non-stimulated L14. The results indicated that local cutaneous nerve terminals and mast cells responded to MA with higher expression of SP and CGRP in nerve fibers, as well as with aggregation and degranulation of mast cells with HA and 5-HT granules at acupoint LI4. These neuroactive substances may convey signals to certain pathways that contribute to the effects of acupuncture.     

神门、三阴交穴位配伍改善失眠症患者焦虑、抑郁状态：随机对照研究

目的:观察电针神门、三阴交对失眠症患者焦虑、抑郁状态的影响,并探索其配伍效应的作用机制。方法:将90例失眠症患者随机分为配伍组、神门组和三阴交组,每组30例,并进一步筛选出37例焦虑状态患者(配伍组12例、神门组13例、三阴交组12例)和42例抑郁状态患者(配伍组14例、神门组14例、三阴交组14例)。配伍组、神门组、三阴交组分别予电针神门-三阴交、神门单穴及三阴交单穴,疏密波,频率5 Hz/25 Hz,治疗每次30 min,每天1次,连续治疗5 d为一疗程,共治疗2个疗程。分别于治疗前后评定各组患者失眠严重程度指数(ISI)、焦虑自评量表(SAS)和抑郁自评量表(SDS)评分,检测血清多巴胺(DA)和去甲肾上腺素(NE)含量。结果:与治疗前比较,治疗后3组患者ISI、SAS、SDS评分均降低(P<0.05),配伍组ISI评分低于神门组(P<0.05)。治疗后,3组失眠症焦虑状态患者ISI、SAS评分及血清DA含量均较治疗前下降(P<0.05),配伍组、神门组患者血清NE较治疗前下降(P<0.05);配伍组、神门组患者SAS评分、血清NE含量及配伍组ISI评分低...