脑血辨证之脑病证候与头风病证候对比分析

目的研究脑血辨证之脑病证候对头风病证候的适用性。方法采用统一的诊断标准，对每一入选病例根据脑病证候诊断标准和头风病证候诊断标准进行双重证候判别，并作配对分证，将配对分证结果列成双向无序R×C表，应用拟合优度疋。统计量进行独立性检验；应用KAPPA检验检验两种诊断标准的一致性，并计算证候符合率及灵敏度、特异度、假阴性率、假阳性率、似然比、符合率、正确诊断指数。结果两种辨证方法在证候分布方面互相独立，脑病证候与病情缓急、发病诱因、不良生活习惯、情志刺激、西医诊断病种具有明显的相关性。正确诊断指数为0．3731。Kappa值为0．3960。结论脑血辨证之脑病证候对头风病具有较好的适用性。脑病证候的分类建立于新病因理论的基础上，更加符合现代头风病病因病机的特点，具有较大的理论意义和实践价值，有利于头风病的辨证治疗。并且，脑血辨证规范化强，辨证思路清晰，易于掌握，错诊率低。     

HPLC法测定白花蛇舌草中槲皮素-3-O-β-d-吡喃葡萄糖苷的含量

目的:建立测定白花蛇舌草中槲皮素-3-O-β-d-吡喃葡萄糖苷含量的方法。方法:采用高效液相色谱法。色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),流动相为乙腈-1%冰醋酸(60∶40,V/V),检测波长为254 nm,流速为1.0 ml/min,柱温为35℃。结果:槲皮素-3-O-β-d-吡喃葡萄糖苷的质量浓度在5.24⁵2.40μg/ml范围内与其峰面积积分值呈良好线性关系(r=0.999 9);精密度、稳定性、重复性试验的RSD均<1.97%;平均加样回收率为99.57%,RSD=1.28%(n=6)。结论:该方法操作简便,结果准确、可靠,可用于白花蛇舌草药材中黄酮类活性成分的含量测定。     

中西医结合——中国医学发展的必由之路

对中西医结合的渊源和现状、定义和内涵、为什么要中西医结合、能否结合、如何结合,中西医结合的科学使命、主要障碍等问题进行了深入的思考,提出了个人见解。     

基于线粒体功能揭示《黄帝内经》“七七”理论内涵及高龄IVF-ET患者的中药干预机制

目的 研究不同年龄阶段女性卵巢颗粒细胞线粒体功能的变化及补肾中药二至天癸方对高龄妇女体外受精-胚胎移植技术（IVF-ET）结局的影响，从而验证《黄帝内经》“七七”理论的科学内涵。方法 收集山东中医药大学附属医院中西医结合生殖与遗传中心行辅助生殖技术的不孕症患者150例，按照《黄帝内经》“七七”理论分为三七、四七组（30例），五七组（60例）和六七组（60例），五七组和六七组采用随机数字+信封法分为对照组和中药组各30例，中药组自促排卵周期启动日给予二至天癸方至扳机日。观察各组之间的IVF结局，使用蛋白免疫印迹法（Western blot）测定各组卵巢颗粒细胞中线粒体融合蛋白1（Mfn1）、线粒体融合蛋白2（Mfn2），线粒体分裂蛋白1（Drp1）的含量。结果 与三七、四七组比较，五七对照组和六七对照组患者获卵数、双原核（2PN）数、可用胚胎数、优质胚胎数、临床妊娠率和活产率均明显下降（P<0.05），六七对照组鲜胚移植率明显下降（P<0.05）。与五七对照组比较，六七对照组患者患者获卵数、2PN数、可用胚胎数、优质胚胎数和临床妊娠率均明显下降（P<0.05）。与五七对照组比较，五七中药组患者获卵数、2PN数、可用胚胎数明显升高（P<0.05）；与六七对照组比较，六七中药组患者获卵数明显升高（P<0.05）。与三七、四七组比较，五七和六七对照组患者卵巢颗粒细胞中Mfn1和Mfn2蛋白表达明显降低，Drp1蛋白表达升高（P<0.05）；与五七对照组比较，六七对照组患者卵巢颗粒细胞中Mfn1和Mfn2蛋白表达明显降低，Drp1蛋白表达升高（P<0.05）。与五七对照组比较，五七中药组患者卵巢颗粒细胞中Mfn2蛋白表达升高，Drp1蛋白表达降低（P<0.05）；与六七对照组比较，六七中药组患者卵巢颗粒细胞中Mfn1和Mfn2蛋白表达升高，Drp1蛋白表达降低（P<0.05）。结论 随着年龄的增长，女性在“五七”后IVF结局预后变差，卵巢颗粒细胞中线粒体融合能力下降，而裂变增多，从线粒体功能验证了《黄帝内经》“七七”理论的内涵。二至天癸方可以调节高龄女性的卵巢颗粒细胞线粒体功能，提高Mfn1、Mfn2表达水平，促进线粒体融合，抑制Drp1而减少线粒体分裂，缓解年龄带来的卵巢功能减退，提高卵母细胞发育潜能，改善高龄女性的IVF结局，但对于“六七”之年的超高龄女性临床效果欠佳。     

Jinbei Oral Liquid ameliorates bleomycin-induced idiopathic pulmonary fibrosis in rats via reversion of Th1/Th2 shift

ObjectiveIdiopathic pulmonary fibrosis (IPF) is a progressive and lethal interstitial lung disease with high mortality. The pivotal role of Th1/Th2 immunological balance in the development and progression of IPF has been demonstrated previously. This study aimed to evaluate the effect of Jinbei Oral Liquid (JBOL) on IPF and its relationship with Th1/Th2 shift.MethodsRats were divided into six groups: control group, model group (bleomycin), pirfenidone group (positive group, 54 mg/kg, i.g.) and JBOL (5.4, 10.8 and 21.6 mL/kg, i.g.) groups. The rat model was established by an intratracheal instillation of bleomycin (BLM, 5 mg/kg). One day after injection of BLM, pirfenidone or JBOL was given to rats once daily within 28 consecutive days, respectively. Positron emission tomography/computed tomography (PET/CT) was performed on the treated rats. The extent of alveolitis and fibrosis was observed by H&E and Masson trichrome staining. The contents of TGF-β1, TNF-α, IL-4 and IFN-γ were further quantified by ELISA assay.ResultsPET/CT and histopathological evidence showed the ability of JBOL to attenuate bleomycin-induced alveolitis and fibrosis extent, and the alveolitis lesion score was markedly decreased compared with the model group. The increased expression of inflammatory cytokines TGF-β1 and TNF-α induced by bleomycin was also suppressed by JBOL. The Th1 response was limited by the reduced IFN-γ after BLM administration, and the Th2 response predominated significantly marked by the increased IL-4. JBOL could increase the level of IFN-γ and markedly increased the ratio of IFN-γ/IL-4.ConclusionThese findings suggested that JBOL may attenuate BLM-induced idiopathic pulmonary fibrosis via reducing inflammatory cell infiltration, pro-inflammatory cytokine release and excessive collagen deposition in rats. One of the mechanisms is the reversion of Th1/Th2 shift caused by BLM.