TLR4受体在肝癌细胞株HepG-2的表达

目的：探讨TLR4受体在肝癌细胞株HepG-2和肝癌组织中的表达。方法：用RT-PCR和Western-blot方法检测肝癌细胞株HepG-2中TLR4基因的转录和蛋白的表达的情况,同时用免疫组化的方法检测肝癌组织中TLR4的表达的情况。结果：肝癌细胞株HepG-2中存在TLR4基因的转录和蛋白的表达。TLR4受体主要表达位于肝癌组织细胞的细胞膜上,其阳性率约为76.7%。结论：肝癌细胞株HepG-2和肝癌组织中均有TLR4的表达。     

Pathological features of gastric cancer in Zhuanghe high-risk area in China during 1992–2005

Objective  To investigate the pathological features and chronological changes of 1003 cases with gastric cancer in Zhuanghe high-risk area during 1992–2005 and the relationship between the changes and etiology factors in order to make a clue for gastric cancer prevention. Methods  A total of 1003 gastric cancer specimens resected surgically between 1992–2005 in Zhuanghe Center Hospital were studied. The specimens were fixed in formalin and diagnosed by routine pathology. Results  The incidence of patients with gastric cancer was highest at age of 60–69, the next high was at age of 50–59 and it was significantly higher in male than in female (P<0.001), the ratio was 3.0:1. During the past 14 years, there were 159 (15.9%) EGC, 195 (19.4%) moderate and 649 (64.7%) advanced gastric cancer detected. In macroscopical features, type III remained dominant in EGC, the next was mixed type in EGC. In advanced gastric cancer the Borrmann’s type III remained the dominant, the next was type II. For nodal metastasis, positive cases were decreasing and negative were increasing in EGC, moreover negative cases were higher than positive ones each year. There was no obvious trend in advanced cancer but positive cases were higher than negative ones each year. In histological features, papillary, moderately and poorly differentiated tubular adenocarcinoma remained downtrend, mucus adenocarcinoma and undifferentiation cancer remained uptrend year after year. The radio of intestinal to diffuse type decreased from 0.78 to 0.62 during the past 14 years. Conclusion  There were significant chorological trends of pathological characteristic of gastric cancer in Zhuanghe high-risk area during the past 14 years. This work was supported by the National “Tenth-Five” Key Technologies R&D Program of China (2004BA703B04-02)     

Effects of Various Factors on Proliferation Capacity and Cytotoxicity of Induced CIK of Patients with Tumor In Vitro

Objective： To evaluate effects of different factors on proliferation capacity and cytotoxicity of induced cytokine induced killer （CIK） cells in vitro, so as to provide experimental basis for cell therapy of CIK in tumor. Methods： Cytometry and MTT assay were used in detecting proliferation capacity and cytotoxicity of CIK. Results： The proliferation capacity and cytotoxicity of CIK in vitro were stronger in the group of low age and serum free medium than in the group of advanced age or serum medium. And the proliferation of CIK in vitro increased with time during a certain period of incubation. Furthermore, CIK had equal cytotoxicity to various tumor cells. Conclusion： Various factors might influence the proliferation capacity and cytotoxicity of CIK of tumor patients in vitro.     

ESTABLISHMENT OF A HUMAN PANCREATIC ADENOCARCINOMA CELL LINE （JF305） WITH p53 EXPRESSION

李昕，王宏，姜奕，王晓华，贾兰玲，张宝庚ＥＳＴＡＢＬＩＳＨＭＥＮＴＯＦＡＨＵＭＡＮＰＡＮＣＲＥＡＴＩＣＡＤＥＮＯＣＡＲＣＩＮＯＭＡＣＥＬＬＬＩＮＥ（ＪＦ３０５）ＷＩＴＨｐ５３ＥＸＰＲＥＳＳＩＯＮ￥ＬｉＸｉｎ；ＷａｎｇＨｏｎｇ；ＪｉａｎｇＹｉ；Ｗａｎｇ...     

EFFICACY OF IFOSFAMIDE AND VP-16 （ETOPOSIDE） IN PATIENTS WITH SMALL CELL LUNG CANCER AND THE CORRELATION BETWEEN MICROVESSEL COUNT ON CHEMOSENSITIVITY

Objective： To evaluate the efficacy of ifosfamide and etoposide （VP-16） in patients with small cell lung cancer （SCLC）, and investigate the correlation between microvessel count （MVC） in tumor and chemotherapeutic sensitivity. Methods： Forty-one consecutive cases of SCLC received chemotherapy of ifosfamide plus VP-16, and underwent investigation retrospectively. Immunohistochemistry using anti-human blood type H monoclonal antibody was conducted and MVC was recorded under light microscope. Results： There were 27 limited-disease and 14 extensive-disease patients. The overall response rate was 92.7% （38/41） with 28 cases （68.3%） of complete response （CR）, 10 （24.4%） with partial response （PR）, 3 （7.3%） with progressive disease （PD）. The 1-, 2-, 3-, and 5-year survival rates were 68.3% （28/41）, 48.3% （20/41）, 23.7% （9/38） and 11.1% （3/27）, respectively, with the median survival of 26.8 months. The principal toxicities were grade 3-4 neutropenia in 8 cases （19.5%）, grade 3-4 thrombocytopenia in 6 cases （14.6%）, mild liver toxicity in 7 cases （17.0%） and mild renal function damage in 4 cases （9.8%）. The mesenchymal vasculature was clearly visualized, with the mean value of 34.7 under each high microscopic power field. Of SCLC with more MVC （n=26）, CR accounted for 84.6%; while in cancers with less MVC （n=l 5）, CR took up 40.0%, with significant difference （P〈0.05）. Conclusion： Administrating ifosfamide and VP-16 is in accordance with the biological features of SCLC and results in beneficial results as well as acceptable side effects. The MVC is positively correlated with the chemotherapeutic sensitivity, and serves as a vital factor contributing to chemosensitivity.     

EXPRESSION OF β-CATENIN AND C-MYC IN THYMOMA AND THE ASSOCIATION WITH BIOLOGICAL FEATURES

Objective： β-catenin is closely associated with proliferation, differentiation, invasion and metastasis of tumor cells. C-myc is proved to be abnormally expressed in various kinds of malignant neoplasmas. This study is aimed to investigate the features of β-catenin and C-myc expressions in thymoma and the corresponding clinical significance. Method： Immunochemistry was used to detect the features of expressions of β-catenin and C-myc in thymoma and normal thymus. Of the 105 samples, according to Beratz classification, there were： epithelial type 34; lymphocyte type 35; mixed type 31 and shuttle cell type 5. According to Masaoka classification, there were： stage 1： 38; stage 2： 32; stage 3：20 and stage 4： 15. According to WHO classification, there were： type A 20; type AB 19; type B1 21; type B2 17; type B3 13 and type C 15. Results： Different ways of expression was revealed in different stages of thymomas. With the progress of thymoma, the expression of β-catenin on cell membrane decreased, while increased in cytoplasma and nucleus, even with nucleus shift. The expression of C-myc increased in invasive thymoma. Abnormal expression of β-catenin and C-myc increased, with significant difference（P〈0.05）. Conclusion： The different ways of expression and diversity of expression level may indicate the biological characters of thymoma, and could be used as an objective index for invasive thymoma.     

碳青酶烯类抗菌药物对常见非发酵革兰阴性杆菌体外抗菌活性的分析

目的比较美罗培南和亚胺培南对铜绿假单胞菌和鲍曼不动杆菌的体外抗菌活性，为临床医生经验用药提供参考。方法采用K-B纸片法和MIC法对2004-2006年分离的铜绿假单胞菌587株和鲍曼不动杆菌226株进行药敏试验．结果按照CLSI2006标准判定，数据采用WHONET5．4和SPSS11．5进行统计分析。结果铜绿假单胞菌对亚胺培南和美罗培南的耐药率分别为43．4％和37．5％，P〈0.01；鲍曼不动杆菌对亚胺培南和美罗培南的耐药率为40．7％和41．6％，P〉0.05。结论非发酵菌对碳青酶烯类抗菌药物耐药问题越来越严重，该研究发现美罗培南对铜绿假单胞菌的耐药率低于亚胺培南，对鲍曼不动杆菌耐药率无统计学意义。美罗培南和亚胺培南对铜绿假单胞菌的耐药性存在差异，应该加强对这两种抗菌药物的监测。     

构建面向应用的医学特色计算机课程教学体系

概述国内外医学信息技术与职业技能教育的发展趋势，以及信息时代对医学人才IT技术知识结构、技能结构的新要求。强调医学计算机基础教育应该紧密结合本专业医信技术应用的需求，紧跟本学科未来发展的方向，科学合理的完善大学生的IT知识结构，同时以“生态学”教育模式培养学生自主学习的意识及方法，使各层次医药类院校培养的学生能够适应各自专业工作中对网络技术、信息技术和本专业数字化技术的要求，为将来从事数字诊疗技术夯实基础。提出要用科学发展观深化教育改革，构建面向应用的医学特色计算机课程体系，又好又快地落实培养适应未来信息社会发展需求的现代医学人才目标。     

一氧化氮对腹膜间皮细胞醛糖还原酶活性的影响

目的通过观察一氧化氮对腹膜间皮细胞醛糖还原酶（AR）活性的影响，探讨一氧化氮对腹膜间皮细胞醛糖还原酶的调节作用。方法采用人的网膜作为细胞来源，胰蛋白酶-EDTA消化法进行人腹膜间皮细胞（HPMC）的分离、培养，间接免疫荧光法对第二代细胞进行鉴定。第2-3代细胞用于实验。以硝普纳为一氧化氮供体，硝普纳（0．5mmol／L、1mmol／L、2mmol／L）＋1．5％葡萄糖培养液为实验组，以无一氧化氮1．5％葡萄糖培养液为对照组，观察剂量及时间对腹膜间皮细胞醛糖还原酶活性的影响。应用紫外分光光度计记录NADPH在340nm处吸光值的下降表示醛糖还原酶活性。结果高糖状态下各浓度一氧化氮组醛糖还原酶活性均高于对照组，且醛糖还原酶活性随一氧化氮浓度的增加而增加；一氧化氮＋葡萄糖组醛糖还原酶活性随着时间的延长而逐渐增加。各时点一氧化氮＋葡萄糖组醛糖还原酶活性均高于葡萄糖组。结论一氧化氮以时间及剂量依赖的方式使腹膜间皮细胞醛糖还原酶活性增加。     

颈动脉粥样硬化及hs—CRP与急性脑梗塞的临床关系

目的探讨急性前循环脑梗塞患者的颈动脉粥样硬化程度，以及与血清高敏C反应蛋白（hs—CRP）的关系。方法对107例急性前循环脑梗塞病患者与95例正常对照者行颈动脉超声检查，观察指标包括颈动脉内-中膜厚度（IMT）颈动脉粥样斑块的位置及形态、大小和回声特点，并进行血清hs—CRP浓度的检测。结果研究发现颈动脉粥样硬化及hs—CRP与前循环脑梗塞具有显著相关性；hs—CRP浓度与颈动脉粥样硬化斑块性质有显著相关性，即不稳定斑块hs—CRP浓度高。结论颈动脉超声能显示动脉粥样斑块的相关物理特性，对脑梗塞的疗效观察与预测具有十分重要的意义；hs—CRP是反映机体炎症反应的敏感指标，与颈动脉粥样硬化、斑块性质及前循环脑梗塞有显著相关，可反映颈动脉粥样硬化严重程度、脑卒中的危险性及顸后。