Magnesium hydroxide temporarily enhancing osteoblast activity and decreasing the osteoclast number in peri-implant bone remodelling

Repeated observations of enhanced bone growth around various degradable magnesium alloys in vivo raise the question: what is the major mutual origin of this biological stimulus? Several possible origins, e.g. the metal surface properties, electrochemical interactions and biological effects of alloying elements, can be excluded by investigating the sole bone response to the purified major corrosion product of all magnesium alloys, magnesium hydroxide (Mg(OH)₂). Isostatically compressed cylinders of pure Mg(OH)₂ were implanted into rabbit femur condyles for 2–6 weeks. We observed a temporarily increased bone volume (BV/TV) in the vicinity of Mg(OH)₂ at 4 weeks that returned to a level that was equal to the control at 6 weeks. The osteoclast surface (OcS/BS) was significantly reduced during the first four weeks around the Mg(OH)₂ cylinder, while an increase in osteoid surface (OS/BS) was observed at the same time. At 6 weeks, the OcS/BS adjacent to the Mg(OH)₂ cylinder was back within the same range of the control. The mineral apposition rate (MAR) was extensively enhanced until 4 weeks in the Mg(OH)₂ group before matching the control. Thus, the enhanced bone formation and temporarily decreased bone resorption resulted in a higher bone mass around the slowly dissolving Mg(OH)₂ cylinder. These data support the hypothesis that the major corrosion product Mg(OH)₂ from any magnesium alloy is the major origin of the observed enhanced bone growth in vivo. Further studies have to evaluate if the enhanced bone growth is mainly due to the local magnesium ion concentration or the local alkalosis accompanying the Mg(OH)₂ dissolution.     

Preventive effects of fenofibrate on insulin resistance, hyperglycaemia, visceral fat accumulation in NIH mice induced by small-dose streptozotocin and lard.

High-fat diets and oxidative damage may contribute to the development of type 2 diabetes. Hypolipidaemic drugs and antioxidants were supposed to prevent the development of the disease. In this study, we investigated preventive effects of fenofibrate (200 mg kg(-1)), vitamin C (30 mg kg(-1)), combination of both in mice induced by streptozotocin (35 mg kg(-1)) and soluble lard (15 ml kg(-1)). The results showed the mice demonstrated hyperglycaemia and hypercholesterolaemia, visceral fat accumulation, and a slight increase in liver glycogen/triglyceride and oxidative stress within 60 days of treatment. Fenofibrate enhanced insulin sensitivity, improved glycaemic control, lowered serum triglycerides, reduced body and visceral fat weights, and decreased liver glycogen/lipid levels but showed hepatotoxicity in the mice. Vitamin C neither itself prevented nor enhanced preventive effects of fenofibrate on glucose and lipid metabolism but partly attenuated the hepatotoxicity of fenofibrate. These results suggest that fenofibrate inhibit development of type 2 diabetes induced by high-fat diets and oxidative stress. However, here, vitamin C just can serve as an adjunct to fenofibrate therapy against its hepatotoxicity. In the future study, we should investigate if higher dosage of vitamin C or other antioxidants would enhance preventive effects of fenofibrate in type 2 diabetes.     

4(3H)-喹唑啉酮衍生物的合成及体外抗肿瘤活性

将6-溴甲基-2-甲基-4(3H)-喹唑啉酮在无水磷酸钾存在下与二硫化碳以及不同的胺反应，合成了一系列具有二硫代氨基甲酸酯侧链的4(3H)-喹唑啉酮衍生物，其结构经ESI-MS，¹H NMR，元素分析或HRMS所证实。采用MTT法测定了目标化合物8a~8q对人慢性髓性白血病K562细胞和人宫颈癌Hela细胞的体外抗肿瘤活性，结果表明化合物8q对K562和Hela细胞的体外生长具有显著的抑制作用，IC₅₀值分别为0.5和12.0 μmol·L^-1，因而可作为抗肿瘤药物研究的先导化合物。     

医药代表专业素质评分及定级标准研究

目的:为医药企业在医药代表评分、定级管理方面提供有益参考。方法:从医药代表对所销售药品专业知识的掌握程度、一对一的医师拜访和小型科室会组织的能力等多角度展开细致的研究分析,建立医药代表专业素质评分及定级标准。结果与结论:所建立的医药代表专业素质评分及定级标准具有可操作性,相对客观完善。     

基于Internet的生理多参数远程实时监护系统设计

基于Internet设计一种生理多参数远程实时监护系统，该系统采用混合式P2P（Peer to Peer）网络技术进行生理参数的远程实时传输。实验结果表明，利用P2P能极大地提高网络数据传输速度，可实现心电信号等生理参数的远程实时监护。     

血清孕酮与β-HCG联合测定在异常妊娠早期诊断中的价值

目的探讨血清孕酮与人绒毛膜促性腺激素β亚单位(β-HCG)联合测定在异常妊娠早期诊断中的价值。方法选择异常妊娠患者232例,根据妊娠结局分为先兆流产组(149例)、稽留流产组(51例)和异位妊娠组(32例),监测各组血清孕酮与β-HCG。结果先兆流产组孕酮值,明显高于稽留流产组(P<0.01),高于异位妊娠组(P<0.05);先兆流产组β-HCG值明显高于稽留流产组和异位妊娠组(P<0.01)。结论血清孕酮与β-HCG联合测定可作为早期诊断异常妊娠的一种手段。     

高血压人群社区干预的效果评价

目的：评价高血压人群社区干预的效果。方法：对本辖区两个社区35岁以上人群进行健康普查。检出的627例高血压病人作为研究对象。在其中一个社区有组织、有计划地按照《深圳市高血压社区综合防治项目工作手册》进行健康教育和干预。结果：经过社区卫生服务平台综合干预后，患者危险因素知晓率明显升高（P〈0．01），生活方式、行为及防治的依从性显著升高（P〈0．01），心脑血管意外事件发生率降低（P〈0．05），患者用于高血压病药物治疗的年平均费用降低（P〈0．01）。结论：通过社区卫生服务平台综合干预能显著提高高血压病的防治效果，在社区中具有一定的推广价值。