白细胞介素-17对老年类风湿关节炎患者成纤维样滑膜细胞增殖和趋化因子分泌的影响及作用机制

目的探讨白细胞介素(IL)-17对老年类风湿关节炎(RA)患者成纤维样滑膜细胞增殖和趋化因子分泌的影响及作用机制。方法选取行关节置管术的老年RA患者,从术中切除的新鲜滑膜组织中分离滑膜成纤维细胞进行培养。3-(4,5-二甲噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法检测IL-17对细胞增殖的影响;蛋白芯片试剂盒检测滑膜成纤维细胞上清液趋化因子[中性粒细胞激活肽(ENA)-78、IL-8、生长相关癌基因(GRO)、单核细胞趋化蛋白(MCP)-1]的水平;流式细胞术检测滑膜成纤维细胞表面IL-17受体(IL-17R)的表达。在成纤维样滑膜细胞中分别加入MEK1/2信号通路抑制剂(PD98059)、核转录因子(NF)-κB信号通路抑制剂(LY294002)、STAT3信号通路抑制剂(AG490)、RANKL信号通路抑制剂(小白菊内酯),孵育60 min后加IL-17刺激72 h,荧光定量PCR法检测ENA-78、IL-8、GRO、MCP-1 mRNA表达水平。结果 IL-17对滑膜成纤维细胞各时间点刺激指数差异均有统计学意义(P<0.05),其中刺激72 h滑膜成纤维细胞增殖能力最强。IL-17刺激滑膜成纤维细胞48 h后,滑膜成纤维细胞分泌的ENA-78、IL-8、GRO、MCP-1水平均明显高于对照组(P<0.05)。流式细胞术显示,加入抗IL-17R抗体组IL-17R表达水平明显高于同型对照组(P<0.05)。滴加PD98059、LY294002、AG490后,ENA-78、IL-8、GRO、MCP-1 mRNA表达量均明显低于空白对照组(P<0.05);而滴加小白菊内酯后与空白对照组差异无统计学意义(P>0.05)。结论 IL-17可促进老年RA成纤维样滑膜细胞增殖及趋化因子表达;IL-17可能通过MEK1/2、NF-κB、STAT3信号通路诱导滑膜成纤维细胞趋化因子表达。     

可摘局部义齿对老年中重度牙周炎伴2型糖尿病患者牙周状况及全唾液中MMP-8水平的影响研究

目的    探讨戴用可摘局部义齿对老年中重度牙周炎伴2型糖尿病患者牙周状况及全唾液中基质金属蛋白酶（MMP）-8水平的影响。方法    选取2015年7—12月在承德医学院附属医院口腔科行远中游离端铸造支架式可摘局部义齿修复的老年中重度牙周炎伴2型糖尿病患者16例（试验组）和单纯老年中重度牙周炎患者14例（对照组）,分别于可摘局部义齿修复前及戴用义齿1、3个月后检测两组患者基牙临床牙周指标和全唾液中MMP-8含量。 结果    两组患者戴用义齿1、3个月后的各项牙周指标值及全唾液中MMP-8水平均明显高于义齿修复前（P < 0.05）;与戴用义齿1个月后相比较,戴用义齿3个月后牙龈指数（GI）及全唾液中MMP-8水平显著降低（P < 0 .05）;戴用义齿1、3个月两次复查时,两组患者颊舌侧的菌斑指数（PLI）存在显著性差异（P < 0.05）,舌侧PLI明显高于颊侧。两组间比较,患者在修复前、戴用义齿1个月后的各牙周指标值相似,差异无统计学意义（P > 0.05）;戴用义齿3个月后,两组患者的基牙PLI值差异不明显,而基牙GI及全唾液中MMP-8水平的差异有统计学意义（P < 0.05）,试验组明显高于对照组。结论    老年中重度牙周炎伴2型糖尿病患者戴用可摘局部义齿后牙龈炎症较重;戴用可摘局部义齿可引起老年中重度牙周炎伴2型糖尿病患者全唾液中MMP-8表达水平升高。     

初级卫生保健与学生心理卫生服务(三)

以初级卫生保健为基础的社区学生心理卫生服务体系,其最基层、最基本的单位是学校。根本任务是：以努力开展学生心理健康教育为突破口,改善学生的心理素质和人格品质,增强心理健康意识,逐步改变心理行为,提高自我保健能力,适应现代生活,高效能地完成学习任务,使卫生工作更好地为教育服务。人的心理健康问题,是在成长过程中因受到各种不良因素影响而逐渐积累和发展起来的。我国正处在进一步深化改革、扩大开放的新时期。社会环境的变迁,思想观念的转变,社会文化的演变,各种信息的激增,社会竞争的加剧,人际关系的复杂化,增加了…     

Mechanism of Buyang Huanwu Decoction alleviating inflammatory response after cerebral ischemia/reperfusion injury by activating SIRT1 in hippocampus of rats北大核心CSCD

Aim To explore the inhibitory effect of Buyang Huanwu Decoction on the inflammatory response in the hippocampus of brain tissues of CIRI rats by regulating SIRT1 and the underlying mechanism. Methods The middle cerebral artery embolization (MCAO) model was prepared in rats and divided into sham operation group (Sham), model group (MCAO/R), Buyang Huanwu Decoction group (BYHWT),and BYHWT + SIRT1 inhibitor group (BYHWT + EX527). Zea Longa was used to detect the neurological function score of rats in each group; TTC staining was used to determine the volume of cerebral infarction; HE staining was used to observe the pathological damage of the hippocampus; Western blot was used to detect the expression levels of SIRT1 and IL-6; immunohistochemistry was used to detect TNF-α, IL-1β expression level. Results Compared with the sham group,the neurological function score of the MCAO/R group increased (P < 0.05); the volume of cerebral infarction increased (P < 0.05); the nerve cells in hippocampus were severely damaged, arranged disorderly, and the nucleus was broken; Western blot showed that the expression of SIRT1 decreased, IL-6 expression increased (P <0.05); immunohistochemistry showed that TNF-α,IL-1β expression increased (P < 0.05). Compared with the MCAO/R group, the neurological function score of the BYHWT group decreased (P <0.05); the volume of cerebral infarction decreased (P < 0.05); the damage of nerve cells in hippocampus was reduced; Western blot showed that the expression of SIRT1 increased and IL-6 expression decreased (P < 0.05); immunohistochemistry showed that TNF-α, IL-1β expression decreased (P < 0.05). Compared with the BYHWT group, the neurological function score of the BYHWT + EX527 group increased (P < 0.05); the volume of cerebral infarction was raised (P <0.05); the damage of nerve cells in hippocampus was aggravated; Western blot showed that the expression of SIRT1 decreased and IL-6 expression increased (P < 0.05); immunohistochemistry showed that TNF-α, IL-1β expression increased (P < 0.05). Conclusions Preliminary discussion of Buyang Huanwu Decoction can activate SIRT1 in hippocampus of rat brain tissues to reduce the inflammatory response after CIRI and play a role in brain protection. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Nrf2基因启动子多态性与妊娠期糖尿病合并生殖道感染PROM易感性的关联

目的 探究核因子E2相关因子2(Nrf2)基因启动子多态性与妊娠期糖尿病(GDM)合并生殖道感染患者足月胎膜早破(PROM)易感性的关联。方法 选取2017年1月-2020年6月在承德医学院附属医院定期产检、分娩的GDM产妇175例，分析产妇妊娠期生殖道感染情况和感染病原菌的病原学特点；根据产妇是否发生PROM分为PROM组46例和非PROM组129例，另选取同期于医院正常分娩的健康产妇105名为对照组，聚合酶链反应(PCR)检测Nrf2基因启动子-653G/A、-651G/A和-617C/A位点的多态性。结果 57例(32.57%)GDM产妇发生生殖道感染，检出的89株感染病原菌以革兰阳性菌为主(29.21%)。PROM组、非PROM组和对照组的生殖道感染率比较：PROM组>非PROM组>对照组(P<0.05),新生儿出生体质量比较：PROM组<非PROM组<对照组(P<0.05)。PROM组Nrf2基因启动子-617C/A位点的CC基因型、C等位基因频率均低于非PROM组和对照组(P<0.05)。Logistic回归分析结果显示，生殖道感染...