盐酸美金刚胶囊人体药代动力学研究

目的：进行健康志愿者盐酸美金刚胶囊单次和多次口服的药代动力学研究。方法：采用高效液相色谱串联质谱法测定美金刚血浓度,以DAS2.0计算其药代动力学参数。结果：盐酸美金刚胶囊5、10和15 mg单次口服,美金刚药代动力学参数tmax为（5.6±1.2）、（5.2±2.0）和（4.9±1.1）h;Cmax为（4.4±0.9）、（8.9±1.6）和（16.5±2.8）ng/mL;t1/2为（47±10）、（47±9）和（51±9）h。10 mg多次口服,美金刚药代动力学参数tmax为（6.0±1.4）h,Cmax为（31.8±6.0）ng/mL,t1/2为（46±8）h。结论：美金刚单次给药,Cmax和AUC有良好剂量相关性;多次给药可发生蓄积。     

Treatment of Myelodysplastic Syndrome with Cyclosporin A

A multicenter prospective study was carried out to evaluate the efficacy of cyclosporin A (CsA) in 32 patients with myelodysplastic syndromes. The FAB subtypes of the patients included refractory anemia, refractory anemia with ringed sideroblasts, and refractory anemia with excess blasts. The dosage of CsA was 3 to 6 mg/kg per day and was adjusted according to the blood concentration of CsA and the responses of patients. The drug was administered twice a day for more than 3 months. After 3 months of treatment, hematological improvement was observed in 18 of 32 patients (56.3%) by the criteria of the International Working Group. At the end of the follow-up (median time, 14 months), 4 patients showed alteration of disease progression, including 1 complete remission and 3 partial remissions, and 16 patients showed hematological improvement. There were a total of 20 responders. The response rate was 62.5% (20/32). It was shown that the CsA therapy was effective in patients with refractory anemia or refractory anemia with excess blasts and both hypo- and hyperplastic bone marrows might respond to the therapy. The survival time was significantly longer in responders than in nonresponders. The study shows that CsA therapy is potentially the most effective therapy for myelodysplastic syndromes.     

Repetitive transcranial magnetic stimulation does not influence immunological HL-60 cells and neuronal PC12 cells

BACKGROUND: Transcranial magnetic stimulation (TMS) is a non-invasive method used in medical applications such as brain mapping or as a therapeutic tool in neurological and psychiatric disorders because it can stimulate defined regions of the brain without anaesthesia. METHODS: The action of repetitive transcranial magnetic stimulation (rTMS) on HL-60 and PC12 cells has been investigated. The cells have been stimulated in vitro with different number of pulses (75 - 1250), different intensities (10, 20 and 40%) and different frequencies (0.25, 1 and 10 Hz) by using a double coil (2x70 mm) connected to the 'Magstim rapid'. At selected time points after treatment the following endpoints have been determined: viability, cyclic AMP (cAMP) and heat shock protein 72 (Hsp72) (HL-60 cells), and viability, cAMP, dopamine and noradrenaline (PC12 cells). Viability was measured with the alamarBlue assay, whereas cAMP, Hsp72, dopamine and noradrenaline were determined with enzyme-linked immunosorbent assay (ELISA). RESULTS: In both cell lines viability was not influenced by rTMS treatment, the same was true for the cytosolic cAMP concentration. In HL-60 cells rTMS treatment did not change the Hsp72 content, also a protective effect of rTMS treatment on cell viability before toxic H(2)O(2) treatment was not observed. After high potassium treatment the release of the two neurotransmitters dopamine and noradrenaline in PC12 cells was enhanced 15- and 5-fold, respectively, but after rTMS treatment no change in the release of the two neurotransmitters was observed. CONCLUSIONS: In two mammalian cell lines rTMS treatment in a variety of exposure conditions does not influence any of the measured parameters.     

火山蕨矿泉水煎液对外阴阴道假丝酵母菌病疗效观察的报告

目的:探讨火山蕨矿泉水煎液对外阴阴道假丝酵母菌病的疗效。方法:180例患者随机分为两组,对照组90例予制霉菌素(米克定)栓剂治疗,观察组90例给予火山蕨矿泉水煎液治疗。结果:对照组总有效率92.22%,复发率17.78%;观察组总有效率91.11%,复发率5.56%。两组总有效率仅差1.11%,比较无显著性差异,但观察组的治愈率和显效率均比对照组高,尤其是观察组复发率较低(P<0.05)。结论:火山蕨矿泉水煎液治疗外阴阴道假丝酵母菌病费用低、纯天然、无任何副作用,疗效显著。     

铜,锌对培养心肌细胞缺氧／再给氧损伤的影响

本文观察了不同浓度的铜、锌对培养乳鼠心肌细胞缺氧/再给氧损伤后超氧歧化酶(superoxid dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-px)活性和脂质过氧化(lipid peroxidation,LPO)代谢产物丙二醛(malondialodehyde,MDA)含量改变,以及乳酸脱氢酶(lactate dehydrogenase,LDH)释放的影响。结果表明:缺氧/再给氧后,心肌细胞SOD活性降低,GSH-px活性和MDA含量增高,LDH释放增加。铜、锌均能不同程度地提高SOD活性,但铜的作用强于锌。锌、铜均可降低LDH的释放,但锌的作用强于铜,锌、铜浓度过高反而增加LDH的释放。此外,铜使GSH-px活性明显升高,并显著降低MDA含量。     

神经衰弱患者的十年随访研究

目的　探讨神经衰弱患者的发展变化。方法　采用队列内病例对照研究 ,对 1992年诊断为神经衰弱患者十年后进行随访 ,完成随访 98例。结果　诊断改变为 4 1% ,造成诊断改变相关因素有精神病家族史、起病年龄、症状反复发作、伴躯体症状 ;8个精神病早期症状中 7个有显著性差异 (P <0 0 5 )。结论　神经衰弱患者随着时间的发展有较高的诊断改变率 ,伴有精神病早期症状的患者更易发展为精神病。     

慢性前列腺炎症状评分及病原学分析

目的：了解慢性前列腺炎（ＣＰ）患者的症状评分（ＮＩＨ—ＣＰＳＩ）及前列腺液（ＥＰＳ）的细菌感染情况。方法：对２６１例ＣＰ患者进行症状评分及对其ＥＰＳ标本进行常规培养、淋病奈瑟菌的特殊培养和支原体、衣原体测定。结果：９６例ＥＰＳ细菌培养阳性。其中单一Ｇ＾＋菌感染４９例．单一Ｇ＾－菌感染２７例，１１例为２种细菌感染，９例为３种细菌混合感染。在２６１例中，单独支原体感染４６例，衣原体感染２１例，支原体、衣原体合并感染１１例。症状评分高者，细菌培养阳性率高。结论：ＣＢＰ的致病菌种类较多，与症状评分成正相关。