成骨肉瘤相关抗原的纯化及其特异性分析

用抗正常组织抗体制成免疫亲和层析柱，把成骨肉瘤组织提取物反复过此免疫亲和层析柱，其中的正常组织成分被吸附在层析柱上，不被吸附的成分经ＳＤＳ－ＰＡＧＥ分析，显示单一条带，是一种分子量为３２ＫＤ的抗原成分。利用免疫电泳、ＥＬＩＳＡ和琼脂双向扩散试验进行了特异性分析，结果表明，此抗原只在肿瘤组织中存在，而在正常组织中没有。用此抗原免疫ＢＡＢＬ／Ｃ小鼠制备了一株抗成骨肉瘤的单克隆抗体２Ｇ１０。用此单抗进行免疫组织化学染色，与正常组织反应阳性率为０／９，与成骨肉瘤组织反应阳性率为２０／２１。     

应用射频迷宫术治疗心房纤颤

１９９４年１０月于１９９５年８月采用射频迷宫术先天性继发孔房缺合并心房纤颤１例，瓣膜替换术的治疗风湿性二尖瓣病变合并心房纤颤９例。术后房颤全部消失，呈窦性心律６例，结性心律３例，心房扩动１例。作者认为，此方法操作简单易行，效果确实，手术时间短，避免了心脏多处切口，渗血少，术后平顺，无近期并发症。     

加强学科建设推进医院全面发展

医院要发展，学科是基础，人才是关键，水平是标志。如何处理好这三者的关系，文章着重就把握优化学科结构的基本点、确立面向未来的基准点、占领学科前沿的制高点、抓好人才梯队的关键点、打牢物质条件的支撑点、强化医疗科研的结合点、选准学科投入的着力点等问题，进行了有益的探讨。     

A Dynamic and Quantitative Study of Pattern Visual Evoked Potentials and Gamma-Aminobutyric Acid Neurones in the Lateral Geniculate Nucleus and the Visual Cortex of Monocular Deprivation Cats

Purpose: To assess the effects of monocular lid closure during critical period on cortical activity.Method: Pattern visual evoked potentials (PVEP) of the normal and the monocular deprivation (MD) cats were dynamically measured and the number of gam-maaminobutyric acid immunopositive (GABA-IP) neurones of the area 17 of the visual cortex and the lateral geniculate nucleus (LGN) was quantitatively compared by using immunohistochemical method (ABC).Result: The amplitude of the N1-P1 attenuated in deprived eyes (DE) , NE/DE at postnatal week (PNW) 7-8 (P<0. 05), NE/DE at PNW 15-16 (P<0. 01); while P1 latency delayed, NE/DE at PNW 7-8 (P>0. 05), NE/DE at PNW 15 - 16(P<0. 05). The numbers of GABA-IP neurones in layer A1 of the ipsilateral LGN and in layer A of the contralateral LGN, compared to those in the corresponding normal laminae, were not significant at PNW 7 - 8 and PNW 11 - 12 (P >0. 05), while in the same cats a reduction in the number of GABA-IP neurcnes was found in layer IV of area 17 at PNW 11     

Percutaneous treatment of non-contained lumbar disc herniation by injection of oxygen–ozone combined with collagenase

PurposeTo evaluate the therapeutic results of oxygen–ozone combined collagenase injection for the treatment of lumbar disc herniation compared to the surgery. And to explore the role of this minimally invasive treatment as an alternative to disc surgery.Materials and methodsTwo groups of patients (n = 108) were treated with different ways respectively. Minimally invasive group of patients was treated with the injection of oxygen–ozone combined with collagenase into the lumbar disc or the epidural space; the other group was treated with traditional surgery. After the treatment, the patients were followed-up and the therapeutic effect was assessed at 2 weeks, 3 and 12 months by the modified Macnab criteria.ResultsThe success rate was 86.11% and 88.89% in minimally invasive group at 3 and 12 months respectively, while 92.59% and 95.37% in surgical group. There was no statistically significant difference between two groups at 3 and 12 months (P = 0.123, P = 0.08). However, the surgical group produced a statistically significant greater improvement for back pain and disability in the first few weeks (P = 0.0001). The success rate was 51.86% and 85.18% at 2 weeks in minimally invasive group and surgical group respectively. No serious complication occurred in this group.ConclusionsThe combination of the oxygen–ozone with collagenase shows significant reductions in pain and improvements in function at 3 and 12 months, it can be considered as an option for the treatment of non-contained lumbar disc herniation instead of surgery.     

椎-基底动脉供血不足性眩晕ENG和ABR检测的意义

目的 探讨椎-基底动脉供血不足性眩晕患者眼震电图(ENG)和听性脑干反应(ABR)的改变及其临床意义.方法 对60例椎-基底动脉供血不足性眩晕患者行眼震电图和听性脑干反应检测,观察视眼动系统反应、自发性眼震、冷热试验、位置性眼震和ABR各参数变化.结果 椎-基底动脉供血不足性眩晕组位置性眼震强度(7.76±6.05)°/s,明显高于对照组(P＜0.01).视动中枢检查共48例(75％)出现一项或多项异常.冷热试验总慢相速度为(34.68±5.66)°/s,对照组中总慢相速度为(46.57±6.34)°/s(P ＜0.01).听性脑干反应49例异常,占81.6％.脑干下部异常者25例(41.6％),脑干上部异常者12例(20％).结论 眼震电图和听性脑干反应检查可从电生理方面有效鉴别椎-基底动脉供血不足性眩晕患者的脑干与视眼动系统异常.尤其是对内耳与脑干功能损害的鉴别,ENG和ABR可提供客观有效的帮助.     

A case report of primary mediastinal yolk sac tumor

Primary yolk sac tumor of the mediastinum is very rare. Yolk sac tumor is highly malignant, and has less chance of operation. One case, which treated in this institute since 1954, is retrospectively studied to investigate the clinical characteristics, surgical treatment and prognosis of the primary mediastinal yolk sac tumor.     

超高频皮肤整形仪治疗睑部病变826例

目的：探索一种治疗睑部皮肤病变行之有效的方法。方法；对8种826例睑部皮肤病用超高频皮肤整形仪治疗。结果：特定频段的超高频电极可使皮肤病变组织气化，操作精细，不损伤深层组织，创面新鲜，愈合后皮肤恢复正常。结论：超高频皮肤整形仪治疗睑部皮肤病变效果良好。     

排便习惯训练与儿童功能性便秘发病相关分析

为探讨排便习惯训练(DHP)对小儿功能性便秘(FC)发病的影响，并确定DHP。在预防、治疗小儿FC的作用，自行拟订DHP情况调查问卷，由经专题培训的人员，对38例FC患儿观察组及92名健康对照组小儿家长依据问卷内容进行详细调查，有关数据经U检验行统计学处理。结果表明，观察组未进行DHP者占42．10％，不规范DHP者57．90％。对照组小儿自6个月开始全部进行规范DHP，其中排软便者84．78％，条形干便14．13％，球形干便1．09％，患FC者仅1例。两组小儿膳食结构差异无显著性。表明小儿FC的发病与未曾进行或不规范的DHP高度相关，DHP在预防和治疗小儿FC时有重要作用。