lncRNA ANCR调节骨质疏松大鼠脂肪源性干细胞分化成骨细胞及对Wnt信号通路的作用机制

目的探讨lncRNA ANCR调节骨质疏松(osteoporosis,OP)大鼠脂肪源性干细胞分化成骨细胞及对Wnt信号通路的作用机制。方法将lncRNA ANCR siRNA慢病毒载体及慢病毒空载体分别转染至脂肪源性干细胞,分为siRNA组、空载体组、干细胞组。分别通过碱性磷酸酶染色观察ALP染色效果、Western blot法检测Wnt/β-catenin蛋白水平、茜素红染色实验检测成骨染色效果、免疫组化法检测Wnt/β-catenin信号阳性表达的差异性。结果与空载体组、干细胞组相比较,siRNA组细胞中ALP染色显著加深(P均0.05),成骨染色更为显著(P均0.05);与空载体组,干细胞组相比较,siRNA组细胞Wnt/β-catenin蛋白表达显著上升(P均0.05),Wnt/β-catenin阳性数显著升高(P均0.05);空载体组与干细胞组比较,细胞中Wnt/β-catenin蛋白、ALP表达水平、Wnt/β-catenin阳性数相近,无显著差异(P0.05)。结论 lncRNA ANCR的表达水平与骨质疏松大鼠体内脂肪源性干细胞的分化存在显著相关性,下调lncRNA ANCR能够促进脂肪源性干细胞向成骨细胞分化,其作用机制可能与调控Wnt/β-catenin的表达有关。     

Reconstruction of Soft Tissue Defects in the Hand with a Free Anterolateral Thigh Deep Fascia Flap

ObjectiveTo report our experience in the reconstruction of soft tissue defects in the hand with a free anterolateral thigh deep fascia flap and describe the clinical outcomes.MethodsThis study was a retrospective trial. From November 2016 to January 2020, six patients (four men and two women) with soft tissue defects in the hand were included in this study. The average age of the patients was 33.7 ± 12.7 years (range, 20 to 50 years). All patients underwent reconstructions with free anterolateral thigh deep fascia flaps. Relevant clinical characteristics were recorded prior to surgery. The size and thickness of the deep fascia flap and the thickness of the skin were measured intraoperatively. The survival of the flaps and skin grafts and the occurrence of infection were recorded after the operation. At follow‐up, donor site complications and postoperative effects were evaluated according to the outcome satisfaction scale. The pain in the injured hand was assessed using the visual analog scale.ResultsThe average body mass index (BMI) was 26.6 ± 1.7 kg/m² (range, 23.9 to 28.7 kg/m²). The defect sizes ranged from 5 cm × 5 cm to 13 cm × 8 cm (average, 53.1 ± 27.9 cm²). The six anterolateral thigh deep fascia flaps ranged from 7 cm × 6 cm to 14 cm × 9 cm in size (average, 71.8 ± 29.1 cm²). The thicknesses of skin ranged from 25 mm to 40 mm (average, 32.5 ± 4.8 mm), and the thicknesses of the deep fascia flaps ranged from 2 mm to 3 mm (average, 2.5 ± 0.5 mm). After the operation, the blood supply of the deep fascia flap was normal in all cases. The second‐stage skin grafts of most patients survived completely. The skin graft in one case was partially necrotic and healed after a dressing change. No infection occurred. At follow‐up (average, 16.3 ± 6.9 months), there was only a linear scar and no loss of sensation at the donor site of each patient. According to the outcome satisfaction scale, the outcome satisfaction score ranged from 6 to 8 (average, 7.2 ± 0.9), all of which were satisfactory. Apart from one patient who reported mild pain, all the other patients reported no pain. Three typical cases are presented in this article.ConclusionsThe free anterolateral thigh deep fascia flap, which is suitable for reconstruction of soft tissue defects in the hand, can provide very good outcomes both functionally and aesthetically.     

JNK信号转导通路对白细胞介素-1β促肝星状细胞增殖的调控作用

目的：探讨JNK信号转导通路在白细胞介素- 1β（IL-1β）介导的促肝星状细胞（HSCs）增殖中的作用。方法:应用 Western印迹法检测JNK的活化程度。应用活细胞计数试剂盒-CCK-8检测HSCs增殖并观察JNK 特异性阻断剂SP600125对IL-1β促HSCs增殖的影响。结果：IL-1β有明 显促大鼠HSCs增殖作用，而经JNK特异性阻断剂 SP600125预处理后，IL-1β促HSCs增殖作用 受到抑制（1.560±0.110 vs 1.427±0.113，P<0.05）。IL-1β以时间依赖 方式激活JNK。IL-1β作用HSCs后0、5、15、30、60和120 min，JNK 活性分别为0.982±0 .299、1.501±0.720、2.133±0.882、3.360±0.452、2.181±0.789、1.385±0 .368。结论:IL-1β可刺激HSCs增殖，细胞内JNK信号转导通路参与了 IL-1β促HSCs增殖作用。     

卡维地洛改善高血压患者的血管内皮功能

目的：探讨卡维地洛对原发性高血压患血管内皮功能的影响。方法：用高分辨率超声检测经美托洛尔或卡维地洛治疗12wk前后的高血压患肱动脉流量介导的舒张活性（FMD）和硝酸甘油介导的舒张活性（NTGMD）的变化。并以18例健康作为对照；同时采用酶联免疫检测法（ELISA）测定卡维地洛治疗前后高血压患血浆氧化低密度脂蛋白（ox-LDL)的变化。结果：健康对照组的FMD显高于高血压患美托洛尔或卡维地洛治疗前的FMD，而3组间NTGMD的差异无显统计学意义。卡维地洛组治疗后FMD显性升高，NTGMD无显性改变，卡维地洛组治疗后血浆ox-LDL显降低。结论：卡维地洛可能通过其抗氧化活性改善高血压患的血管内皮依赖性舒张功能。     

自然流产妇女血清MDA、SOD、VitE的测定及意义

目的:通过对自然流产妇女血清氧化应激指标———超氧化物歧化酶(SOD)、丙二醛(MDA)及维生素E(V itE)的测定,分析三个指标在自然流产发病机制中的意义。方法:用化学比色法对30例自然流产妇女及20例正常早孕组妇女血清中SOD、MDA及V it E进行测定。结果:与正常早孕妇女相比,自然流产妇女血清中MDA水平升高(P<0.05),SOD、V it E水平却下降(均P<0.05)。结论:上述三个指标对于预防及治疗自然流产有重要意义。     

B超监测在子宫畸形合并早孕的人工流产术中的应用

目的:评价B超在子宫畸形合并早孕的人工流产术中的应用价值。方法:收集2003年8月~2004年8月妇产科门诊1 741例人工流产妇女中48例子宫畸形合并早孕患者,在B超监测下行人工流产术。结果:48例手术均为1次成功,无漏吸、吸宫不全、子宫穿孔等并发症发生,手术时间短、出血少。结论:对于子宫畸形合并早孕患者应用B超监测完成人工流产手术安全,病人痛苦小,值得推广。     

吉兰-巴雷综合征相关空肠弯曲菌的蛋白质谱特征分析

目的 分析吉兰-巴雷综合征(GBS)相关空肠弯曲菌(Cj)与非GBS相关Cj的蛋白质谱特征。方法 利用双向电泳对这两类Cj各8株菌的全菌蛋白进行分离,比较蛋白质谱之间的差异,并对差异蛋白进行基质辅助激光解吸离子化飞行时间质谱(MALDI-TOF)分析。结果 比较发现20个差异蛋白,质谱分析鉴定出17个蛋白质,包括wlaX蛋白,参与能量代谢的苹果酸脱氢酶、磷酸丙糖异构酶、镍-铁还原酶小亚单位、半胱氨酸合成酶及支链氨基酸氨基转移酶,参与细胞加工处理过程的热休克蛋白、铁吸收ABC转运系统周质铁结合蛋白、烷基过氧化氢还原酶以及与细胞表面结构有关的鞭毛蛋白、UDP-N-乙酰烯醇式丙酮酸葡萄糖胺还原酶等。结论 wlaX蛋白可能与致GBS相关脂多糖的独特结构合成或细菌的毒力有关,wlaX蛋白及鞭毛蛋白有可能为GBS相关Cj的特征蛋白。