类风湿关节炎合并肺间质纤维化患者证候特征及影响因素分析*

目的分析类风湿关节炎合并肺间质纤维化（RA-IPF）的中医证候特征及其相关影响因素。方法 采用病例对照的研究方法,通过电子病历系统搜集RA患者的基本信息、实验室指标、证候分型等临床资料,比较RA-IPF患者与单纯RA患者之间存在的差异,并分析RA-IPF的相关影响因素。结果 在2 788例RA患者中并发IPF者115例。与单纯RA相比,RA-IPF患者男性比例较多、年龄较大、血沉（ESR）加快、血D-二聚体增多、血RF-IgA升高、RF-IgM升高、RF-IgG升高、抗U1-RNP抗体升高、抗Jo-1抗体升高。结论 RA-IPF可能与患者的性别、年龄、ESR、D-二聚体、RF-IgA、RF-IgM、RF-IgG、抗U1-RNP抗体、抗Jo-1抗体有关;高龄是RA合并IPF的危险因素。     

高血压性脑出血CT诊断——附18例分析与讨论

一、引言CT 诊断脑出血是一种迅速而可靠的检查方法。新鲜出血所形成的血肿,其密度明显地高于正常脑组织,为55～95HU。甚而血肿小于1 cm 者也可被CT 所探及。根据临床分析,脑内出血除外伤外,最常见的原因是高血压性血管病变所致。至于动脉瘤破裂或动一静脉畸形所引起的血肿在临床上较少见,更为少见的原因是静脉性血栓、淀粉样血管病变,胶原性血管病变,抗凝治疗、原发或转移性肿瘤的出血。     

反义凝血酶受体基因序列对大鼠血管平滑肌细胞增殖的影响

为了研究凝血酶受体在血管平滑肌细胞增埴中的作用 ,探讨反义凝血酶受体基因序列对大鼠血管平滑肌细胞增殖的影响 ,通过培养SD大鼠胸主动脉血管平滑肌细胞 ,以3 H TdR掺入率作为评价反义凝血酶受体基因序列抑制血管平滑肌细胞增殖的指标 ;用反转录聚合酶链反应检测反义凝血酶受体基因序列抑制血管平滑肌细胞凝血酶受体mRNA的表达 ;用Western blot检测反义凝血酶受体基因序列抑制血管平滑肌细胞凝血酶受体蛋白质的表达 ;用3 H 肌醇掺入率检测反义凝血酶受体序列抑制血管平滑肌细胞磷酸肌醇代谢的影响。结果发现 ,反义凝血酶受体基因序列明显抑制血管平滑肌细胞的增殖 (与对照组相比 ,P <0 .0 5 ) ;明显抑制血管平滑肌细胞的凝血酶受体mRNA和蛋白的表达 ;明显抑制了血管平滑肌细胞磷酸肌醇代谢。提示反义凝血酶受体基因序列明显抑制血管平滑肌细胞的增殖 ;凝血酶受体反义序列明显抑制血管平滑肌细胞的增殖是通过抑制凝血酶受体基因的表达 (特别是通过抑制DNA、mRNA和蛋白的表达 ) ,抑制细胞内信号传递来完成     

主动脉内球囊反搏泵的临床应用进展

主动脉内球囊反搏泵（IABP）是一项临床上常用的治疗手段,现已广泛应用于急性心肌梗死合并心源性休克、血流动力学不稳定的高危经皮冠状动脉介入治疗、冠状动脉旁路移植术等临床情况。最近一些研究对IABP的应用提出质疑,但我们综合分析认为IABP的应用是有价值的。     

Effectiveness of aspartate aminotransferase as a marker of periodontal disease in children and adolescents

BackgroundBiochemical markers from saliva and gingival crevicular fluid are of interest in preventing periodontal disease. We investigated the possibility of detecting aspartate aminotransferase (AST) levels in children and teenagers, as well as the usefulness of the addition of measurements of N-bezyoyl-DL-arginine-2-naphthylamide (BANA) activity and AST release during regular oral check-ups.Subjects and methodsFifty-four children (33 males; 8–15 years) from the pediatric dental clinic of Hiroshima University Hospital were enrolled. Periodontal conditions were investigated using scores of the community periodontal index (CPI), BANA and AST. Bacterial species were also identified.ResultsIn 51 eligible subjects, 64.7% were CPI 1 and 35.3% were CPI 2, and these subjects were diagnosed as having gingivitis. A high BANA score was increased in the CPI 2 group (p = 0.022) and the AST score showed a similar trend. All scores of BANA and AST were found in the same CPI. The number of subjects with higher scores of BANA and/or AST increased with increasing age.ConclusionsIt was possible to measure AST levels in children and teenagers. It is suggested that we provide more specific information to children and their guardian using BANA and AST. Measuring biomarkers during regular oral check-ups could lead to an early diagnosis and intervention to avoid the progression of periodontitis during the childhood and teenage years.     

血管内皮生长因子、微血管密度在子宫平滑肌瘤中的表达意义

Objective:To investigate vascular endothelial growth factor(VEGF) expression and microvessel density(MVD) on the fibromyoma uteri treated with mifepristone.Methods:VEGF expression and MVD were counted on 60 cases of the fibromyoma uteri by SP method,including 40 cases of mifepristone treated and 20 cases of untreated patients as controlled group.Results:VEGF positive expression rate and MVD in treated group were 37.5% and 9.90 ± 5.95,which were lower than those in controlled group(80% and 16.36 ± 2.07;P < 0.05).Meanwhile,in treated group,those in marked shrink in tumor size sub-group were lower than in not obvious sub-group(12.0% and 7.89 ± 4.36 vs.80% and 11.29 ± 3.10;P < 0.05).Conclusion:VEGF and MVD expression decreases in the fibromyoma uteri after treatment with mifepristone,suggesting that mifepristone could inhibit angiogenesis and blood supply resulting in tumor shrink.     

MTA1基因在非小细胞肺癌中的表达及其意义

背景与目的转移相关基因(metastasis-associated gene 1,MTA1)是近年来研究较深入的肿瘤浸润转移基因,在多种肿瘤细胞系中表达,与肿瘤的侵袭和转移密切相关。本研究目的是探讨MTA1基因的表达与非小细胞肺癌的浸润、转移关系。方法建立测定MTA1mRNA表达的巢式逆转录聚合酶链反应(RT-PCR)最适条件,在半定量水平测定42例非小细胞肺癌标本癌组织、癌旁组织、正常组织和淋巴结及20例肺良性疾病组织MTA1mRNA表达的相对水平,分析其与临床和组织病理学特征的关系。结果MTA1mRNA在非小细胞肺癌组织(1.50±0.26)及转移淋巴结(1.88±0.35)中的平均表达水平高于正常组织(1.02±0.17)和良性疾病组织(0.90±0.15)(P<0.01),癌组织中MTA1的表达(1.50±0.26)高于癌旁组织(1.09±0.16)(P<0.01),淋巴结转移组MTA1的表达(1.88±0.35)高于无淋巴结转移组(1.40±0.36)(P<0.01),非小细胞肺组织中MTA1mRNA高表达率与临床分期、T分期和N分期呈正相关关系。42例非小细胞肺癌组织中,19例MTA1mRNA高表达,占45.2%;19例有转移的淋巴结中,16例高表达占84.2%。MTA1在癌组织中的表达与患者年龄、性别、肿瘤类型无关(P>0.05)。结论MTA1mRNA过度表达与非小细胞肺癌浸润和转移密切相关。MTA1mRNA的过度表达可能是评价非小细胞肺癌的恶性程度、转移的潜在指标。