前降钙素对新生儿重症感染的诊断价值

目的评价前降钙素(procalcitonin,PCT)对新生儿重症细菌感染的诊断价值。方法应用免疫发光法对30例重症感染新生儿和30例非感染对照组病人在入院时进行血清PCT的定量检测,并与C反应蛋白(CRP)和白细胞计数(WBC)进行比较。同时检测其敏感度、特异性、预计值和ROC曲线下面积(AUC)。结果重症细菌感染病人在入院时血PCT质量浓度明显增高(中位数7.82μg/L,范围0.97~256.81μg/L),而非感染对照组为0.16μg/L(0.10~1.49μg/L)。PCT、CRP和WBC的ROC曲线下面积分别为0.99、0.62和0.60。预计重症感染的最佳截断值为:PCT>2μg/L(敏感度87%、特异性和阳性预计值100%、阴性预计值88%);CRP>20 mg/L、PCT>0.9μg/L时,敏感度和阴性预计值为100%,可以确诊所有重症细菌感染病人。结论重症感染新生儿入院时的PCT血质量浓度是优于CRP和WBC的细菌感染诊断指标。     

Effect of an antidiabetic polysaccharide from Inula japonica on constipation in normal and two models of experimental constipated mice

OBJECTIVES: The aim of the present study was to investigate the effect of an antidiabetic polysaccharide (IJP) from Inula japonica on gastrointestinal transit in normal mice and on constipation in two models of constipated mice. METHODS: Two models of constipation in mice were respectively induced by fasted water for 4 days or induced by diphenoxylate. The normal and constipated mice were administered IJP once at doses of 100 and 400 mg/kg (p.o.), the gastrointestinal vermicular motion, start time of defecation, number and weight of stool were investigated. RESULTS: After administration of IJP, the gastrointestinal propulsive rate was increased by 9.79% and 10.42%, the start time of defecation was shortened by 37.27% and 44.06%, the number of feces increased by 115.4% and 130.8% in normal mice. In fasting‐water constipated mice, the start time of defecation was shortened by 9.69% and 30.52% by IJP, defecation granules raised by 22.09% and 39.53%, wet feces weights were increased by 23.50% and 39.14% compared with the untreated constipated mice. In diphenoxylate‐induced mice, the start time of defecation was shortened by 25.48% and 28.13%, defecation granules raised by 100.0% and 118.0%. CONCLUSIONS: Consumption of IJP effectively improved bowel movement, stool output observed in this study. IJP may be practical in relieving constipation in the elderly diabetic population. Copyright © 2010 John Wiley & Sons, Ltd.     

Correlation of human epidermal growth factor receptor protein expression and colorectal cancer

AIM: To investigate the correlation between human epidermal growth factor receptor (HER-2) protein expression and colorectal cancer (CRC) using a case-control study and meta-analysis.METHODS: Tumor tissue specimens from 162 CRC patients were selected for the case group. Fifty cases were randomly selected, and normal CRC tissue at least 10 cm away from the tumor margins of these cases was used to generate the control group. The expression of the HER-2 protein in the 162 CRC tissue samples and the 50 adjacent normal mucosa tissue samples was detected via immunohistochemistry. The experimental data were analyzed using SPSS 18.0 software, and R software version 3.1.0 was utilized for further verification.RESULTS: The expression of HER-2 protein in the 162 CRC tissue samples was significantly higher than in the normal tissue specimens. The data showed that the expression of HER-2 in CRC was related to the Dukes’ stage, the depth of invasion and lymph node metastasis. The HER-2-positive patients had lower 3- and 5-year OS rates than the HER-2-negative patients, but there was no significant difference. However, there was a statistically significant difference in the 3- and 5-year disease-free survival (DFS) rates of HER-2-positive and HER-2-negative patients. The results of the meta-analysis showed that the expression of HER-2 in CRC patients was statistically significantly increased over that of healthy people. The 3-year DFS rate in HER-2-positive patients was markedly lower than that in HER-2-negative patients.CONCLUSION: Down-regulation of HER-2 expression might be a dependable strategy for CRC therapy.     

刀形针刀松解结合曲安奈德注射治疗手指狭窄性腱鞘炎31例

目的：观察刀形针刀松解结合曲安奈德注射治疗手指狭窄性腱鞘炎的临床效果.方法：31例患狭窄性腱鞘炎患者共35指采用刀形针刀行腱鞘闭合松解并注射曲安奈德治疗前后对比,评价疗效.结果：一次治愈24例,二次治愈7例.疗效评价优27例占87.1%,良3例占9.7%,总有效率96.8%.结论：刀形针刀松解结合曲安奈德注射治疗手指狭窄性腱鞘炎创伤小、无疤痕、松解充分、复发率小、痛苦少,有临床应用价值.     

高血糖大鼠蓝斑核神经原一氧化氮合酶的改变

目的观察高血糖大鼠蓝斑核NOS阳性反应物的变化。方法用腹腔注射四氧嘧啶法建立高血糖大鼠动物模型。用酶组织化学和图像分析方法观察大鼠蓝斑核NOS细胞的变化。结果高血糖大鼠蓝斑核NOS细胞阳性反应物减弱(P<0.01)。结论 NOS阳性反应物减少与高血糖的发生、发展有关。     

转化生长因子β1对骨髓干细胞分化类髓核细胞的影响

背景:转化生长因子β1是骨髓间充质干细胞分化为类髓核细胞的首选生长因子,适当浓度能诱导骨髓间充质干细胞的增殖和分化。目的:观察不同质量浓度转化生长因子β1对大鼠骨髓间充质干细胞体外向类髓核细胞分化的影响,优化骨髓间充质干细胞体外培养条件。方法:取成年大鼠股骨骨髓,体外培养、纯化。取第3代成年大鼠骨髓间充质干细胞,实验组用加入不同质量浓度转化生长因子β1(0,1,10,20μg/L)的HG-DMEM无血清培养液诱导3,7,14,21d;对照组普通状态下用含体积分数为10%胎牛血清的HG-DMEM培养液自然分化。结果与结论:10μg/L转化生长因子β1诱导液诱导的骨髓间充质干细胞蛋白聚糖与Ⅱ型胶原蛋白水平明显高于其他实验组和对照组(P<0.01)。各实验组14d时蛋白聚糖的表达均较3,7,21d时高(P<0.01)。对照组各时间点蛋白聚糖Aggrecan及Ⅱ型胶原表达均呈阴性。提示10μg/L的转化生长因子β1能提高大鼠骨髓间充质干细胞体外诱导分化类髓核的数量,从而使骨髓间充质干细胞发挥更高的治疗效率。     

表达大鼠IL-2、B7-1基因CBRH7919细胞的建立

目的:研究携带大鼠IL-2、B7-1目的基因的CBRH7919细胞(即CBRH7919/IL-2/B7-1)体外表达目的基因的能力,方法:RT-PCR扩增Wistar大鼠IL-2、B7-1目的基因,分别克隆至重组逆转录病毒载体pBaBe-puro及pMSCV-neo,构建逆转录病毒载体pBaBe-puro-IL-2及p...     

疏肝健脾方药对NASH大鼠心脏舒张功能及炎症因子的影响

目的:探讨疏肝健脾方药对非酒精性脂肪性肝炎(nonalcoholic steatosis hepatitis,NASH)合并心脏舒张功能不全大鼠干预的作用机制.方法:采用高脂饲料喂养复制NASH大鼠实验模型,各药物组分别灌服疏肝方、健脾方和综合方(疏肝方和健脾方的合方)进行干预,16 wk后用Visual Sonics...     

自制羊膜载体复合物植入表皮细胞修复全层皮肤缺损

摘要 背景：利用组织工程和细胞培养技术研制的复合皮肤距理想的人工皮肤尚有一定的差距,如局部皮肤薄弱、抗拉及抗摩擦能力差、无皮肤附属器等。 目的：观察含碱性成纤维细胞生长因子和维生素C的羊膜载体复合物对皮肤缺损局部真皮组织的修复作用,寻找真皮组织修复重建促进表皮生长的最佳时期。 方法：取新西兰大耳白兔36只,在每只背部建立皮肤全层缺损模型3处,分别植入含有缓释碱性成纤维细胞生长因子和维生素C羊膜载体复合物、单纯羊膜载体复合物及油纱覆盖。随机分为3组,分别于术后21,14,7 d植入自体表皮细胞混合液,1周后行大体、组织学、免疫组织化学、墨汁灌注等观察。 结果与结论：术后21 d植入表皮细胞组皮肤缺损局部新生皮肤瘢痕轻、形态结构较满意,创面愈合速度和质量均优于术后14,7 d 植入表皮细胞组,并且植入含碱性成纤维细胞生长因子和维生素C羊膜载体复合物组创面皮肤新生真皮组织完全被新生的表皮覆盖,而且表皮几乎均变为复层,其生长速度及质量优于单纯羊膜载体复合物移植组和油纱覆盖组。说明含有碱性成纤维细胞生长因子和维生素C的羊膜载体复合物植入皮肤全层缺损后,能加速真皮的修复和重建;真皮组织修复增生晚期和重建初期为促进表皮生长的最佳时期。 关键词：羊膜载体复合物;表皮细胞;碱性成纤维细胞生长因子;皮肤创伤愈合;真皮 doi:10.3969/j.issn.1673-8225.2011.16.003     

婴幼儿运动发育迟缓的头颅MRI表现及病因分析

目的分析婴幼儿运动发育迟缓的发病原因与头颅MRI表现。方法从344例临床确诊为运动发育迟缓的婴幼儿中随机抽取172例和作为对照的正常婴幼儿20例,均经SE序列MRI检查,包括横断面T1WI、T2WI、T2FLAIR,冠状面T2WI及矢状面T1WI平扫。统计分析是以χ2检验并利用统计软件SPSS13.0进行的。P值小于0.05为显著性差异。结果致病原因中的脑白质髓鞘化异常、脑外间隙增宽和脑室增大、缺氧缺血性脑病后遗改变的χ2值均大于等于χ20.01、P值均小于等于0.01,在统计学上有显著性意义,发病危险因素排序依次为脑白质髓鞘化异常、脑外间隙增宽和脑室增大、缺氧缺血性脑病后遗改变。皮层发育异常、胼胝体发育不良、脑畸形的χ2值均大于等于χ20.05、P值均小于等于0.05,在统计学上有意义。结论脑损伤所致的脑白质髓鞘化异常、脑外间隙增宽和脑室增大、缺氧缺血性脑病后遗改变、皮层发育异常、胼胝体发育不良、脑畸形是婴幼儿运动发育迟缓的主要颅内原因,尤其是前四项。头颅普通MRI检查能清晰地显示本病的颅内异常征象,为早期诊断和评估以及疗效随访提供重要信息。对于平扫未见明确异常者,需要深入地查找其他病因。