Associations between venous thromboembolism onset,D-dimer,and soluble fibrin monomer complex after total knee arthroplasty

Growth factors such as nerve growth factor (NGF) and insulin-like growth factor-1 (IGF-1) have been shown to play a role in the healing process of nerve injury. Recent researches have also shown that oxytocin administration activates these growth factors of importance for the healing of nerve tissue. The objective of the present study was to evaluate the effects of oxytocin on peripheral nerve regeneration in rats. Twenty-four male Sprague-Dawley rats were underwent transection damage model on the right sciatic nerve and defective damage model on the left sciatic nerve. The animals were assigned to one of two groups: control group or treatment group (received 80 mg/kg oxytocin intraperitoneally for 12 weeks). The sciatic nerve was examined, both functionally (on the basis of climbing platform test) and histologically (on the basis of axon count), 3, 6, 9, and 12 weeks after the injury. Also, stereomicroscopic and electrophysiological evaluations were carried out. Significantly greater improvements in electrophysiological recordings and improved functional outcome measures were presented in the treatment group at 12-week follow-up. Stereomicroscopic examinations disclosed prominent increases in vascularization on proximal cut edges in the oxytocin group in comparison with the control group. Higher axon counts were also found in this group. Intraperitoneal oxytocin administration resulted in accelerated functional, histological, and electrophysiological recovery after different sciatic injury models in rats.     

Co-culture with periodontal ligament stem cells enhanced osteoblastic differentiation of MC3T3-E1 cells and osteoclastic differentiation of RAW264.7 cells

Objectives: Periodontal ligament stem cells (PDLSCs) are characterized by having multipotential differentiation and immunoregulatory properties, which are the main mechanisms of PDLSCs-mediated periodontal regeneration. Periodontal or bone regeneration requires coordination of osteoblast and osteoclast, however, very little is known about the interactions between PDLSCs and osteoblast-like cells or osteoclast precursors. In this study, the indirect co-culture approach was introduced to preliminarily elucidate the effects of PDLSCs on differentiation of osteoblast-like cells and osteoclast precursors in vitro. Materials and methods: Human PDLSCs were obtained from premolars extracted and their stemness was identified in terms of their colony-forming ability, proliferative capacity, cell surface epitopes and multi-lineage differentiation potentials. A noncontact co-culture system of PDLSCs and preosteoblastic cell line MC3T3-E1 or osteoclast precursor cell line RAW264.7 was established, and osteoblastic differentiation of MC3T3-E1 and osteoclastic differentiation of RAW264.7 were evaluated. Results: PDLSCs exhibited features of mesenchymal stem cells. Further investigation through indirect co-culture system showed that PDLSCs enhanced ALP activity, expressions of ALP, Runx2, BSP, OPN mRNA and BSP, OPN proteins and mineralization matrix deposition in MC3T3-E1. Meanwhile, they improved maturation of osteoclasts and expressions of TRAP, CSTK, TRAF6 mRNA and TRAP, TRAF6 proteins in RAW264.7. Conclusions: PDLSCs stimulates osteoblastic differentiation of osteoblast precursors and osteoclastic differentiation of osteoclast precursors, at least partially, in a paracrine fasion.     

线粒体DNA变异与肥厚型心肌病

目的肥厚型心肌病（hypertrophiccardiomyopathy,HCM）是指未伴心室扩张的不明原因左室肥厚,以室间隔非对称性肥厚常见。HCM多由编码肌小节蛋白的基因突变所致;有少部分患者由线粒体DNA突变引起,呈母系遗传。线粒体DNA变异导致HCM的分子机制可能与引起的线粒体结构功能改变,造成氧化磷酸化缺陷,ATP合成不足。本文着重对与HCM相关的mtDNA变异位点作一综述。     

牙龈卟啉单胞菌对血管内膜粘附因子和金属蛋白酶的影响(英文)

目的:观察牙龈卟啉单胞菌(Pg,为牙周病的主要致病菌)对血管内膜粘附因子和金属蛋白酶的影响。方法:大鼠120只,按数字表随机分为空白对照组、Pg低剂量组(2ml)与Pg高剂量组(5ml),各40只,后两组每3d分别接受相应剂量Pg肌注,且均不进行抗生素干预,历时12周。取其静脉血,检测三组血液中的细胞间粘附分子-1(ICAM-1)、血管细胞粘附分子-1(VCAM-1)、基质金属蛋白酶(MMP)-3及MMP-9的水平。结果:与空白对照组相比,Pg低剂量组与高剂量组大鼠静脉血中ICAM-1[(175.79±14.30)ng/ml比(182.62±15.07)ng/ml、(189.39±14.93)ng/ml]、VCAM-1[(256.49±37.17)ng/ml比(271.58±32.85)ng/ml、(286.66±30.66)ng/ml]、MMP-3[(3.23±0.69)ng/ml比(3.61±0.82)ng/ml、(3.97±0.83)ng/ml]及MMP-9[(1.30±0.39)mg/L比(1.48±0.39)mg/L、(1.67±0.45)mg/L]含量显著增加(P0.05或0.01),且与Pg低剂量组相比,Pg高剂量组上述因子含量显著增加(P0.05)。结论:牙龈卟啉单胞菌可引起大鼠血清中血管内膜粘附因子和金属蛋白酶含量的显著增加,可加剧冠心病的病理演变。     

HER2与胃癌的研究进展

本文综述了HER2与胃癌相关的研究进展,介绍了HER2的结构、相关信号途径、在胃癌中的检测方法和检出率以及人源化抗HER2单克隆抗体曲妥珠单抗与胃癌治疗、HER2与胃癌预后的关系。研究显示HER2阳性是胃癌预后不良的重要因子,曲妥珠单抗与化疗药物联合应用是HER2阳性进展期胃和胃食管连接部癌以及伴肝转移患者治疗的新选择,可明显改善患者预后。     

胸椎旁神经阻滞与硬膜外麻醉对非气管插管肺叶切除术患者镇痛和免疫的影响*

目的?比较胸椎旁神经阻滞（TPVB）和硬膜外阻滞（EB）对非气管插管肺叶切除术患者镇痛和免疫的影响。方法?选取2019年1月—2019年6月在青岛大学附属青岛市市立医院东院区行胸腔镜下肺叶切除术的120例非小细胞肺癌患者,用随机数字表法分为TPVB组、EB组和全身麻醉（GA）组。比较各组患者围手术期镇痛和芬太尼追加量情况、细胞免疫及体液免疫水平。结果?3组术后1?h、2?h、6?h、12?h、24?h和48?h VAS评分在不同时间、不同组间及变化趋势上有差异（P?<0.05）。3组芬太尼追加量比较,差异有统计学意义（P?<0.05）,GA组高于TPVB组、EB组（P?<0.05）。3组术后第1天和第3天CD3+CD4+、CD3+CD8+水平在不同时间、不同组间有差异（P?<0.05）,变化趋势无差异（P?>0.05）。3组术后第1天和第3天CD3-CD16+CD56+、CD19+水平在不同时间、不同组间及变化趋势上无差异（P?>0.05）。3组术后第1天和第3天IgG、IgM、IgA在不同时间有差异（P?<0.05）,不同组间及变化趋势上无差异（P?>0.05）。与EB组比较,TPVB组患者围手术期低血压发生率降低（P?<0.05）。结论?对于非气管插管肺叶切除术患者,TPVB和EB均能提供有效的围手术期镇痛;同时TPVB术后镇痛时间更持久,术后T淋巴细胞免疫抑制减轻。     

成人不同垂直骨面型颧牙槽嵴区骨皮质密度分析

目的 通过三维重建技术对成人颧牙槽嵴区骨皮质密度进行测量分析,比较不同骨面型间颧牙槽嵴区骨皮质密度以及不同位点间骨皮质密度的差异。方法 选取成人患者螺旋 CT 图像60例（男28例,女32例）进行三维重建,根据下颌平面角( FH-MP) 的大小分为低角、均角、高角3组,分别测量上颌左侧颧牙槽嵴区不同层面基准线上方13、15、17 mm处的骨皮质密度,采用 SPSS 17.0软件包进行数据分析。结果 颧牙槽嵴区骨密度在性别之间无统计学差异;在不同垂直骨面型间存在统计学差异（P<0.05),其中,低角组[(1331.65±185.70) HU]的骨密度最高,高角组[(1245.62±207.63) HU]最低;在不同垂直骨面型组的不同位点之间骨密度存在统计学差异（P<0.05),各组骨密度的集中区域是,高角组位于第一磨牙近中颊根层面,均角组位于近、远中颊根之间,低角组位于第一磨牙远中颊根层面。结论 成人不同垂直骨面型间颧牙槽嵴区骨皮质的密度以及位点分布存在显著差异,该研究为成人选择微种植支抗钉的适宜部位,保证正畸治疗中支抗钉的稳定性提供了参考依据。     

慢性乙型肝炎患者脂联素和瘦素检测的临床意义

目的探讨CHB患者血清脂联素（ADPN）、瘦素（LEP）水平的关系及意义。方法检测52例CHB患者、51例HBV携带者以及46例健康体检者血清中脂联素、瘦素的水平,并测定ALT、AST水平,对脂联素、瘦素和肝功能损伤程度的相关性进行分析。结果 HBV携带者组ADPN水平较CHB患者组、对照组高（P〈0.05）,CHB组患者与对照组组间ADPN水平无明显差异。CHB患者组LEP水平较HBV携带者组、对照组高（P〈0.05）;HBV携带者组与对照组组间LEP水平无明显差异。3组患者的ADPN、LEP水平均呈现出女高男低的现象（P〈0.05）。CHB组患者AST、ALT水平均较HBV携带者组、对照组显著升高（P〈0.05）,HBV携带者组和对照组组间AST、ALT水平无明显差异。LEP水平与ALT和AST呈正相关（r=0.386,P〈0.05;r=0.751,P〈0.05）。结论 ADPN、LEP与CHB的发生及发展密切相关,可能是病情轻重的标志。     

Apolipoprotein C3 Gene Polymorphisms Are Not a Risk Factor for Developing Non-Alcoholic Fatty Liver Disease: A Meta-Analysis

Context:

Our objective was to evaluate the effect of gene polymorphisms of apolipoprotein C3 (APOC3) on the development of non-alcoholic fatty liver disease (NAFLD) in different populations.

Evidence Acquisition:

We performed a meta-analysis of all relevant studies published in the literature. A total of 115 clinical trials or reports were identified, but only seven trials met our inclusion criteria. A meta-analysis was performed according to the Cochrane Reviewers’ Handbook recommendations.

Results:

Five hospital-based and two population-based case-control studies were included in the final analysis. The overall frequency of APOC3 gene polymorphisms was 67.5% (1177/1745) in NAFLD and 68.8% (988/1437) in controls. The summary odds ratio for the association of gene polymorphisms of APOC3 and the risk of NAFLD was 1.03 (95% CI: 0.89-1.22),which was not statistically significant (P > 0.05).

Conclusions:

Our meta-analysis, while not ruling out possible publication bias, showed no association between gene polymorphisms of APOC3 and the risk of NAFLD development in different populations in the world.