重组腺病毒介导的p27Kip1基因治疗大鼠神经胶质瘤的实验研究

目的研究腺病毒介导的人p27Kip1基因(Ad-p27Kip1)对大鼠神经胶质瘤生长的抑制作用。方法建立大鼠胶质瘤模型,随机分成3组:Ad-p27Kip1组、Ad-LacZ和对照组,分别给予Ad-p27Kip1、Lacz重组复制缺陷性腺病毒(Ad-LacZ)和PBS瘤内注射,观察对肿瘤生长的抑制情况,并用免疫荧光法检测p27和细胞周期素(cyclinD1)的表达;原位末端标记法检测细胞凋亡情况。结果Ad-p27Kip1组肿瘤生长受到明显抑制(P<0.05),免疫荧光染色显示移植瘤p27表达明显增强(P<0.05),cyclinD1的表达受到明显抑制(P<0.05),且细胞凋亡显著多于Ad-LacZ组和对照组(P<0.05)。结论Ad-p27Kip1对大鼠胶质瘤的生长具有明显的抑制作用,其机制可能是增强p27表达、抑制cyclinD1表达。p27Kip1有望成为胶质瘤基因治疗的新策略。     

Influence of endocrine disruptors on human male fertility

It has been suggested that during the past five decades human sperm counts have declined and the incidence of testicular cancer, hypospadias and cryptorchidism has increased. Furthermore, geographical differences, with respect to these markers of male reproductive function, have been reported. According to a recent hypothesis, all these abnormalities of the male genital system do have a common cause, namely exposure to endocrine disruptors affecting the male in early fetal life. Reduced sperm production as well as congenital abnormalities of male genitalia can be evoked in laboratory animals by exposing them to chemicals with endocrine-disrupting effect, and in humans similar effects have been seen following accidental exposures to very high concentrations of these environmental toxicants. However, the evidence for association between levels of exposure found in the general population and serious adverse effects on male reproduction, including fertility, is still lacking. A recent European Union-supported study, on the effect of persistent organohalogen pollutants on human reproduction, failed to show any correlation between post-natal exposure levels and fertility. Future studies will reveal whether prenatal exposure does more strongly affect male fertility and whether genetic predisposition regulates the susceptibility of an individual to the adverse effects of endocrine disruptors.     

LRIG2基因表达与垂体腺瘤侵袭性的关系

目的探讨富含亮氨酸重复序列和免疫球蛋白样多肽2（LRIG2）基因在垂体腺瘤中的表达及其与肿瘤侵袭性的关系。方法采用逆转录聚合酶链反应（RT-PCR）技术检测临床手术切除的23例侵袭性（13例）和非侵袭性（10例）垂体腺瘤标本中，LRIG2基因在mRNA表达水平上的差异。结果RT-PCR显示在侵袭性与非侵袭性垂体腺瘤中mRNA表达的阳性率均为100％。在表达强度上，LRIG2在侵袭性垂体腺瘤中mRNA表达明显高于非侵袭性垂体腺瘤（P〈0．05）。结论LRIG2可能与垂体腺瘤的侵袭性密切相关。     

Mechanisms of Depressor Effect of Norepinephrine Injected into Subnucleus Commissuriu of Nucleus Solitarius Tractus in Rabbits

This experiment aimed to investigate the effect of adrenergic system in the subnucleus commissuriu of nucleus solitrius tractus (CNTS) on renal nerve discharges. Norepinephrine (NE)was microinjected into the CNTS of rabbits and mean arterial blood pressure (MAP) and renal nerve discharges (FRND) were synchronously recorded. The results indicated that (1) microinjection of norepinephine into the CNTS of rabbit could significantly attenuate the frequency of renal nerve discharge, and at the same time decrease markedly the mean arterial pressure. (2) Microinjection of 0.3 nmol yohimbin into CNTS had no significant influence on FRND and MAP, but could attenuate and even reverse the effects of NE on FRND and MAP. These results suggest that microinjection of NE into CNTS may activate the alpha-adrenorecptor located in CNTS and secondarily produce a depressor effect by attenuating the activity of peripheral sympathetic nervous system.     

Hepatic angiomyolipoma demonstrating similar imaging characteristics as hepatocellular carcinoma: One case report

Angiomyolipoma (AML) is a benign mesenchymal tumor that has been frequently reported in the kidney but rarely in the liver[1]. AML is composed of fat, vascular, and smooth muscle elements. Because the proportion of the constituents composed of AML are varied, hepatic AML may be clinically, radiologically and morphologically difficult to distinguish from hepatocellular carcinoma (HCC) or other hepatic lesions. Here we report a case with pathologically confirmed hepatic AML who was previously diagnosed as HCC based on imaging examinations.     

颅内大型胶质瘤的显微外科治疗

目的探讨颅内大型胶质瘤的显微外科治疗方法和效果。方法对52例直径大于5cm的颅内胶质瘤患者采用显微外科手术方治疗。结果52例患者中，显微镜下肿瘤全切44例，次全切及大部分切除8例，无手术死亡及严重并发症发生。术后随访40例，时间1～3年，肿瘤复发13例。结论显微外科治疗颅内大型胶质瘤效果显著，在保护重要功能结构区的前提下尽量全切肿瘤是确保手术疗效的关键。     

Arthroscopic Suture Repair of Peripheral Tears of Triangular Fibrocartilage Complex Using a Volar Portal

Surgical repair of a Palmer type IB triangular fibrocartilage complex (TFCC) tear can be difficult using conventional dorsal portals and it may need special repair kits. The authors describe an arthroscopic technique using an additional volar portal that allows quick access and a secure purchase of peripheral TFCC tears as well as a distinct approach to dorsal wrist structures.