利水化瘀法治疗湿热瘀阻型泌尿系结石的用药

目的:研究利水化瘀法治疗湿热瘀阻型泌尿系结石的用药规律,为中医药治疗泌尿系结石提供临床思路。方法:选取2015年4月至2016年4月在广东省第二中医院泌尿外科住院的100例324份电子病历及处方,并行回顾性分析。采用药物频数、频率统计及系统分层聚类分析方法进行分析,并探讨其用药规律。结果:9味药出现频率超过50. 00%,8味药出现频率介于25. 00%～50. 00%,13味药出现频率低于25. 00%。28味中药可以凝聚为4类。结论:利水化瘀法从下焦膀胱湿热瘀阻论治,着重使用膀胱经药物,重视其药物的归经,并且采用地道药材,因地制宜,具有岭南中医药特色。     

Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse

In this study, recombinant baculovirus carrying the microdystrophin and β-catenin genes was used to infect adipose-derived stem cells from a dystrophin-utrophin double knock-out mouse. Results showed that, after baculovirus transgene infection, microdystrophin and β-catenin genes were effectively expressed in adipose-derived stem cells from the dystrophin-utrophin double knock-out mouse. Furthermore, this transgenic expression promoted adipose-derived stem cell differentiation into muscle cells, but inhibited adipogenic differentiation. In addition, protein expression related to the microdystrophin and Wnt/β-catenin signaling pathway was upregulated. Our experimental findings indicate that baculovirus can successfully deliver the microdystrophin and β-catenin genes into adipose-derived stem cells, and the microdystrophin and Wnt/β-catenin signaling pathway plays an important role in myogenesis of adipose-derived stem cells in the dystrophin-utrophin double knock-out mouse.