Characterizing the composition of intestinal microflora by 16S rRNA gene sequencing

BACKGROUND This study determined the composition and diversity of intestinal microflora in patients with colorectal adenoma(CRA),which may provide precedence for investigating the role of intestinal microflora in the pathogenesis of colorectal tumors,the composition of intestinal microflora closely related to CRA,and further validating the possibility of intestinal flora as a biomarker of CRA.AIM To study the relationship between intestinal microflora and CRA.METHODS This is a prospective control case study from October 2014 to June 2015 involving healthy volunteers and patients with advanced CRA.High-throughput sequencing and bioinformatics analysis were used to investigate the composition and diversity of intestinal microflora in 36 healthy subjects and 49 patients with advanced CRA.Endpoints measured were operational taxonomic units of intestinal flora,as well as their abundance and diversity(αandβtypes).RESULTS In this study,the age,gender,body mass index,as well as location between controls and patients had no significant differences.The mucosa-associated gut microbiota diversity and bacterial distribution in healthy controls and colorectal adenomas were similar.The operational taxonomic unit,abundance,andαandβdiversity were all reduced in patients with CRA compared to controls.At the phylum level,the composition of intestinal microflora was comparable between patients and controls,but the abundance of Proteobacteria was increased,and Firmicutes and Bacteroides were significantly decreased(P<0.05).The increase in Halomonadaceae and Shewanella algae,and reduction in Coprococcus and Bacteroides ovatus,could serve as biomarkers of CRA.High-throughput sequencing confirms the special characteristics and diversity of intestinal microflora in healthy controls and patients with CRA.CONCLUSION The diversity of intestinal microflora was decreased in patients with CRA.An increase in Halomonadaceae and Shewanella algae are markers of CRA.     

Assessment of Triage DOA®, Status DS10, and DRIVEN-FLOW® M8-Z on-site drug screening kits for postmortem urine

Based on the screening results of mass analyses using gas chromatography- mass spectrometry (GC–MS) and liquid chromatograph-tandem mass spectrometry (LC-MS/MS), we assessed the performance of Status DS10 (Status) and DRIVEN-FLOW® M8-Z (DF8), and compared the results with those of Triage DOA® (Triage) using 356 autopsy urine samples within one month of death. The sensitivity to benzodiazepines was 0.52 in Triage, 0.59 in Status, and 0.58 in DF8 with few false-positive cases. Triage detected triazolo-derivatives more easily than DF8. DF8 detected diazepam and nitro-benzodiazepines more easily than Status and Triage, with Status performing better than Triage. However, lorazepam detection with Status was difficult. There were 11 false-positive cases for amphetamines in Triage and 12 for Status-AMP at more than one week after death, but there were no false-positive in Status-MET and DF8. Tricyclic antidepressant (TCA) was detected in five cases by mass analysis, while there were 6 false-positive cases in Triage and 10 in both Status and DF8. In the TCA false-positive cases, tricyclic psychotics such as quetiapine, chlorpromazine, and carbamazepine existed. There were 23 true-positive and 6 false-positive cases for zolpidem in DF8 without false-negative cases. The accuracy of Status and DF8 for barbiturates or opiates was almost 1, but Triage was 0.98. There were no samples containing cocaine, THC, phencyclidine, or methadone. Based on the above, we conclude that Status and DF8 are comparable or slightly better than Triage, with fewer false-positive and fewer false-negative cases, except for TCA.     

Self-induced hemopericardium using a syringe needle: A rare method of suicide

This communication highlights a rare case of suicide by self-induced hemopericardium caused by a penetrating syringe needle. A 20-year-old male, diagnosed with ulcerative colitis, severe depression and hypochondriasis, was found dead at home. There was a 23-gauge 3 cm long syringe needle, connected to a 3 cc barrel, penetrating the left side of the chest. Autopsy revealed 3 fresh puncture marks on the left side of the anterior chest with subcutaneous hemorrhage underlying the puncture marks. The needle had penetrated through the 4th intercoastal space and entered the pericardial cavity causing superficial lacerations to the anterior wall of the heart. There were two anterior pericardial perforations. A hemopericardium of 235 ml was noted. Interventricular septal branches arising from the lower half of the left anterior descending artery were lacerated. Microscopy revealed multiple fresh hemorrhages into the myocardium at the site of myocardial injuries. Toxicological analysis of blood, urine and swabs from the syringe barrel was negative. The cause of death was given as hemopericardium due to laceration of the interventricular septal branches of the left anterior descending artery due to penetration by a 23-gauge syringe needle. The circumstance was concluded as suicidal. Suicide by stab to the chest using a 23-gauge syringe needle, resulting in hemopericardium where the source of blood was the coronary artery, has not been reported in the medical literature. A forensic pathologist must be vigilant and cautious in the interpretation of similar findings especially when cardiopulmonary resuscitation and/or therapeutic intracardiac injections have been administered.     

盆底康复治疗对预防妇科恶性肿瘤患者术后尿潴留的疗效观察

目的：探讨盆底康复治疗对预防妇科恶性肿瘤术后尿潴留的疗效观察。方法选择2012年妇科恶性肿瘤术后患者68例，将上半年患者定为对照组，将下半年患者定为治疗组。对照组给予常规治疗和护理。治疗组在对照组治疗的基础上于拔尿管前3天开始使用肌肉神经生物电疗仪对患者进行盆底肌康复治疗，分别观察两组患者膀胱功能恢复情况。结果治疗组发生尿潴留的几率远低于对照组，治疗组的临床疗效明显优于对照组（P〈0.05）。结论盆底康复治疗对预防妇科恶性肿瘤术后发生尿潴留有明显疗效。     

生物安全柜高效过滤器完整性测试与分析

高效过滤的完整性是生物安全柜安全使用的重要性能,也是操作使用者高度关注和检测过程中经常遇到的问题。通过对生物安全柜高效过滤的完整性的测试和分析、评价,为《生物安全柜检测/校准方法研究》课题研究和《生物安全柜性能测评方法》的制定提供技术支持,从而保证在用生物安全柜的安全、可靠。     

Recurrent ZNF83-E293V Mutation Promotes Bladder Cancer Progression through the NF-κB Pathway via Transcriptional Dysregulation of S100A8

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玻璃纤维桩加树脂核与铸造金属桩核修复上颌前磨牙疗效观察

目的探讨玻璃纤维桩加树脂核与铸造金属桩核修复上颌前磨牙疗效。方法分析上颌前磨牙残根残冠患者52例临床资料,依据修复系统不同进行分组,玻璃纤维桩组30例和铸造金属桩组22例。结果玻璃纤维桩组保存修复成功率明显高于铸造金属桩组,差异有统计学意义(P<0.05)。结论玻璃纤维桩加树脂核修复上颌前磨牙残根残冠效果明显,预后良好,值得临床推广应用。     

宫腹腔镜联合诊治不孕症68例临床分析

目的：探讨官腹腔镜联合诊治不孕症的临床效果。方法：对本院2005年3月-2008年12月行宫腹腔镜联合诊治不孕症68例与同期单纯行官腔镜诊治不孕症60例对比,观察两种方法对不孕症的病因检出率及术后妊娠率的影响。结果：联合检查发现病变61例,阳性率为89．7％,单纯宫腔镜检查发现病变38例,阳性率为63．3％,对所有病变分别给予治疗,随访2年,联合组及对照组术后妊娠率分别为39．7％、16．7％,两组比较,差异有统计学意义,P〈0．01。结论：宫腹腔镜联合诊治不孕症,诊治一体,病因检出率高,治疗效果好,值得推广。     

沙眼衣原体感染患者的衣原体抗体和热休克蛋白60抗体的测定

目的 探讨衣原体IgG 抗体(CT-IgG)和衣原体热休克蛋白60 的IgG 抗体(C-HSP 60 IgG)与生殖道沙眼衣原体感染(GCI)的关系,探求更为准确的GCI 初筛指标及其组合.方法 采用酶联免疫吸附试验(ELISA)检测40 例GCI 患者和40 例健康体检者的CT-IgG 和C-HSP 60 IgG 的表达,并对实验结果进行单个指标和联合指标的分析.结果 GCI 患者组的40 例患者的CT-IgG 阳性为30例(75%),C-HSP 60 IgG 阳性为23 例(57.50%),与对照组的40 例健康体检者比较,对照组的CT-IgG 阳性为2 例(5%),C-HSP 60IgG 阳性为5 例(12.5%),2 组比较差异有统计学意义(P＜0.05).CT-IgG + C-HSP 60 IgG 联合组合的临床诊断价值最高,该组合的敏感度达82.5%.结论 CT-IgG 和C-HSP 60 IgG 可用于GCI 患者的临床检测,联合检测可提高CT 检测的敏感度.