Detecting the polymorphism sites of p53 and Fas genes of Han population in Zhejiang province

IN T R O D U C T IO N W ith the developm ent of hum an genom e project (H G P ) all over the w orld, ranks of nucleic acid of hum an genom e com pletely step from processing fram ew ork into com pletive one. R anks of nucleic acid of hum an genom e are co…     

Th17细胞在系统性红斑狼疮免疫炎症反应中的作用机制研究

目的从Th17/Treg、IL-17及RORγt mRNA表达3方面进行研究,探讨Th17细胞在SLE免疫炎症反应中的作用机制。方法研究对象为26例活动期SLE患者、16例非活动期SLE患者和20例正常对照,采用细胞内染色流式细胞术检测Th17/Treg,ELISA法检测IL-17及RT-PCR法检测RORγt mRNA表达,并探讨它们与SLE疾病活动性的关系。结果活动期SLE患者组Th17/Treg、IL-17及RORγt mRNA表达水平显著高于非活动期及正常对照组;非活动期SLE患者组RORγt mRNA表达显著高于正常对照组,但两组间Th17/Treg、IL-17水平无显著差异。SLE患者Th17/Treg、IL-17及RORγt mRNA表达水平均与和SLEDAI呈正相关。结论 SLE患者存在着Th17细胞高表达现象,阻断Th17细胞分化的上游或下游均可能减轻SLE的免疫炎症反应而达到治疗SLE的目的。     

Ustekinumab治疗斑块状银屑病疗效和安全性的Meta分析

目的评价Ustekinumab治疗斑块状银屑病的疗效和安全性。方法计算机检索中英文数据库,按照制定的标准纳入随机对照试验,手工查找检索到文献的参考文献。由2名研究者独立对各临床试验进行数据提取和质量评估。采用RevMan4．2．2软件进行Meta分析。结果纳入4项随机对照研究,共计2437例患者。Meta分析结果显示,Ustekinumab治疗斑块状银屑病12周获得的疗效（三项指标）优于安慰剂,但Ustekinumab90mg组和Ustekinumab45mg组的疗效无显著差异,相对危险度为0．94（95％可信区间0．82～1．07）,P=0．34;治疗28周,在皮损消退方面的疗效Ustekinumab90mg组优于Ustekinumab45mg组,相对危险度为O．89（95％可信区间0．84～0．95）,P=0．0006,在生活质量改善方面两组无显著差异,相对危险度为0．93（95％可信区间0．85～1．01）,P=0．08。最常见的不良反应是上呼吸道感染、鼻咽炎、头痛,Ustekinumab与安慰剂、Ustekinumab两个剂量之间的发生率无显著差异。结论皮下注射Ustekinumab治疗中重度斑块状银屑病安全有效。     

Reconstruction of the glans with free-skin graft applying the Bracka technique

IntroductionWe analyse our experience in the conservative surgical management of penile cancer and/or penile skin pathologies at our institution.Material and methodsWe have retrospectively reviewed all the skin grafting procedures performed in penile surgery in the last eight years. We show the indications and results of these surgical procedures and the detailed surgical technique originally described by Bracka.ResultsTen patients had several types of partial penile removal surgery followed by free-skin graft resurfacing, creating a neoglans. There were no relevant or major complications; two patients suffered partial necrosis of the skin graft. There was no local recurrence. 6 Patients returned to normal sexual activity after complete healing.ConclusionsThere is a significant number of patients with penile cancer and/or other penile skin pathologies who can undergo definitive and non-mutilating surgery with excellent oncologic, cosmetic and functional results with skin grafting.     

Estradiol biodisponible (non-SHBG-bound E2). Dosage ou calcul en relation avec la densité minérale osseuse lombaire (L1–L4) chez l'homme âgé

With age, decreased levels of bioavailable T and E₁ + E₂ are mainly the result of the increase of SHBG levels. Several studies have shown the association of low levels < 11 pg/ml (40 pM) of bioavailable estradiol with vertebral fractures and osteoporosis in older men. They used assayed E₂ Bio or calculated non-SHBG-bound E₂ calculated using the equation derived from the law of mass action for the model: two binding proteins (SHBG, Albumin) and two ligands (T, E₂) (FE₂c_II Södergard) while serum SHBG, albumin, T and E₂ are determined experimentally in every sample. The aim of the study is to evaluate the performance of E₂ Bio and FE₂c_II associated with vertebral osteoporosis on 62 men (median age: 64 years). As expected, SHBG increases markedly with age (P = 0.003) and Ttot increases with SHBG (P = 0.0003). DMO and T score are positively correlated (P = 0.086–0.048) with E₂ Bio in men with E₂ Bio < 40 pM and are not correlated (P = 0.70–0.48) in men with E₂ Bio > 40 pM. E₂ Bio (P < 0.003) is inversely associated with SHBG levels but FE₂c_II is not correlated with SHBG (P > 0.45) and with DMO or T score adjusted for body weight (P = 0.48). Considering the lack of sensitivity of calculated FE₂c_II to estimate the "non-SHBG-bound E₂", the bioavailable estradiol assay by ammonium sulfate precipitation is the preferred method for detecting elderly men in the lowest quartile for E₂ Bio and at risk for developing osteoporosis later in life.     

糖尿病大鼠皮肤的组织学改变及其对糖皮质激素药物经皮吸收的影响

探讨糖尿病大鼠皮肤改变及其对糖皮质激素药物氢化可的松经皮吸收的影响。Wistar雄性大鼠随机 分成正常组及造模1周组 (W1) 、2周组 (W2)、3周组 (W3) 和4周组 (W4), 每组6只, 采用链脲菌素 (STZ, 40 mg·kg⁻¹) 对大鼠进行糖尿病造模, 取大鼠腹部皮肤进行体外透皮扩散实验, 接收液为磷酸盐缓冲液 (PBS, pH 7.4), HPLC法测定接收液中的药物量, 计算出药物渗透速率; 将造模不同时间的大鼠皮肤组织做病理切片, 进行HE染色并观察是否存在组织学差异。结果发现, 正常组、W1、W2、W3和W4组的渗透速率分别为 (2.39 ± 1.25)、(3.22 ± 1.72)、(3.02 ± 1.89)、(3.63 ± 2.02) 和 (5.00 ± 3.36) μg·h⁻¹·cm⁻²。造模4周组与正常组之间存在显著差异   (P < 0.05), 其余各组之间均无显著性差异; 皮肤组织学观察结果显示: 造模1周时大鼠腹部皮肤改变并不明显,  1个月时表皮有变薄的现象, 2个月时这种现象变得尤为明显。与正常皮肤相比, 患糖尿病后的皮肤能引起药物经皮透过性的增加, 这与糖尿病后发生一定程度的皮肤组织学改变关系密切。因此, 提示糖尿病患者在必要时需要调整经皮给药的剂量。     

Induction of apoptosis in human cervical carcinoma HeLa cells by polymethoxylated flavone-rich Citrus grandis Osbeck (Dangyuja) leaf extract

Citrus grandis Osbeck (Dangyuja) has a high content of flavonoids with health-related properties. Although previous data have revealed the anticancer potency of some Citrus species, the underlying molecular mechanisms of this activity by leaf extracts have not been studied in detail. The purpose of this study was to evaluate the cytotoxic effects of citrus leaves on five human cancer cell lines and to determine the possible mechanisms of cell death elicited by the chloroform fraction (CF) of the Dangyuja leaf. The CF of Dangyuja strongly decreased the survival rate of HeLa cells, among the tested cell lines. CF treatment induced the down-regulation of anti-apoptotic Bcl-2 expression, resulting in the proteolytic activation of caspases and the degradation of poly (ADP–ribose) polymerase (PARP) protein. Arrested cell growth and induction of apoptosis were confirmed by flow cytometry and DNA fragmentation analysis, respectively. The major components of the CF were identified as isosinensetin, sinensetin, tetramethyl-O-isoscutellarein, nobiletin, tangeretin, and 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone by liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS). Our results suggest that the CF of Dangyuja leaves is an excellent source of functional polymethoxylated flavones, which may help prevent cervical cancer and may potentially be a useful agent for the treatment of certain malignancies.     

寻常性银屑病患者血清sCD40L水平及甲氨蝶呤的干预作用

目的研究可溶性CD40配体(sCD40L)在寻常性银屑病发病中的作用及甲氨蝶呤(MTX)对其的调节作用,探讨MTX治疗银屑病的机制。方法应用双抗体夹心酶联免疫吸附法(ELISA)检测30例寻常性银屑病患者经MTX治疗前、后外周血清中sCD40L的水平变化。结果寻常性银屑病患者血清中sCD40L的水平显著高于经MTX治疗后及正常人对照组(P<0.05),其中进行期患者组显著高于静止期(P<0.05),且血清sCD40L浓度变化与寻常性银屑病严重程度指数PASI有相关性(r=0.435,P<0.05)。结论检测血清sCD40L水平有助于判断寻常性银屑病病情的严重程度,MTX可通过降低血清sCD40L水平而起到治疗作用。     

天疱疮121例临床分析

目的：加强对天疱疮的认识,提高治疗水平。方法：回顾性分析121例天疱疮患者的临床资料。结果：大部分天疱疮患者通过激素及免疫抑制剂的治疗均能获得临床缓解,免疫抑制剂、血浆置换、大剂量丙种免疫球蛋白冲击治疗等＂辅助疗法＂能减少激素用量及其所产生的远期并发症,自体外周血造血干细胞移植有望根治难治性天疱疮。结论：目前天疱疮的治疗药物仍以激素为主,但由于其副作用,一些新型的治疗方案的研究正积极展开。