抑制 BHK21细胞 STYK1/NOK基因的转录组分析 #br#

目的 转录组分析抑制丝氨酸-苏氨酸-酪氨酸激酶 1（STYK1/NOK）后仓鼠肾正常细胞 BHK21的基因表 达,并分析其可能作用的信号通路。方法 BHK21细胞分为空载体组（转染 6 µg空载体 pBs/U6）、低转染浓度组（2 µg si-STYK1/NOK质粒+4 µg空载体 pBs/U6）和高转染浓度组（6 µg si-STYK1/NOK质粒）。转染 48 h后,Western blot 检测 STYK1/NOK蛋白表达,基于检测结果,选取空载体组和高转染浓度组提取总 RNA进行转录组测序。将测序所 得的序列进行过滤比对,获得差异表达基因后,应用 R软件进行生物功能（GO）分析和 KEGG信号通路分析。应用 STRING蛋白质互作数据库中的互作关系进行差异基因蛋白互作网络的分析。采用 qRT-PCR验证关键差异表达基 因亚甲基四氢叶酸脱氢酶 2（Mthfd2）、转录激活因子 5（Atf5）,ⅡA分泌型磷脂酶 A2（Pla2g2a）、6-磷酸果糖-2-激酶/ 果糖-2,6-双磷酸酶 3（Pfkfb3）的表达。CCK-8法检测空载体组和高转染浓度组细胞增殖情况。结果 Western blot 结果表明高转染浓度组明显抑制 STYK1/NOK蛋白表达。高转染浓度组与空载体组相比,共发现差异表达基因 44 个,包括 19个上调基因和 25个下调基因。GO富集分析发现差异表达基因主要影响生物调控、细胞进程、代谢调控、 细胞生物过程、细胞、细胞部分、胞外区、配体和催化活性。KEGG通路分析发现差异表达基因主要集中作用于免疫 系统、癌症、细胞周期、信号转导和氨基酸代谢等方面。qRT-PCR结果表明,高转染浓度组Mthfd2、Atf5的相对表达量 显著上调（P＜0.05）,Pfkfb3、Pla2g2a的相对表达量显著下调（P＜0.05）,与测序结果相一致。细胞增殖实验表明高转 染浓度组细胞增殖明显高于空载体组（P＜0.01）。结论 抑制 BHK21细胞 STYK1/NOK表达后,致使 BHK21细胞原 癌基因表达增强,抑癌基因表达减少,促进细胞增殖。     

Evaluation of adenovirus 19a as a novel vector for mucosal vaccination against influenza A viruses

Since preexisting immunity and enhanced infection rates in a clinical trial of an HIV vaccine have raised some concerns on adenovirus (Ad) serotype 5-based vaccines, we evaluated the subgroup D adenovirus serotype Ad19a for its suitability as novel viral vector vaccine against mucosal infections. In BALB/c mice, we compared the immunogenicity and efficacy of E1/E3-deleted Ad19a vectors encoding the influenza A virus (IAV)-derived antigens hemagglutinin (HA) and nucleoprotein (NP) to the most commonly used Ad5 vectors. The adenoviral vectors were applied intranasally and induced detectable antigen-specific T cell responses in the lung and in the spleen as well as robust antibody responses. A prior DNA immunization significantly improved the immunogenicity of both vectors and resulted in full protection against a lethal infection with a heterologous H3N2 virus. Nevertheless, the Ad5-based vectors were slightly superior in reducing viral replication in the lung which corresponded to higher NP-specific T cell responses measured in the lungs.     

The chronic hepatotoxicity assessment of the herbal formula Zishen Yutai pill

Zishen Yutai pill (ZYP) is an oriental herbal formula, while hepatotoxicity assessment of ZYP was rarely evaluated. Therefore, our aim is to re-evaluate its hepatotoxicity in both normal and carbon tetrachloride (CCl₄) induced chronic liver injury rats. In the normal model, two doses of ZYP (1.575 and 9.450 g kg⁻¹ d⁻¹; i.e. 1 × , 6 × clinical doses) were given orally to rats for 24 weeks. In the chronic liver injury model, 10% CCl₄ was administered to rats abdominally twice a week at a dose of 5 mL kg⁻¹ for 12 consecutive weeks. Administration time started from 4 weeks after the beginning of CCl₄ treatment. Toxicological parameters included mortality, body weight, food consumption, clinical signs, biochemical parameters, gross observation, organ weight, necropsy findings and histopathology were monitored. In the normal model, we found no any mortality or abnormality in clinical signs, relative liver weight, biochemical parameters and histopathology in ZYP treatment groups. In the chronic liver injury model, liver damage related parameter such as ALT was elevated at the high dose of ZYP treatment in contrast to the CCl₄-treated group (P < 0.01). In histopathological assessment, there were no significant difference between ZYP treatment groups and CCl₄-treated group. No observed adverse effect on livers were established for 9.450 g kg⁻¹ d⁻¹ ZYP in the normal rats and 9.450 g kg⁻¹ d⁻¹ ZYP in the injury rats.     

Epidemiology of HIV and sexually transmitted infections in China

As the most populous country in the world, China has been confronted with the huge challenges that HIV and sexually transmitted infections pose. The HIV infection rate among adults is lower than 0.1%, however, the estimated number of HIV infections sits around one million, according to the estimation of some experts. HIV infection is highly prevalent in some regional areas of China and the incidence rate of sexually transmitted infections has sharply increased since the 1980s. The number of HIV infections may reach 10 million if no effective measures are taken. This article aims to outline the profile of the epidemic in China as well as to elaborate on the contributing factors and the response of the government and community.     

无烟医院政策执行的障碍

目的了解无烟医院政策执行的障碍,为发展干预策略提供科学的背景资料。方法联合应用专题小组访谈和个人深入访谈对医务人员进行调查。结果无烟医院政策执行的障碍有:①倾向因素:医务人员吸烟严重,上班时间吸烟常见;医务人员对吸烟和被动吸烟的危害认识不清;对创建无烟医院没有足够的信心;②促成因素:医院执行政策的能力不足;③强化因素:吸烟是个人的生活权利;吸烟受社会风俗的影响;领导不重视控烟;担心劝烟会影响同事关系或医患关系。结论研究结果表明,倾向因素不是阻碍医院执行无烟政策的主要因素,强化和促成因素才是主要障碍。采取综合性的干预措施,营造良好的控烟氛围,促进社区参与,提高医院控烟能力,效果或许更理想。