犬蛔虫成虫抗原的促B细胞有丝分裂的活性

在体内用正常小鼠脾及人体外周血淋巴细胞就犬弓蛔虫抗原（ＴｃＡｇ）对淋巴细胞的作用进行了研究。成虫的ＴｃＡｇ在浓度为１～１２５μｇ／ｍｌ时能刺激小鼠脾细胞增殖。ＴｃＡｇ的应答细胞为Ｂ细胞，因为经抗免疫球蛋白抗体和补体处理或尼龙棉柱分离后，应答消失。此应答不是由脂多糖（ＬＰＳ）的污染引起的，因为ＴｃＡｇ能刺激作为ＬＰＳ的低应答者的Ｃ３Ｈ／ＨｅＪ脾细胞。ＴｃＡｇ不但刺激增殖反应，而且刺激多克隆ＩｇＧ和ＩｇＥ产生。ＴｃＡｇ还刺激巨噬细胞产生白细胞介素－１并刺激人的Ｂ细胞。上述结果还表明ＴｃＡｇ是一个有效的促Ｂ细胞有丝分裂原，其活性与感染犬弓蛔虫宿主的免疫功能的改变有关。     

Disseminated Penicillium marneffei infection in acquired immunodeficiency syndrome: a case report

Penicillium marneffei(P.marneffei) is a facultative intracellular pathogen and the only therrmally dimorphic fugus. This fungal intection is cormmonly found in Southeast Asian,     

细胞色素P450/环氧-二十碳三烯酸系统与心肌缺血/再灌注损伤

近年细胞色素P450(CYP450)及代谢产物环氧-二十碳三烯酸(EETs)在心肌缺血/再灌注损伤中的作用受到重视.该系统有可能通过对氧自由基生成、钙超载、白细胞激活、一氧化氮、ATP敏感性钾通道、丝裂原激活的蛋白激酶等多靶点影响心肌缺血/再灌注损伤的程度,表现为加重还是减轻损伤与机体状态有关.鉴于心肌缺血及再灌注激活CYP450/EETs系统,探讨影响CYP450/EETs作用的因素,尤其是确定其保护作用的特征及信号转导机制,将进一步阐明心肌缺血/再灌注损伤的发病机制,为防治该类疾病提出新的思路.本文仅就CYP450/EETs系统改变与心肌缺血/再灌注损伤关系做一综述.     

Therapeutic benefit of TH-engineered mesenchymal stem cells for Parkinson's disease

The present study was designed to assess the potential of marrow stromal cells (MSCs) to deliver therapeutic genes to the brain and result in biologically significant functional recovery. The tyrosine hydroxylase (TH) gene was transfected to MSCs with an adeno-associated virus (AAV) vector. MSCs expressing TH gene were transplanted into the striatum of Parkinson's disease (PD) rat. The asymmetric rotation of these models after apomorphine administration was detected every week after transplantation. Six weeks after grafting, animals were sacrificed. Some brains were sectioned to do TH immunohistochemistry. The others were used to detect the dopamine levels by high-performance liquid chromatograph and electrochemical detection (HPLC-ECD). The results showed that MSCs multiply rapidly and formed fibroblast colony-forming units in primary culture. The gene expression efficiency was about 75%. The rounds of asymmetric rotation after apomorphine administration decreased after TH-engineered MSCs were grafted. Histological examination showed that TH gene was expressed around the transplantation points. The dopamine level in the lesioned striatum of rats injected with TH-MSCs was significantly greater than that in rats treated with LacZ-MSCs (P < 0.05). All the data demonstrated that MSCs could readily be genetically engineered. Therefore, MSCs could be useful gene delivery vehicles of gene therapy for Parkinson's disease.     

激素在严重急性呼吸综合征治疗中的应用

严重急性呼吸综合征是一种由新型冠状病毒所致的新发现的急性呼吸道传染病,患者肺部的病变进展迅速,严重者可发生急性肺损伤乃至呼吸窘迫综合征。世界卫生组织将其命名为严重急性呼吸综合征(severe acute respiratory syndrome,SARS)。目前关于SARS治疗中激素的应用问题仍是SARS     

我国药品不良反应研究现状及其对策

目的：对我国药品不良反应(ADRs)研究现状进行分析,为深入开展ADRs研究提供基础信息。方法：以“药品不良反应、ADRs”为关键词在中国期刊全文数据库中进行文献检索,根据文献所用的方法学对其进行分类统计,以分析ADRs研究现状。结果：近10年来,有关ADRs研究呈逐年增加趋势,但文献仍以病例报道为主．缺乏高质量、深入的流行病学调研。结论：随着“国家ADR监测信息网”的建立与不断完善,在加速ADRs信息传递与交流的同时,对于临床用药频度高、用药量大的常规用药,在有病例报告支持的基础上,开展深入的流行病学调研。从而为临床医生及人们的用药决策提供最佳证据。     

Colorectal cancer screening for the natural population of Beijing with sequential fecal occult blood test: a multicenter study

Objective To assess the prevalence of colorectal cancer(CRC)in Beijing and the reliability or the sequential fecal occult blood test(SFOBT)for CRC screening.Methods Of the natural population(48 100 persons)in several Beijing communities,we screened 26 827 persone with age over 30 using the SFOBT screening program,Guaiacum Fecal Occult Blood Test(GFOBT),lmmuno Fecal Occult Blood Test(IFOBT),and colonoscopies.Results The screening rate of the population was 74%.The positive rate of SFOBT was 5.6%.The prevalence of CRC in the entire population of Beijing was therefore calculated to be 36.57/10^5.Of 12 CRC detected patients,4 cases were in stage DukesA (33.33%),7 cases in stage Dukes B(58.33%),only 1 case(8.34%)in stage Dukes C.Conclusions The prevalence of CRC in Beijing is one of the highest in China,Individuals at high risk for CRC or those over 50 years of age should be considered as primary candidates for screening,SFOBT screening is a cost-effective and reliable method for early detection of CRC.     

北京地区108例SARS患者临床特征、治疗效果及转归分析

目的　分析北京地区SARS的临床特征及转归。方法　分析 2 0 0 3年 3月 1 1日至 4月1 5日收治的 1 0 8例SARS患者的临床资料。结果　男性 35例 (32 4% ) ,女性 73例 (67 6 % )。年龄1 3～ 83(37± 9)岁。医务人员 31例 (2 8 7% )。 94例 (87 0 % )有明确接触史。 2 2例 (2 0 4% )并存其他内科疾病。主要临床表现为发热、咳嗽、胸痛、胸闷、头痛等。多脏器受累多见。潜伏期 2～ 1 4d。有隐性感染者。轻型 5例 (4 6 % ) ,普通型 43例 (39 8% ) ,重型 2 2例 (2 0 4% ) ,极重型 38例(35 2 % )。典型病程临床呈早期、进展期、极期、恢复期四期经过。发病早期白细胞总数、淋巴细胞总数和比值、前白蛋白、转铁蛋白明显下降 ,C反应蛋白、α酸性糖蛋白和α2 球蛋白明显升高 ,CD+ 3、CD+ 4、CD+ 8细胞下降。X线胸片早期见肺内单发或多发局灶性渗出改变 ,多位于肺下野 ;进展期磨玻璃影或实变影增大。高分辨率CT表现为单发或多发“棉花团”样磨玻璃影或实变影。低氧血症较多。在疾病的进展期、极期合理使用激素和早期使用无创机械通气可阻止病情进展。抗生素可预防继发感染。 4例 (3 7% )遗留肺间质纤维化。 94例好转恢复出院 ,1 4例死亡。中医认为SARS属于温病 ,中西医结合治疗能减轻中毒症状 ,有助于恢复期患者     

大剂量环磷酰胺冲击疗法配伍激素治疗狼疮性肾炎的临床疗效观察

目的:探讨大剂量环磷酰胺(CTX)冲击疗法配伍激素治疗狼疮性肾炎的临床疗效。方法:将26例狼疮性肾炎患者分为2组,治疗组应用大剂量CTX冲击,每次0.8~1.0g溶于100ml生理盐水中于30分钟内快速静点,每月1次,总量8~10g,同时加服强的松1mg(kg/d),6~8周后逐渐减至维持量,对照组使用强的松单药口服治疗,对照组使用强的松单药口服治疗,强的松用法及减量方法与上述相同。结果:两组多项观察指标比较具有显著性差异(P<0.05)。结论:大剂量环磷酰胺冲击疗法配伍强的松治疗狼疮性肾炎近期疗效优于单药强的松治疗。