北京地区成年人社区获得性肺炎中肺炎支原体急性感染的调查

肺炎支原体(Ⅷ,)是成年人社区获得性肺炎(CAP)中常见病原体,感染率达到20％一30％。为了解成年人MP急性感染肺炎的流行特点。由首都医科大学附属北京朝阳医院和北京市疾病预防控制中心牵头,组织北京地区9家二级和三级综合医院成立成年人肺炎监测网(BNACAP),以“恢复期较急性期斑清肺炎支原体IgG抗体≥4倍升高”作为MP急性感染的诊断标准,从2010年11月至2011年9月对入组的410例成年人CAP进行流行病学分析。     

Genetic diversity and zoonotic potential of human rotavirus strains, 2003–2006, hungary

Rotavirus strain surveillance is being conducted in many countries before and after introduction of newly licensed vaccines to assess the impact of the vaccines on rotavirus strains. Here we describe a strain surveillance study in the Budapest area of Hungary (2003–2006) based on RNA profile analysis, genotyping by multiplex PCR and nucleotide sequencing. Among 1,983 G‐typed rotaviruses we identified G1 (22%), G2 (4.8%), G3 (3.5%), G4 (18.5%), G6 (1.1%), G8 (<0.1%, n = 1), G9 (42%), and G12 (3.4%) specificities. Information on P genotype incidence was determined for a subset of samples (n = 814). In addition to the globally important strains, a variety of uncommon antigen combinations were also found, for example, P[9],G3; P[14],G6; or P[14],G8. Sequence and phylogenetic analysis of the VP7, VP4, VP6, and NSP4 genes of selected strains with uncommon antigen combinations demonstrated high similarity with certain bovine, porcine, feline, equine, and lapine rotaviruses, respectively. Continued surveillance is needed to assess the role of animal rotaviruses in human diseases. J. Med. Virol. 81:362–370, 2009. © 2008 Wiley‐Liss, Inc.