盐酸洛美沙星眼凝胶临床生物等效性试验

目的:观察3g/L盐酸洛美沙星眼凝胶的临床疗效及安全性。方法:对128例(203眼)细菌性结膜炎和细菌性角膜炎患者采用多中心、随机分组、平行对照试验,观察其主要症状体征消失情况、角膜炎症面积缩小情况、病原菌清除效果和不良反应。试验组(testgroup,TG)65例(103眼),对照组(controlledgroup,CG)63例100眼,分别应用3g/L盐酸洛美沙星眼凝胶和3g/L盐酸洛美沙星滴眼液。结果:①TG治愈率(80.6%)高于CG治愈率(65.0%);②TG(细菌性结膜炎)眼痛异物感消失率和消失时间(83.7%,5.33±2.00d)优于CG(64.1%,6.36±1.50d);TG(细菌性结膜炎)流泪消失率(91.8%)高于CG(75.0%);③记分统计表明:TG(细菌性结膜炎)结膜充血改善(平均下降1.76分)明显优于CG(平均下降1.25分);TG畏光消失情况(平均下降1.24分)好于CG(平均下降0.87分);TG(细菌性结膜炎)分泌物消失情况(平均下降1.64分)好于CG(平均下降1.33分);④其他情况两组间无明显差异。结论:3g/L盐酸洛美沙星眼凝胶治疗眼前段细菌感染安全、有效,对结膜炎症状体征的改善优于滴眼液。     

关节镜下同种异体髌腱组织重建膝前交叉韧带的初步临床研究

目的　探讨关节镜下应用异体髌腱 (骨 腱 骨组织 ,BPTB)重建膝关节前交叉韧带 (an teriorcruciateligament,ACL)的方法及初步临床疗效。 方法　关节镜下应用深低温冷冻异体BPTB移植重建ACL 8例。对手术时间、手术切口及术后体温恢复、ACL体征、X ray检查分析手术疗效 ;术后关节屈曲度及Lysholm Ⅱ评分评定膝关节功能。结果　关节镜下应用异体BPTB重建ACL切口小 ,手术时间短 ,术后 5d内体温恢复正常 ,无一例感染及免疫排斥现象。平均随访时间 4个月 (2～ 6个月 ) ,所有患者症状均有缓解 ,前抽屉试验 ( ) (anteriordrawertest,ADT) ,Lachman( ) ,X ray检查界面螺钉在位 ,未见骨道异常 ;术后 2周平均屈曲度可至 10 5°(80°～ 130°) ,术后 2个月膝关节平均屈曲度可达 12 0°(10 0°～ 130°) ,术前Lysholm Ⅱ评分平均 38 5分 (2 4～ 5 0分 ) ,术后 2个月Lysholm Ⅱ评分平均 88分 (76～ 97分 )。结论　应用深低温冷冻异体髌腱重建ACL可减少手术创伤 ,早期即可有效改善膝关节功能 ,是一种可行的重建ACL的方法 ,远期疗效尚需进一步的观察。     

京剧各行当电声门图与嗓音声学检测参数分析与研究

目的探讨我国京剧艺术不同行当的电声门图和声测试的参数特点。方法采用电脑多媒体操作系统，使用Dr．Speech3．0软件，对137名20～64岁不同行当的专业演员发稳态元音屈／ae、／a：／、i：／同步进行声门图和声学测试的检测和分析，并以嗓音健康的普通人群作对照组。结果京剧艺术各行当元音／ae／、／a：／、／i：／声门图和声学测试除Fo差异比较显著外，其他声门图参数Fo、Jitter、Shimmer、NEE、CQ、CQP及声学测试参数Fo、Jitter、Shimmer、NEE均有其不同的参数值，有统计学意义（P〉0．01、P〉0．05）。对照组的各嗓音参数值均在DPSS提供的正常范围内。结论京剧各行当的嗓音有其独特的声学特征，引用Dr．Speech3．0软件可对各行当嗓音参数值进行临床客观检测与评估，对京剧嗓音的训练与保健提供了有益的临床价值。     

甘精胰岛素联合口服降糖药治疗2型糖尿病磺脲类继发性失效临床观察

目的：探讨甘精胰岛素联合口服降糖药物（拜糖苹或二甲双胍）治疗继发性磺脲类药物失效的2型糖尿病的短期疗效。方法：46例继发性磺脲类药物失效的2型糖尿病患者随机分成两组，A组使用甘精胰岛素加拜糖苹或二甲双胍，B组使用中效胰岛素加拜糖苹或二甲双胍。所有患者均于治疗前、治疗后12周测定空腹血糖（FBG）、餐后2h血糖（2hBG）、糖化血红蛋白（HbAte）。结果：两组治疗3个月后，FBG、2hBG、HbA。。均较治疗前明显降低，对于空腹血糖的控制A组明显优于B组，有统计学意义（P＜0．05），且A组低血糖发生率明显低于B组（P＜0．05）。结论：甘精胰岛素联合口服降糖药治疗继发性磺脲类药物失效的2型糖尿病患者，可以良好地控制高血糖，且低血糖发生率低，安全而有效。     

多级图像对比增益(MUSICA)在全脊柱摄影后处理中的应用探讨

目的：探讨用计算机摄影（Computer radiography）进行全脊柱摄影时，运用多级图像对比增益（MUSICA）软件后处理技术的临床价值。方法：应用全脊柱专用暗盒架（2～3个IP板）一次曝光，分别逐个采集IP板信息至图像后处理工作站。经全脊柱模式对所选多幅图像执行自动化图像拼合，生成一幅完整全脊柱片。结果：2500例全脊柱图像明显得到优化。结论：多级图像对比增益（MUSICA）的应用，为临床科医生测量脊柱（COBB角）的精确度、制定术前方案、术后疗效提供了更多的信息资料。     

复方奥硝唑凝胶的研制与质量控制

目的:制备复方奥硝唑凝胶,并进行质量控制。方法:以0．6％卡波姆-940为基质,奥硝唑和西咪替丁为主药制备凝胶;采 用紫外分光光度法直接测定2组分含量,并考察成品稳定性。结果:所得凝胶外观、成形及涂展性良好;奥硝唑、西咪替丁的检测浓 度线性范围均为0．5-32μg／ml,平均回收率分别为100．58％、100．59％,相对标准差分别为1．17％、1．61％;稳定性考察结果符合 《中国药典》2000年版的规定。结论:复方奥硝唑凝胶配方合理,制备工艺简单,质量可靠,性质稳定。     

Alopecurone B逆转人乳腺癌细胞MCF-7阿霉素耐药株活性研究

目的 研究黄酮化合物Alopecurone B（APB）对人乳腺癌细胞阿霉素耐药株MCF-7/ADR多药耐药的逆转活性及逆转机制。方法 人乳腺癌细胞阿霉素耐药株MCF-7/ADR维持在含有250 ng/mL阿霉素的培养基中,使用MTT法、qPCR、Western blot和流式细胞术研究APB对耐药株P-糖蛋白(P-gp)功能和表达的影响。结果 APB能逆转MCF-7/ADR细胞多药耐药,抑制P-gp的功能,下调P-gp基因和蛋白的表达。结论 APB具有强效逆转人乳腺癌细胞MCF-7/ADR多药耐药的效果,其机制涉及对P-gp的调控。      

冠脉CTA在肥胖患者中的应用:APSCM与100 kVp管电压的比较

  目的  在自动管电压选择（automated tube voltage selection，APSCM）与定向100 KVp管电压两种情况下，比较超重患者冠状动脉计算机体层血管造影（coronary computed tomography angiography，CCTA）的图像质量和辐射剂量。  方法  选取115位体重指数（BMI）在25～30 kg / m2 之间，并且体重低于90 kg的患者在第二代双源CT扫描仪上进行CCTA检查，所有检查均采用前瞻性心电门控及大螺距（3.4）模式。所有患者被随机分为A、B两组:A组75例，采用APSCM技术进行检查；B组40例，接受固定100 kVp管电压检查。测量CT衰减和图像噪声，并计算信噪比（signal-to-noise ratio，SNR）和对比噪声比（contrast-to-noise ratio，CNR）。对两组图像质量及辐射剂量进行比较。  结果  A组中，1例（1.3%）、51例（68%）和23例（30.7%）患者的管电压自动选择为80 kVp、100 kVp、120 kVp，平均CT衰减和图像噪声显著低于B组（P < 0.001）。A组的平均CNR高于B组，平均SNR较低（P < 0.05）。两组的主观图像质量得分无显著差异（P > 0.05），B组的辐射剂量比A组低22.2%（P = 0.004）。  结论  对于BMI高于25 kg / m2、低于30 kg / m2，且体重低于90 kg的患者，与使用APSCM的CCTA相比，100 kVp CCTA可以降低辐射剂量而不影响图像质量。     

Interleukin-34 accelerates intrauterine adhesions progress related to CX3CR1+ monocytes/macrophages

Intrauterine adhesions (IUA) are characterized by endometrial fibrosis and impose a great challenge for female reproduction. IL-34 is profoundly involved in various fibrotic diseases through regulating the survival, proliferation, and differentiation of monocytes/macrophages. However, it remains unclear how IL-34 regulates monocytes/macrophages in context of IUA. Here, we showed that the expression level of IL-34 and the amount of CX3CR1+ monocytes/macrophages were significantly increased in endometrial tissues of IUA patients. IL-34 promoted the differentiation of monocytes/macrophages, which express CX3CR1 via CSF-1R/P13K/Akt pathway in vitro. Moreover, IL-34-induced CX3CR1+ monocytes/macrophages promoted the differentiation of endometrial stromal cells into myofibroblasts. Of note, IL-34 caused endometrial fibrosis and increased the amount of CX3CR1+ monocytes/macrophages in endometrial tissues in vivo. IL-34 modulated endometrial fibrosis by regulating monocytes/macrophages since the elimination of endometrial monocytes/macrophages significantly suppressed the profibrotic function of IL-34. Finally, blocking of IL-34 in the LPS-IUA model resulted in the improvement of endometrial fibrosis and decreased number of CX3CR1+ monocytes/macrophages. Our studies uncover the novel mechanism of interaction between IL-34-induced CX3CR1+ monocytes/macrophages and endometrial stromal cells in endometrial fibrosis pathogenesis, and highlight IL-34 as a critical target for treating IUA.     

Local bone quality measurements correlates with maximum screw torque at the femoral diaphysis

BackgroundSuccessful fracture fixation depends critically on the stability of the screw-bone interface. Maximum achievable screw torque reflects the competence of this interface, but it cannot be quantified prior to screw stripping. Typically, the surgeon relies on the patients' bone mineral density and radiographs, along with experience and tactile feedback to assess whether sufficient compression can be generated by the screw and bone. However, the local bone quality would also critically influence the strength of the bone-screw interface. We investigated whether Reference Point Indentation can provide quantitative local bone quality measures that can inform subsequent screw-bone competence.MethodsWe examined the associations between the maximum screw torque that can be achieved using 3.5 mm, 4.5 mm, and 6.5 mm diameter stainless steel screws at the distal femoral metaphysis and mid-diaphysis from 20 cadavers, with the femoral neck bone mineral density and the local measures of bone quality using Reference Point Indentation.FindingsIndentation Distance Increase, a measure of bone's resistance to microfracture, correlated with the maximum screw stripping torque for the 3.5 mm (p < 0.01; R = 0.56) and 4.5 mm diameter stainless steel screws (p < 0.01; R = 0.57) at the femoral diaphysis. At the femoral metaphysis, femoral neck bone mineral density significantly correlated with the maximum screw stripping torque achieved by the 3.5 mm (p < 0.01; R = 0.61), 4.5 mm (p < 0.01; R = 0.51), and 6.5 mm diameter stainless steel screws (p < 0.01; R = 0.56).InterpretationReference Point Indentation can provide localized measurements of bone quality that may better inform surgeons of the competence of the bone-implant interface and improve effectiveness of fixation strategies particularly in patients with compromised bone quality.