藻酸钙在鼻中隔手术中的应用

目的 观察藻酸钙与凡士林纱条在鼻中隔手术中应用的效果.方法 60例鼻中隔偏曲在本科接受手术治疗的成年患者,随机分组,实验组和对照组各30例.两组患者接受相同的手术治疗(鼻中隔黏膜下切除术)及常规处理.实验组采用藻酸钙(Sorbalgon)、凡士林纱条鼻腔填塞;对照组采用常规的凡士林纱条填塞.结果 实验组患者鼻腔填塞后自觉不适反应轻;鼻腔尤其鼻中隔黏膜损伤反应较对照组轻,创面渗出少,鼻中隔黏膜修复优于对照组.结论 藻酸钙具有良好的保护创面作用,有利于鼻腔黏膜的早期愈合.     

VCP蛋白表达与胃癌预后的相关性研究

目的：探讨VCP蛋白表达与胃癌预后的关系，确立VCP作为判断胃癌预后的独立因子作用。方法：在187倒胃癌组织手术标本中进行VCP的免疫组织化学染色，比较肿瘤细胞和正常血管内皮细胞VCP的表达，并结合肿瘤浸润、转移、预后等临床因素进行分析比较。结果：VCP的表达与胃癌的浸润、淋巴结转移相关，单变量分析中年龄、胃癌的浸润深度、脉管浸润、淋巴结转移及远处转移等因素显示与胃癌的预后相关（P〈0．05），多变量分析显示了VCP表达水平较肿瘤的分化、淋巴结浸润、转移与胃癌预后相关的危险系数高，故VCP的表达更能反映胃癌的预后。结论：VCP的表达是胃癌重要、独立的预后因子。     

羊水性状及脐动脉S/D与新生儿Apgar评分关系的探讨

目的分析羊水指数、脐动脉S/D和新生儿Apgar评分资料,探讨其相互关系。方法37周以上、在分娩前3d内接受了超声检查的孕妇263例为研究对象。年龄（30.37±3.27）岁,孕龄（39.24±1.25）周;排除胎儿畸形、中度以上妊娠高血压综合征、严重孕妇疾病等异常情况。四象限法测量羊水指数,根据内部回声情况对羊水性状进行评价;频谱多普勒超声测量脐带动脉SV、DV和S/D;胎儿出生后进行Apgar 1min评分。统计学相关分析。结果羊水指数和羊水性状与胎儿脐带动脉S/D显示了一致的变化;以胎儿脐带动脉S/D≥3.0,Apgar 1 min评分≤7有22例,二者呈显著相关（R=0.667,P=0.000）;羊水指数和羊水性状与新生儿Apgar 1 min评分也显示了良好的相关性（R分别为0.667,0.513和0.765,P=0.000）。结论羊水指数和羊水性状与胎儿脐带动脉S/D对评价新生儿缺氧有相同的意义。     

2型糖尿病大鼠氧化应激介导肝脏和骨骼肌细胞内脂肪堆积的差异

目的观察2型糖尿病大鼠高脂饮食后肝脏和骨骼肌氧化应激及脂质堆积的差异。方法10只雄性Goto Kakisaki（GK）大鼠随机分为两组：糖尿病对照组和糖尿病硫辛酸治疗组（α-硫辛酸35mg／kg隔天腹腔内注射1次），健康Wistar大鼠4只为正常对照组，高脂饮食12周。测定各组大鼠肝、骨骼肌匀浆中的GSH、SOD和MDA及脂质谱水平，以及观察其组织病理形态学改变。结果糖尿病对照组肝组织GSH、SOD和MDA及脂质谱水平较正常对照组明显异常，经α-硫辛酸治疗后有明显改善，且GSH、SOD和MDA水平与甘油三酯水平相关；骨骼肌组织中除SOD外，GSH和MDA以及脂质谱水平在3组之间的变化，差异无统计学意义（P〉0．05）。糖尿病对照组有明显的肝细胞脂肪变性、坏死及炎性细胞浸润，硫辛酸治疗后有明显改善，而骨骼肌组织病理形态学无明显变化。结论12周高脂饮食可导致明显的氧化应激状态和细胞内脂肪堆积，但肝脏和骨骼肌两者之间存在一定差异，α-硫辛酸通过改善氧化应激状态而对细胞脂质代谢异常有明显的缓解作用。     

AO技术治疗Pilon骨折(附46例报告)

目的：评价AO技术治疗Pilon骨折的临床疗效。方法：自1995年7月～2002年6月应用AO技术对46例Pilon骨折行切开复位，支撑钢板及螺钉内固定治疗。术后随访0．5-7年，按Mazur标准进行功能评价。结果：46例患者总优良率84％，其中C1型20例，O2型15例，Q型4例。7例发生切口感染，8例出现创伤性踝关节炎。结论：临床治疗效果与骨折类型及治疗方法有关，运用AO技术治疗Pilon骨折可以取得满意的疗效。     

Novel SLA-DQ alleles and their recombinant molecules in xenogeneic stimulation of human T cells

MHC class II antigens DR and DQ are essential for graft rejection both in allo- and xeno-transplantation. The antigens, especially the DQA and DQB gene-coencoded DQ molecules, are also involved in transplantation tolerance induced by activation of regulatory T cells. Here we report six novel DQ alleles from three properly inbred Chinese pig strains Gz, Bm and Yn. In our study, cDNA of swine leukocyte antigen (SLA)-DQA and -DQB were amplified by RT-PCR and sequenced for each strain. The ORF-containing SLA-DQA and -DQB genes are composed of 768 (or 765) and 786 nucleotides, encoding antigen molecules of 255 (or 254) and 261 amino acid residues, respectively. Sequences of both SLA-DQA and -DQB alleles showed disparities when compared either among the three pig strains or with available SLA data, which allows our novel alleles receiving their accession numbers from GenBank. The sequence analysis further revealed a phylogenic connection of our SLA-DQ alleles with SLA-DQ(c) haplotype. In addition, the homologies of MHC DQ or DQ-like molecules between Chinese pigs (SLA) and human (HLA) are higher than those between pigs and mice (H-2). By co-transfection of Bm pig DQA and DQB genes into L929 cells, the Bm-DQ heterodimer-expressed cells could effectively stimulate the human lymphoproliferation in presence of human APCs with a mean stimulation index (SI) 9.9+/-1.4. This functional assay indicated that our recombinant DQ antigens are capable of initiating human lymphoproliferation in a xeno-MLR.     

论植入性医疗器械临床流通规范化全过程管理

如何对植入性医疗器械临床流通过程进行规范管理一直是困绕医院管理者的话题。我院通过开展全过程医疗质量实时监控,规范了植入性医疗器械从购销、使用、随访整个临床流通过程的管理,充分保障了患者用械安全。     

滋阴潜阳法治疗老年高血压病临床观察

目的观察滋阴潜阳法治疗阴虚阳亢型老年高血压病的疗效。方法将120例辨证为阴虚阳亢证的老年高血压病患者随机分为治疗组(中药加氨氯地平)60例与对照组(氨氯地平)60例,治疗8周;比较两组治疗前后临床症状、24h平均血压、血脂的变化和降压疗效。结果治疗组疗效优于对照组,在改善血脂方面亦优于对照组。结论滋阴潜阳法治疗阴虚阳亢型老年高血压病疗效确切。     

Clinical survey of neurosensory side-effects of mandibular parasymphyseal bone harvesting

The aim of the present survey was to assess neurosensory disturbances and/or tooth-pulp sensitivity losses after mandibular parasymphyseal bone-harvesting procedures. Twenty-eight harvesting areas in 16 patients were surveyed. Mucosal and skin sensitivity of the chin/lower lip, divided into four regions, were determined via Pointed-Blunt and Two-Point-Discrimination Tests. Pulp sensitivity of the mandibular teeth from the left second bicuspid to the right second bicuspid was tested by cold vitality preoperatively and 12 months postoperatively. Teeth were grouped according to sensitivity alterations and distance from the harvesting defects, as measured on CT scans, and statistically significant differences sought. At 12 months, 29% of preoperatively vital cuspids overlying the harvesting defects revealed pulp-sensitivity losses; no patient reported anaesthesia or analgesia; hypoaesthesia was present in 4% (8 sites; 2 patients), hypoalgesia was present in 3% (5 sites; 2 patients) and Two-Point-Discrimination Tests yielded pathologic responses in 5% of tested areas (10 sites; 4 patients). Teeth with and without pulp sensitivity changes were statistically indistinguishable regarding distances between root apices or mental foramen and the harvesting defect. The loss of pulp sensitivity in any tooth cannot be predicted simply on the basis of the distance between its apex and the harvesting osteotomy line.