Effects of DNMT3A on mouse cardiac fibroblast proliferation and migration under high glucose environment北大核心CSCD

Aim To investigate the effect of DNA methyltransferase 3A (DNMT3A) on the proliferation and migration of cardiac fibroblasts (CFs) in C57 mice under high glucose environment. Methods The hearts of C57 mice were taken from 1 to 3 days. After cutting and digesting, CFs were extracted by differential adherance centrifugattion and observed under microscope. After cell attachment, the cells were cultured under low glucose (5.5 mmol • L -1) medium and high glucose (33.0 mmol • L-1) medium and treated using DNMT3A lentivirus ( to silence the DNMT3A gene) . qRT-PCR was used to detect mRNA expression of DNMT3A, a-smooth muscle actin ( α-SMA ) and type I collagen procollagen Al ( Coll Al ) , the protein expression of DNMT3A, α-SMA and CollAl was assayed by Western blot, cell proliferation activity was detected by CCK-8 assay and MTT assay,cell migration ability was detected by scratch assay and Transwell experiment. Results Compared with the low glucose group,the high glucose environment resulted in up-regulated DNMT3A expression in CFs, increased collagen fiber deposition, and promoted CFS proliferation and migration. The expression of DNMT3A was down-regulated in CFs treated with DNMT3A lentivirus under high glucose condition, α-SMA, CollAl expression was down regulated, and cell proliferation and migration ability decreased. Conclusions Silencing of DN-MT3A could inhibit the proliferation and migration of CFs under high glucose environment, suggesting that DNMT3A may be a key regulatory point in the develop¬ment of diabetic cardiomyopathy. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

开胸术中保护性肺通气策略研究进展

术后发生肺损伤严重影响患者预后,meta分析发现术后发生肺损伤的患者滞留ICU由1 d延长到8 d,病死率增加19.00%,腹部手术为12.20%,而开胸术后可达26.50%.[1].术后肺损伤的发生不仅与患者术前肺部情况、手术操作及术中输血输液有关,也与术中通气模式有关.保护性肺通气(protective lung ventilation,PLV)越来越受到关注,本文就开胸术中PLV进行综述.     

不同预充气量及自动调节充气量时置入Supreme喉罩难易比较

目的比较不同预充气量及自动调节充气量时的Supreme喉罩（SLMA）置入难易程度。方法 180例ASAⅠ或Ⅱ级妇科、乳腺、四肢及胆囊择期手术患者全麻下置入4号SLMA,按通气罩预充气量不同及自动调节充气量（简称自动）时,采用随机数字表法均分为0 ml组（A组）、5 ml组（B组）、10 ml组（C组）、15 ml组（D组）、20 ml组（E组）、自动组（F组）,记录SLMA置入次数、置入时间及难易程度评分。结果在多组间比较中,置入评分为1分的患者C组和F组多于A及E组（P〈0.05）;4分的患者C组和F组小于A及E组（P〈0.05）;3分的F组明显少于其他组（P〈0.05）。置入时间C组和F组明显少于其他组（P〈0.05）。结论 SLMA预充气量为10 ml时及自动调节充气量时置入时间更短,置入更容易。     

不同剂量1.5%氯普鲁卡因缓解分娩镇痛爆发痛的效果

目的 探讨1.5%氯普鲁卡因硬膜外注射量缓解分娩镇痛中爆发痛的效果。方法选择硬膜外麻醉分娩镇痛后出现爆发痛的初产妇128例，随机分为R组、C₁组、C₂组和C₃组。出现爆发痛时，R组硬膜外注射0.15%罗哌卡因6 mL,C₁组、C₂组、C₃组硬膜外分别注射1.5%氯普鲁卡因6、8、10 m L。给药后每隔3 min对产妇进行1次疼痛视觉模拟评分(VAS)评估镇痛效果。记录主要观察指标：爆发痛给药起效时间和爆发痛治疗的成功率。次要观察指标：补救镇痛率；产妇满意度(爆发痛给药后5 min、胎儿娩出后24 h)；首次爆发痛发生时(T₀)、给药后3 min(T₁)、6 min(T₂)、9min(T₃)、12 min(T₄)、15 min(T₅)、18 min(T₆)的疼痛VAS、改良Bromage评分、胎心率(FH...