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A novel surgical technique to prevent post-enucleation conjunctival cyst:conjunctival staining with methylthioninium 下载免费PDF全文
Dear Editor,Conjunctival cyst of the orbit acquired from enucleation is an infrequent but serious complication;which can occur in 3%-7%of patients,more commonly after a secondary procedure;.The most common manifestations are the inability to retain the external prosthesis. 相似文献
3.
《Vaccine》2016,34(50):6316-6322
ObjectiveAir pollution, weather condition and influenza are known risk factors of acute coronary syndrome (ACS) among elderly people. The influenza vaccine (IV) has been shown to reduce major cardiovascular events. The purpose of this study was to compare resistance to air pollution and weather factors causing ACS between vaccinated and less-vaccinated elderly people.MethodsA case–crossover design was applied to 1835 elderly ACS patients who were obtained from the 1-million sample of Taiwan National Health Insurance Research Data with inclusion criteria: (1) the first diagnosis of ACS was in cold season and at age 68 or more, (2) had received the free IV program at least once during the period 3 years before the ACS. They were stratified into two groups: 707 had received flu vaccinations for all the 3 years and the remaining 1128 had not. The measurements of air pollutants, temperature, and humidity corresponding to each of the 3 days prior to the ACS diagnosis date were retrieved from the data banks of the Taiwan Environmental Protection Administration and Central Weather Bureau.FindingsIncreases in air pollution concentrations of CO, NO2, PM10 or PM2.5 and decreases in temperature significantly influenced the risk of ACS for the non-continuously vaccinated elderly population; however, less significant effects were observed for the continuously vaccinated population.ConclusionConsecutive influenza vaccination may potentially offer resistance against the detrimental effects of air pollution and changes in temperature in frail elderly adults with ACS. Future studies are needed to directly assess the interaction effect between the vaccination and environmental factors on ACS. 相似文献
4.
Bi-Hua BIE Yong CHEN Zhi-Qi ZHAO Institute of Neurobiology Fu-Dan University Shanghai China Institute of Shanghai Physiology Chinese Academy of Sciences Shanghai China 《中国神经科学杂志》2006,(2)
Objective Ligustrazine, also named as tetramethylpyrazine, is a compound purified from Ligusticum chuanxiong hort and has ever been testified to be a calcium antagonist. The present investigation was to determine the antinoci-ceptive effect of ligustrazine and, if any, the peripheral ionic mechanism involved. Methods Paw withdrawal Latency ( PWL) to noxious heating was measured in vivo and whole-cell patch recording was performed on small dorsal root ganglion (DRG) neurons. Results Intraplantar injection of ligustrazine (0.5 mg in 25μl) significantly prolonged the withdrawal latency of ipsilateral hindpaw to noxious heating in the rat. Ligustrazine not only reversibly inhibited high-voltage gated calcium current of dorsal root ganglion (DRG) neuron in dose-dependent manner with IC50 of 1.89 mmol/L, but also decreased tetrodotoxin (TTX) -resistant sodium current in relatively selective and dose-dependent manner with IC50 of 2.49 mmol/L. Conclusion The results suggested that ligustrazine could elevate the threshold of thermal nociception through inhibiting the high-voltage gated calcium current and TTX-resistant sodium current of DRG neuron in the rat. 相似文献
5.
维甲酸硅油的视网膜毒性实验研究 总被引:1,自引:0,他引:1
目的:了解维甲酸硅油对视网膜是否产生毒性。
方法:12只新西兰白兔的24只眼,随机分为3组,行气体压迫玻璃体手术3天后,分别向玻璃体腔内注入硅油(4只眼)、5μg/ml维甲酸硅油(10只眼)、10μg/ml维甲酸硅油(10只眼)各
0.5m1,用检眼镜、光漳和电镜检查来观察视网膜变化情况。
结果:注入硅油28天后,未发现各浓度的维甲酸硅油对视网膜产生毒性作用。结论:浓度为5、10μg/ml的维甲酸硅油注入玻璃体腔4周,对视网膜不产生毒性作用。
(中华眼底病杂志,1997,13:81-82) 相似文献
6.
Shi-Feng Li Yu-Guang Chen Yuan-Chang Yan Yi-Ping LiInstitute of Biochemistry Cell Biology Shanghai Institute of Biological Sciences Chinese Academy of Sciences Shanghai ChinaCollege ofLife Science Shanghai University Shanghai China 《Asian journal of andrology》2004,(4)
Aim: To study the characteristics and possible retention function of specific sequence in the 5'-end of rat testis GABAA receptor β 3t variant. Methods: Rat testis GABAA receptor β 3t variant cDNA was cloned and inserted into two eukaryotic expression vectors of pEGFP-Nl and pEGFP-Cl respectively, which have EGFP reporter gene. The recombinant plasmids were transfected into Chinese hamster ovary (CHO) cells with the calcium phosphate co-precipitation method. Fluorescence microscope and laser confocal microscope were used to analyze the transfected cells. ConA-Texas-Red was used to label cell endoplasmic reticulum (ER) and study the located area of rat testis β 3t variant in the CHO cells. Results: When the rat testis β 3t variant express in CHO cells, there were two expression pattern, the even and the concentrated expression pattern. Although rat brain β 3 can reach cell membrane, the rat testis β 3t variant can't target to the cell membrane. It may be retained in the ER of CHO cells. Conclusion: 相似文献
7.
免水分离超声乳化白内障摘除术临床观察 总被引:2,自引:0,他引:2
目的 :探讨免水分离术在超声乳化白内障摘除术中临床应用的有效性。方法 :随机选择白内障患者 2 0 0只眼 ,分两组。一组 (对照组 ) 95例 10 0只眼进行常规水分离术 ,二组 (观察组 ) 94例 10 0只眼 ,行免水分离术 ,所有患者均由同一医师进行超声乳化白内障摘除术联合人工晶体植入。临床观察超声乳化能量和时间 ,术后第一天、第三天、一周时视力 ,术中、术后并发症。结果 :( 1)平均超声能量Ⅰ、Ⅱ、Ⅲ、Ⅳ级核 ,一组分别是 :( 10 5 6± 4 16) %、 ( 17 88± 2 2 6) %、 ( 2 9 2 2± 3 5 4) %、 ( 3 7 88± 2 67) % ;二组分别是 ( 11 3 0± 1 89) %、 ( 18 76± 1 61) %、 ( 2 7 99± 3 5 8) %、 ( 3 9 2 2± 3 3 3 ) % ;两组平均超声能量差异无显著意义 (t =-0 5 12、 -1 496、 1 70 6、 -1 3 60 ,P =0 615、 0 14 2、 0 0 91、 0 182 ) ;平均超声时间一组分别为 ( 7 5 6±3 2 1)s、 ( 2 6 68± 5 15 )s、 ( 71 72± 17 2 2 )s、 ( 13 7 71± 3 8 60 )s ,二组分别为 ( 9 10± 1 2 0 )s、 ( 2 4 71± 4 0 9)s、 ( 76 17± 18 74)s、 ( 12 2 0 9± 3 5 14 )s ,两组平均超声时间差异无显著意义 (t =-1 42、 -1 415、 -1 3 69、 1 3 3 3 ,P =0 173、0 164、 0 174、 0 190 )。 ( 2 )术后一 相似文献
8.
Novel multi-probe RNase protection assay set for detection of endotoxin associated receptors gene expression 总被引:2,自引:0,他引:2
Objective: To construct the multi-probe ribonuclease protection assay (RPA) template set to be used for detecting expression patterns of MD-2, TLR4, CD14 mRNAs in human peripheral blood mononuclear cells. Methods : The designed cDNA fragments of the three genes were generated by polymerase chain reaction (PCR)using specific primers and directionally cloned into EcoR I and Hind III sites of expression plasmid pSP72 containing the T7/ promoter, the linearized plasmids was used as template to synthesize anti-sense RNA probes. Then we extracted total RNA from peripheral blood mononuclear cells (PBMC) and detected the dynamic expression patterns of the three genes with RPA method. Results: The proper sequence and orientation of the template set were confirmed by sequencing and the template set was successfully used to assay TLR4, MD-2 and CD14 mRNAs in human PBMC. The results showed that the three detected genes decreased transiently 1-3 hours after 100 ng/ml LPS stimulation. Conclusions: These new RPA multi-probe set provided valuable tool for the simultaneous quantitative determination of expression of TLR4, CD14 and MD-2 mRNAs in both constitutive and inducible types. 相似文献
9.
杨云霞 《中华医学美学美容杂志》2007,13(4):211-213
目的在应用“横切法”内眦开大的基础上,探寻一种充分开大内眦,切口瘢痕隐蔽,术后内眦赘皮不易复发,效果持久的手术方法。方法水平切开皮肤赘皮及部分错位的眼轮匝肌纤维,将内眦点向内侧移位,并与鼻侧腱膜固定,保留内眦点上方赘皮皱褶,剪除少许眼轮匝肌及退缩于该处的眼轮匝肌纤维束,将皮肤皱褶下充分松解并使该处形成自然凹陷后,与重睑线顺延缝合。结果本组260例中,190例得到1个月至2年的随访,内眦赘皮矫正充分,形态满意者156例,内眦赘皮部分矫正,形态改善者19例,术后赘皮复发者8例,严重瘢痕增生,影响内眦形态者7例。结论本方法内眦开大充分,切口瘢痕隐蔽,无需切除皮肤,赘皮不易复发,是矫正内眦赘皮简单而有效的方法。 相似文献
10.
我国丙型和戊型肝炎人群流行病学调查及流行因素的研究 总被引:55,自引:1,他引:54
为阐明丙型和戊型肝炎在我国的流行严重度和流行规律,采用描述流行病学、血清流行病学和分子流行病学相结合的研究方法,对两型肝炎的流行特征和流行因素进行了研究。结果发现;一般人群调查近9万人丙型肝炎和戊型肝炎的流行率各为2.2%和9.7%,散 生病毒性肝炎感染比率各为2.15%和16.4%。丙型肝炎在我国主要经血传播,与血液接触密切人群中HCV感染率高达50-70%,慢性化比比例高达40-60%,目前供 相似文献