彭氏枸杞枣仁饮治疗顽固性失眠62例临床观察

[目的]探讨彭氏枸杞枣仁饮治疗顽固性失眠的疗效。[方法]随机对照的方法,以枸杞子、炒枣仁、五味子三味以药代茶,疗程2周。[结果]总有效率治疗组91.93%,对照组56.67%。[结论]枸杞枣仁饮可明显改善顽固性失眠患者的睡眠质量,优于安定对照组。     

毛细管电泳法快速检测金黄色葡萄球菌

目的建立一种利用毛细管电泳(CE)从混合细菌标本中快速、选择性检测金黄色葡萄球菌的方法,以适应快速病原学诊断的要求。方法在电泳缓冲液中加入抗金黄色葡萄球菌特异性抗体包被的乳胶粒子,改变金黄色葡萄球菌的电迁移性质,达到目的菌与其他细菌分离的目的。结果该方法能在20 m in内从大肠埃希菌、铜绿假单胞菌和肺炎克雷伯菌的混合物中分离出金黄色葡萄球菌;通过对加入金黄色葡萄球菌的健康志愿受试者粪便标本不同时间流出组分的培养证实了方法的可靠性。结论抗体包被胶乳结合CE检测方法是一种快速的金黄色葡萄球菌检测技术,有进一步开发利用的价值。     

氢化可的松不同治疗方案对脓毒性休克患者的影响

目的 探讨氢化可的松不同治疗方案对脓毒性休克患者的影响。方法 选取2014年6月~2015年6月于笔者医院重症医学科进行治疗的80例脓毒性休克患者,随机分为对照组和观察组,各40例。所有患者给予常规治疗,在此基础上,对照组单次缓慢静脉滴注氢化可的松,观察组持续静脉微量泵入氢化可的松。比较两组患者治疗前及治疗6、24、48h及5天时的血流动力学及动脉血乳酸变化,监测两组患者动态血糖,统计高血糖时间窗,比较两组患者预后情况。结果 在各时间点,观察组患者心率（HR）、平均动脉压（MAP）、中心静脉压（CVP）、及乳酸清除率（LCR）与对照组比较,差异均无统计学意义（P>0.05）;观察组患者平均血糖水平（MBG）与对照组比较,差异无统计学意义（P>0.05）,而最大血糖波动幅度（LAGE）、血糖波动系数（GV）及高血糖时间窗均明显低于对照组,差异具有统计学意义（P<0.05）;观察组患者休克持续时间、ICU住院时间、总住院时间及28天病死率与对照组比较,差异均无统计学意义（P>0.05）。结论 较单次缓慢静脉滴注氢化可的松,持续静脉微量泵入氢化可的松更能够稳定脓毒性休克患者血糖波动,有利于平衡机体代谢,而在稳定血流动力学、改善患者预后等方面并没有显著优势。     

HBXIP protein overexpression predicts the poor prognosis of pancreatic ductal adenocarcinomas

Background: Hepatitis B virus X-interacting protein (HBXIP) is associated with a variety of tumors. The purpose of this study was to investigate the clinicopathological significance of HBXIP expression in pancreatic ductal adenocarcinoma (PDAC) and to explore its potential as a biomarker for PDAC.Methods: Immunohistochemical (IHC) staining was performed on 126 PDAC tissues, 36 paraneoplastic tissues and 22 normal pancreatic tissues. The relationship between high levels of HBXIP expression and pathological features of PDAC patients was evaluated by chi-squared values.Results:The positive rate of HBXIP protein in PDAC tissues was 85.7% (108/126), which was significantly higher than that of adjacent pancreatic tissue (41.7%, 15/36) and normal pancreas (18.2%, 4/22). In addition, strong positive expression of HBXIP was associated with tumor size, positive lymph node metastasis, clinical stage and 80-month overall survival. Patient's age, gender, degree of differentiation, Ki-67 expression index, and calcification were, however, not associated with high levels of HBXIP expression.Conclusions:We present association between HBXIP expression and the pathological features of patients with PDAC.     

汶川地震后6个月安县受灾群众创伤后应激障碍(PTSD)的发生率及相关因素的分析

目的:汶川地震震后6个月安县受灾群众创伤后应激障碍(PTSD)发生率、症状分布及其相关因素。方法:应用PTSD调查表、症状自评量表(SCL-90)、焦虑自评量表、抑郁自评量表和标准化评定工具,对地震时在安县地区居住的群众,进行DSM-ⅣPTSD诊断检查及相关量表的测查。结果:完成调查的289人中,PTSD筛查阳性率为11.4%。抑郁症状阳性率为23.4%,8.7%报告有自杀观念。灾后6个月PTSD发生率为11.4%,PTSD组反复更现创伤性体验、持续性警觉性增高、持续的回避等症状较对照组明显。SCL-90总分和躯体化、强迫、人际关系、抑郁、焦虑、敌对、恐怖、偏执、精神及其他因子分、SDS总分和标准分、SAS总分和标准分均显著高于对照组。结论:地震后PTSD的发生率是11.4%(6个月)。PTSD患者无法摆脱精神创伤的痛苦记忆,PTSD严重影响了患者的心身健康。     

Risk of cardiovascular disease,death, and renal progression in diabetes according to albuminuria and estimated glomerular filtration rate

AimWe aimed to examine risks of major cardiovascular events (MACEs), renal outcomes, and all-cause mortality in type 2 diabetes mellitus (T2DM) patients with different diabetic kidney disease (DKD) subtypes.MethodsA total of 36,509 participants with T2DM recruited from 20 community sites across mainland China were followed up during 2011-2016. DKD subtypes were categorized based on albuminuria (urinary albumin-to-creatinine ratio, UACR ≥ 30 mg/g) and reduced estimated glomerular filtration rate (eGFR < 60 ml/min/1.73 m²) as Alb^?/eGFR^?, Alb⁺/eGFR^?, Alb^?/eGFR⁺, and Alb⁺/eGFR⁺. Cox proportional hazard models were used to calculate hazard ratios (HRs) and 95% confidence intervals (95% CIs) of developing clinical outcomes in DKD subtypes.ResultsMore than half (53.5%) of participants with diabetes and reduced eGFR had normal UACR levels (Alb^?/eGFR⁺), termed as non-albuminuria DKD. These patients had a modest increase in the risks of MACEs (hazard ratio, HR 1.42 [95% CI 1.08;1.88]) and mortality (HR 1.42 [1.04;1.92]) compared with patients without DKD, whereas CKD progression was not significantly increased (HR 0.97 [0.60;1.57]). Participants with albuminuria (Alb⁺/eGFR^? or Alb⁺/eGFR⁺) had higher risks of clinical outcomes. Subgroup analysis revealed that the associations between non-albuminuria DKD and risks of MACEs and mortality were more evident in those aged <65 years.ConclusionNon-albuminuria DKD accounts for more than half of DKD cases with low eGFR in Chinese diabetes patients. Diabetes patients with albuminuria are at higher risks of developing clinical outcomes and warrant early intervention, as well as patients with non-albuminuria DKD with age < 65 years.     

Plasma creatine kinase B correlates with injury severity and symptoms in professional boxers

IntroductionEach year in the United States, approximately 1.7 million people sustain a traumatic brain injury (TBI). Of these TBI events, about 75 percent are characterized as being mild brain injuries. Immediately following TBI, a secondary brain damage persists for hours, days, and even months. Previously, detection of neuronal and glial biomarkers have proven to be useful to predict neurological outcomes. Here, we hypothesized that creatine kinase, brain (CKBB) is a sensitive biomarker for acute secondary brain injury in professional boxers.MethodsBlood (8 cc) was collected from the boxing athletes (n = 18) prior to and after competition (∼30 min). The plasma levels of CKBB were measured using the Meso Scale Diagnostic (MSD) electrochemiluminescence (ECL) array-based multiplex format. Additional data such as number of blows to the head and symptom score (Rivermead Post Concussion Symptoms Questionnaire) were collected.ResultsAt approximately 30 min after the competition, the plasma levels of CKBB were significantly elevated in concussed professional boxers and correlated with the number of blows to the head and symptom scores. Additionally, receiver operating curve (ROC) analysis yielded a 77.8% sensitivity and a specificity of 82.4% with an area under the curve (AUC) of 90% for CKBB as an identifier of secondary brain injury within this population.ConclusionThis study describes the detection of CKBB as a brain biomarker to detect secondary brain injury in professional athletes that have experienced multiple high impact blows to the head. This acute biomarker may prove useful in monitoring secondary brain injury after injury.